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      저자 : 구희주 ( Gu Hui Ju ) (검색결과 1 건)
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      • KCI등재SCOPUS

        한국의 조기난소부전 환자에서 Growth Differentiation factor-9 유전자의 변이 분석

        구희주 ( Gu Hui Ju ),김영문 ( Kim Yeong Mun ),장동욱 ( Jang Dong Ug ),이지영 ( Lee Ji Yeong ),손인숙 ( Son In Sug ),김수녕 ( Kim Su Nyeong ),박기현 ( Park Gi Hyeon ),( Andrew R. Zinn ) 대한산부인과학회 2003 Obstetrics & Gynecology Science Vol.46 No.5

        The clinical models for studying ovary-determining genes may be premature ovarian failure (POF). POF is a condition causing amenorrhea, hypoestrogenism, and elevated gonadotropins in women under 40 years old. FSH receptor, LH receptor, inhibin, GDF-9 (growth differentiation factor-9), BMP-15 (bone morphogenetic protein-15), DIAPH2 (diaphanous gene) and XPNPEP2 (X-prolyl aminopeptidase) genes were proposed as a possible candidate gene, but until recently, only mutations in FSH receptor, LH receptor and inhibin genes have been identified in POF patients. Therefore mutation screening of another POF gene necessary to reveal the principal causative genes of POF. Objective : The present study was performed to analyze the mutation of GDF-9 gene in Korean patient with POF and to investigate whether mutation of these gene is a likely main cause of POF. Methods : Eighty-six women with POF were studied and thirty-six normal women were enrolled as control. Mutation screening of these genes were performed by denaturing HPLC and were confirmed by automatic sequencing. Results : Three different mutations of GDF-9 gene were identified in Korean women with POF ; Arg3Cys mutation on the patient, Leu40Val mutation in one patient, Asp57Tyr mutation in 10 patients and 6 normal controls. Arg3Cys mutation and Leu40Val mutation were likely cause of disease. Frequencies of polymorphism in Korean women. All mutations was a novel mutation found in the present study. Conclusion : POF was resulted by mutations of GDF-9 gene, but mutations of GDF-9 gene are not likely main causes of POF because of low frequency of mutations.

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