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Murine Melanoma B16 세포주의 항암제감수성 시험에 관한 실험적 연구
구명삼,진우정 全北大學校 齒醫學硏究所 1992 전북치대논문집 Vol.10 No.1
Anticancer drug sensitivity assays were performed using cotorimetric[3-(4.5-dimethylthiazol-2-yl)-2, 5-diphenyl-tetrazolium bromide(MTT)] assay and dye exclusion assay <DEA> on cell line. Comparison of the MTT assay with DEA is essential prior to its use in clinical screening study. Murine melanoma B16 cell lines were exposed to bleomycin, CDDP, Vinblastine for one hour(2.0×10^4 viable cells/well) and then cultured for four days in MEM containing 10% bovine serum for dye exclusion assay. In [MTT] assay, cells to be tested were exposed for one hour and continuously(5.0×10^3 viable cells/well). Four Hour before the culture end, added the MTT on media measured the absorbance with an ELISA reader at a wavelength of 490㎚. The results were as follows ; 1. Cancer cells were sensitive to bleomycin, CDDP at all concentrations and bleomycin revealed relatively good cytotoxicity than that of CDDP in [DEA] assay(P<0.05). 2. In [MTT] assay, cancer cells were sensitive to high concentration of all drygs and medium concentration of vinblastine for 1 hour exposure(P<0.01). 3. In [MTT] assay, cancer cells were sensitive to medium and high concentration of all drugs and low concentration of VINB for vinblastine continuous exposure(P<0.01). 4. In the comparision of chemosensitivity test between [DEA] and [MTT] assay, the result was correlated(γ=0.99, P<0.05).
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백서 설 발생과정에 작용하는 Cyclins, Cyclin Dependent Kinases, CDK Inhibitors의 변화
구명삼(Myoung Sam Koo),진우정(Woo Jeong Jin) 대한구강악안면외과학회 1997 대한구강악안면외과학회지 Vol.23 No.4
The molecular mechanisms that regulate glossal muscle cell cycle and terminal differentiation remain largely unknown. To determine which cyclins, cyclin dependent kinases (CDKs), cyclin dependent kinase inhibitors (CKIs) are important for glossal cell proliferation, we have examined expression of cyclins CDKs, CKIs during normal glossal muscle development in the rat. All cyclins, CDKs, and KIP/CIP family of CKIs were highly expressed during fetal glossal muscle development, then they decreased at different rates after birth. While the mRNAs of cyclin D1, D3, E, A, and B decreased gradually m glossal muscle during all stages of development, the protein levels of these cyclins decreased differently in tongue during pre- and postnatal development. While the functionally active form of cyclin D1, cyclin D3 and E proteins were observed until 7 days after birth, cyclin A and B proteins were decreased more slowly. While the CDK4, CDK6, CDK2, cdc2, and proliferating cell nuclear antigen (PCNA) proteins were highly present during fetal glossal muscle development and gradually decreased during postnatal development. Particularly, cdc2 levels decreased markedly after birth. lmmunohistochcmical data for PCNA was consistent with Western blotting data for PCNA temporally and spatially. The mRNA and protein levels of p21, p27, and p57 were high, then their levels changed differently during glossal development. While the mRNA levels of p21 and p57 decreased gradually, the mRNA level of p27 did not change during glossal development. While the protein levels of p21 and p57 in tongue decreased markedly after birth, the protein levels of p27 increased slightly after birth, then decreased at adulthood. These findings suggest that the all cyclins and CDKs observed are involved in glossal muscle cell cycle, and reduction of cyclins and CDKs and induction of p21 are associated with the withdrawal of glossal muscle cell cycle after birth.
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