[나의 시 나의 시론] 난잎이 흔들리면 외 2편

고명호 고요아침 1996 시조시학 Vol.- No.-

부딪치다 외 1편

고명호 고요아침 2009 시조시학 Vol.- No.-

하늘연못 외 1편

고명호 고요아침 2006 시조시학 Vol.- No.-

물엿 첨가에 의한 저염 오징어 젓갈의 유통기간 연장에 관한 연구 : 1. 저염오징어 젓갈의 물엿첨가에 의한 수분활성 및 Brix의 변화 1. Water Activity and Brix by the Addition of Corn Syrup of Low Salted Squid-Jeotkal

고명호,장미순,신석우 여수대학교 1999 論文集 Vol.14 No.2

오징어 젓갈 3kg을 원통형 회전 교반기에 넣고 물엿을 각각 10.15.20.25 및 30% 첨가한 다음 10℃ 및 20℃에서 교반하면서 1시간 간격으로 AW와 Brix를 측정하여 물엿의 침투정도와 여기에 다른 제품의 관능검사를 조사한 결과는 다음과 같다. 1. 물엿 첨가 오징어어육의 Aw는 교반시간에 따라 서서히 감소하였고 액즙은 교반 1시간 이내에 급격히 증가하다가 시간의 경과에 따라 완만히 증가하여 평행상태에 도달하였고 brix는 오징어육에서 교반초기에 급격히 증가한후 교반 시간이 경과함에 따라 완만히 증가하였으나 액즙에서는 물엿첨가량이 증가할수록 서서히 감소하는 경향을 나타내었다. 2. 물엿 농도에 따른 오징어육과 액즙의 Aw 및 brix는 물엿 105, 155에서 6시간까지 평해상태에 도달하지 않았으나 205 첨가시 6시간, 25% 첨가시 5시간 30분, 30% 첨가시 5시간 만에 평행상태에 도달하였고 이때의 Aw 및 brix의 값은 각각 0.869, 0.859, 0.847과 41.2%, 41.6%, 42.4%로 물엿첨가량이 증가할수록 Aw는 감소하였고 brix는 증가하였다 3. 물엿첨가에 따른 저염오징어 젓갈의 관능검사결과 25% 첨가하였을 때 가장 좋은 결과를 얻었다. In experiment to prolong the period of storage on the low salted squid-jeotkal using corn syrup, water activity(Aw) and brix were measured while agitating by one hour interval at 10℃ and 20℃ after putting into cylindrical agitator 3kg of ripening squid-jeotkal and con syrup of 10, 15, 20, 25 and 30%. Aw of squid meat decreased slowly according to the time elapsed and juice increased rapidly within 1 hour. Brix of squid meat increased slowly according to the time elapsed after increasing rapidly in initial of agitation and juice showed slowly decreasing tendency according to addition quantity of corn syrup. Changes of Aw and brix depended on concentration of corn-syrup does not arrival at condition of equilibrium until 6 hours in 10 and 15%. But occasion added 20, 25 and 30 % arrived at condition of equilibrium within each 6 hours, 5 hour 30 minutes and 5 hours. Value of Aw and brix on each corn syrup concentration of this time were 0.869, 0.859, 0.847 and 41.2, 41.6, 42.4%, respectively. The result of sensory evaluation for low salted squid-jeotkal using corn syrup was best in the addition of 25% corn syrup.

피부에서 자가포식 기전의 역할과 이를 기반으로 한 새로운 생리활성 소재의 개발

윤석정,고명호,정세규 한국피부장벽학회 2022 한국피부장벽학회지 Vol.24 No.2

Autophagy is an evolutionarily conserved catabolic process and plays essential roles for maintaining tissue homeostasis, cellular energy level and removal of dysfunctional proteins or pathogens. A lot of experimental and clinical studies suggest that the functional impairment of autophagy, represented by reduced formation of autophagic vacuoles and improper fusion of these vacuoles with lysosomes, is known to be associated with aging process, including skin aging. Hence, modulation of autophagy signaling is currently suggested as a potential anti-aging regimen. In skin, in addition to the aging process, autophagy plays crucial (patho)physiologic roles in epidermal differentiation, immune functions, melanogenesis, UV protection, and hair cycle regulations. There have been many studies reporting identification of autophagy modulating compounds either from the small molecules or natural extracts, and assessing their potential benefits on skin health. Recently identified transcriptional and epigenetic regulation mechanism of autophagy signaling further expands the possible application of autophagy-based, personalized approaches for skin diseases. In this review, the pleiotropic roles of autophagy in diverse skin functions are briefly discussed. Possible cross-talk between epigenetic regulation and autophagy signaling, mainly through micro-RNAs, is also discussed.

