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Ye Seul Park(박예슬),Gun He Nam(남건희),Kyung Jo Jo(조경조),Hye Won Kawk(곽혜원),Je-Geun Yoo(유제근),Jin Dong Jang(장진동),Sang Moon Kang(강상문),Sang Yong Kim(김상용),Young Min Kim(김영민) 한국생물공학회 2019 KSBB Journal Vol.34 No.3
Collagen is decomposed by MMP-1, an enzyme induced by transcription factor activator protein 1 (AP-1) and matrix metalloproteinase-9 (MMP-9). Action of MMPs in inflammatory response promotes inflammatory cell movement and secretion, resulting in wrinkles on the skin. After using Bacillus sp. fermentation system and water, Artemisia vulgaris was fermented to prepare different solvent fractions using water, dichloromethane, hex ane, n-butanol, and ethyl acetate. These fractions were used to assess their effects on cell survival, wound healing, MMP-1/MMP-9 and procollagen type I C-peptide (PICP) expression, and skin turnover. MTT assay showed that cell viability of each treated group was 103% to 121%, indicating that A. vulgaris fractions were not toxic compared to control (cell viability: 100%). Wound healing assay revealed that wound healing ability in each treated group was 51% to 61%. This was lower than wound healing area in the control. Using RT-PCR, inhibition of MMP-1/MMP-9 gene expression was examined. As a result, each group treated with fraction showed reduced expression of both MMP-1 and MMP-9 compared to tumor necrosis factor-α (TNF-α) treatment group. Effects on collagen biosynthesis were analyzed using a PICP ELISA Kit. The group in which Artemisia vulgaris was treated increased collagen synthesis from 141 to 262ng/mL compared to the control group. Three-dimensional cell culture revealed that each fraction could increase skin wall formation. These results suggest that each fractions has anti-aging and anti-wrinkle effects on the skin, indicating its suitability as a functional material.
박예슬(Ye Seul Park),남건희(Gun He Nam),조경조(Kyung Jo Jo),곽혜원(Hye Won Kawk),김명진(Myeong Jin Kim),김종태(Jong Tae Kim),장승희(Seung Hee Jang),김민정(Min Jeong Kim),김영민(Young Min Kim) 한국생물공학회 2020 KSBB Journal Vol.35 No.1
Obesity is caused by a variety of reasons, such as diet, genetics, and environmental factors. Here the effect of Barley sprout extract on the differentiation of 3T3-L1 and the expression of adipogenesis was studied. MTT assay for cell toxicity was performed and found to be non-toxic at 3T3-L1, with a survival rate of 90% or higher in Barley sprout extract at all concentrations. The results of oil red O assay, after inducing differentiation by treating the fat formation with insulin, dexamethasone, and IBMX powder-inducing substances at 3T3-L1, showed that the Barley sprout extract inhibited fat formation. The results of a triglyceride assay for the determination of quantitative inhibition of fat formation showed 42.2% and 45.7% in Barley sprout extracts of 50% and 70%, respectively. Western blot was performed to identify the proteins involved in fat cell suppression, and it was found that there was a concentration-dependent decrease in C/EBPα, PPARγ, and p-ACC proteins. Thus, Barley sprout ethanol extract regulates the expression of protein involved in inhibiting fat cells, thereby reducing the differentiation of fat cells and triglyceride production and indicating that it is effective against obesity.