http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
TPA로 분화된 U937 세포에서 사람 세포거대바이러스에 의한 c- jun Promoter 활성도의 변화
박정규(Jung Gyu Park),김대중(Dae Joong Kim),김진희(Jin Hee Kim),한태희(Tae Hee Han),황응수(Eung Soo Hwang),최명식(Myung Sik Choi),국윤호(Yoon Ho Gook),최성배(Sung Bae Choi),차창룡(Chang Ryong Cha) 대한바이러스학회 1999 Journal of Bacteriology and Virology Vol.29 No.2
Transient transfection assay has been done to evaluate whether the c-jun activation would be prerequisite to the induction of permissiveness against human cytomegalovirus using in vitro cell model in which U937 has been induced to express CD11b and CD14 to become potential monocyte/macrophage cells by TPA treatment. U937 cells were treated with 10 ㎛, 50 ㎛ or 100 ㎛ of TPA. The cell morphology change was observed and the expression of the CD11b and CD14 was confirmed by FACS. Differentiated cells were transfected with pJLuc reporter vector which contained the wild type murine c-jun promoter spanning the SP1, CTF, ATF/CREB and MEF-2 binding sites upstream of the firefly luciferase gene. After 48 hrs of transfection, the cells were infected with HCMV Towne strain and the luciferase activity was assessed at 1 h and 4 h pi. The transfection assay showed no activation of the c-jun promoter at 1 h pi, instead, it showed 2 times increase of the its activity at 4 h pi. There was no difference of the c-jun promoter activation between TPA treated and untreated U937 cells, implying that c-jun activation might not be prerequisite for allowing cells to be premissive to HCMV, although HCMV infection itself could activate c-jun promoter.