피부에서 자가포식 기전의 역할과 이를 기반으로 한 새로운 생리활성 소재의 개발

윤석정,고명호,정세규 한국피부장벽학회 2022 한국피부장벽학회지 Vol.24 No.2

Autophagy is an evolutionarily conserved catabolic process and plays essential roles for maintaining tissue homeostasis, cellular energy level and removal of dysfunctional proteins or pathogens. A lot of experimental and clinical studies suggest that the functional impairment of autophagy, represented by reduced formation of autophagic vacuoles and improper fusion of these vacuoles with lysosomes, is known to be associated with aging process, including skin aging. Hence, modulation of autophagy signaling is currently suggested as a potential anti-aging regimen. In skin, in addition to the aging process, autophagy plays crucial (patho)physiologic roles in epidermal differentiation, immune functions, melanogenesis, UV protection, and hair cycle regulations. There have been many studies reporting identification of autophagy modulating compounds either from the small molecules or natural extracts, and assessing their potential benefits on skin health. Recently identified transcriptional and epigenetic regulation mechanism of autophagy signaling further expands the possible application of autophagy-based, personalized approaches for skin diseases. In this review, the pleiotropic roles of autophagy in diverse skin functions are briefly discussed. Possible cross-talk between epigenetic regulation and autophagy signaling, mainly through micro-RNAs, is also discussed.

窒素源을 달리한 肥料施用이 大豆의 窒素固定力과 收量에 미치는 影響

李相甲,朴愚喆,高明鎬,金進鎬 慶北大學校 農業科學技術硏究所 1988 慶北大農學誌 Vol.6 No.-

窒素源을 달리한 肥料施用이 大豆의 生育, 收量, 營養成分 및 蛋白質 消化率 等에 미치는 肥效는 다음과 같다. 大豆의 生育狀況은 施肥條件間에 대체로 類似하였으나 硫安區와 無窒素區에 있어서 節數가 적었다. 根瘤數와 무게는 硫安區와 無窒素區가 낮았고 그 밖의 處理區에 있어서는 차이가 보이지 않았다. 根瘤數 當 窒素固定力은 生育初期와 開花期에는 거의 비슷하였으나 pod 伸長期에서는 開發複肥區가 他處理區보다 비교적 높았다. 大豆의 100粒重 및 10a 當 收量은 開發複肥區가 他處理區보다 높았고 無窒素區는 窒素源 肥料의 施用區보다 대체로 낮았다. 또한 大豆의 粗蛋白質 함량은 窒素源 肥料의 施用區가 無窒素區에 비해 높았고 粗脂肪 함량은 오히려 낮았다. 粗灰分 함량은 施用區間에 대체로 비슷하였으나 粗纖維 함량은 旣存複肥區가 他處理區에 비해 높았다. Ca, P, Na, K, Mg, Zn, Mn 및 Cu 함량은 施肥條件間에 대체로 비슷하였으나 Fe 함량은 無窒素區가 窒素源 肥料의 施用區에 比해 월등히 낮았으며, 蛋白質 消化率은 無窒素區에서 가장 높았다. Experiment was conducted to elucidate the effect of various nitrogen fertilizers on the growth and yield of soybean cv.Backwoon in field, and to examine soybeans produced with different nitrogen fertilizers for their nutritional constituents and protein digestibility. Soybean plants were generally similar in growth among fertilizers applied, and plants with non-nitrogen and with ammonium sulfate showed tendency to have fewer internodes. Nodule number and nodule weight also were lower in plants with non-nitrogen and with ammonium sulfate. Nitrogen fixation ability per nodule number were similar at an early growth stage and at flowering stage treatments and were relatively higher with newly developed complex fertilizer at pod elongation stage. With newly developed complex fertilizer and with non-nitrogen, there were slight increase and in 100 grain weight and yield per 10a, respectively. Crude protein contents in soybeans with nitrogen fertilizers were higher than that with non-nitrogen, and the reverse were true for crude fat. Crude ash contents were relatively similar among the treatment. Crude fiber contents were higher with readily used complex fertilizer than with the others. The contents of inorganic constituents were of the same sort among the treatments, that of Fe with non-nitrogen being much lower than with the others. Protein digestibility was the highest in the non-nitrogen treated soybean.

