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Cellulase 생산균주의 Screening 및 Bacillus licheniformis CEL-2 유래 Cellulase 특성 규명
권은령 ( Eun Ryeong Kwon ),서정민 ( Jung Min Seo ),이옥진 ( Ok Jin Lee ),김준호 ( Jun Ho Kim ),정영호 ( Young Ho Jung ),김영수 ( Yeong Su Kim ),노홍균 ( Hong Kyoon No ),박창수 ( Chang Su Park ) 한국키틴키토산학회 2015 한국키틴키토산학회지 Vol.20 No.1
We carried out screening of microorganisms producing cellulase from Korean traditional fermented foods. As a result, we isolated 5 strains as cellulolytic microorganisms due to their ability of forming active zone on LB-Agar plate containing carboxymethylcellulose (CM-cellulose) as substrate with trypan blue. Among one of the strains, one strain was selected because its culture supernatant showed the highest cellulase activity for CM-cullulose. In the analysis of 16S rRNA gene sequence from the strain, the isolated strain showed 99% of identity with Bacillus licheniformis, therefore we named the strain Bacillus licheniformis CEL-II. The optimum pH and temperature of cellulase from B. licheniformis CEL-II were the highest activities at pH 5.0 and 60 o C, respectively. In the investigation of cellulase activity for several substrates such as CM-cellulose, alkali swollen cellulose, α-cellulose, Sigma Cellulose and Avicel, the cellulase from B. licheniformis CEL-II showed the highest activity for CM-cellulose. From the analysis of enzyme reaction products obtained from the hydrolysis of alkali swollen cellulose, the cellulase from B. licheniformis CEL-II was characterized to be an endo-type enzyme because the various size cellooligosaccharides were detected as main products on the analysis by thin-layer chromatography.
토양 유래 Bacillus subtilis 유래 Xylanase의 특성 규명
정영호 ( Young Ho Jung ),권은령 ( Eun Ryeong Kwon ),박창수 ( Chang Su Park ) 대구가톨릭대학교 자연과학연구소 2015 자연과학연구논문집 Vol.13 No.1
Bacillus sp. strain was isolated as a xylanase producing microorganism from environmental soil. The strain showed activity zone on the LB agar plate containing Beechwood xylan stained with trypan blue due to its xylanase activity. Based on the 16S rRNA gene sequence analysis, the strain was identified as a Bacillus subtilis. The xylanase from B. subtilis exhibited the highest activities at pH 5.0 and 50℃, and the Beechwood xylan was determined as a the most proper substrate of the enzyme.