물엿 첨가에 의한 젓갈의 유통기간 연장에 관한 연구 3. 물엿 첨가에 의한 저염오징어 젓갈 저장 중의 성분변화

장미순(Mi sun JANG),고명호(Byeong Ho KOH),신석우(Suk U SHIN) 전남대학교 수산과학연구소 1999 수산과학연구소논문집 Vol.8 No.-

For the shelf life extension according to the storage periods of 5, 15, and 25℃ in low salted squid-jeotkal using corn syrup 25%, this study carried out. In order to judge the value as a trademark in the course of the storing periods at 5℃, 15℃ and 25℃, volatile basic nitrogen (VBN), pH, NH₂-N, NH₃-N, water activity(AW), brix and general components were investigated. The results were as follow. The concentration of moisture, protein, Ash, Aw, VBN and salinity were little above in the control than in 25% corn syrup and seasoning but brix lowed in the control. the initial VBN of the control and additive at 5℃ was each 18.65 and 17.80㎎/100g but 78.95 and 42.21㎎/100g in the 50 days of storage. VBN of control at 15℃ was 100㎎/100g in the 25 days and additive 92.48㎎/100g in 45 days. These of control and additive at 25℃ were each 100㎎/100g in the 12 days and in the 18 days. pH of control at 15℃ and 25℃ lowed less than pH 5 in the 30 and 12 days, respectively. And it of additive at 5℃, 15℃, and 25℃ was pH 5.82 in the 50 days, 5.41 in the 45 days and less than pH 5 in the 18 days. NH₂-N and NH₃-N increased slowly at 5℃ and 15℃ but these of control and corn syrup increased as rapidly as each 400~650㎎ and 60~280㎎/100g in the storing periods of 30 days at 25℃.

Aquatide Activation of SIRT1 Reduces Cellular Senescence through a SIRT1-FOXO1-Autophagy Axis

임채진,이용문,강승구,Hyung W. Lim,신경오,정세규,허양훈,최수인,고명호,서호성,박병덕,박기돈,안정근,Yoshikazu Uchida,박경호 한국응용약물학회 2017 Biomolecules & Therapeutics(구 응용약물학회지) Vol.25 No.5

Ultraviolet (UV) irradiation is a relevant environment factor to induce cellular senescence and photoaging. Both autophagy- and silent information regulator T1 (SIRT1)-dependent pathways are critical cellular processes of not only maintaining normal cellular functions, but also protecting cellular senescence in skin exposed to UV irradiation. In the present studies, we investigated whether modulation of autophagy induction using a novel synthetic SIRT1 activator, heptasodium hexacarboxymethyl dipeptide-12 (named as Aquatide), suppresses the UVB irradiation-induced skin aging. Treatment with Aquatide directly activates SIRT1 and stimulates autophagy induction in cultured human dermal fibroblasts. Next, we found that Aquatide-mediated activation of SIRT1 increases autophagy induction via deacetylation of forkhead box class O (FOXO) 1. Finally, UVB irradiation-induced cellular senescence measured by SA-β-gal staining was significantly decreased in cells treated with Aquatide in parallel to occurring SIRT1 activation-dependent autophagy. Together, Aquatide modulates autophagy through SIRT1 activation, contributing to suppression of skin aging caused by UV irradiation.

물엿 첨가에 의한 저염 오징어 젓갈의 유통기간 연장에 관한 연구 2. 물엿첨가 저염오징어 젓갈 저장 중의 미생물상의 변화

신석우(Suk U SHIN),장미순(Mi sun JANG),고명호(Byeong Ho KOH) 전남대학교 수산과학연구소 1999 수산과학연구소논문집 Vol.8 No.-

In order to prolong the period of storage, corn syrup 25% was added in low salt fermented squid-jeotkal. During the storing periods of this squid-jeotkal at 5℃, 15℃ and 25℃, total viable bacteria, proteolytic bacteria and this genus according to the period of storage were investigated. The results were as follows. Viable bacteria in the adding section and control increased in number of 4.1×10⁴ and 8.1×10⁶ CFU/g in the period of 50 days of storage at 5℃, respectively. The adding section of 15℃ was 4.7×10⁶ CFU/g during storage of 55 days and control 1.2×10⁸ CFU/g in the course of 40 days. Viable bacteria at 25℃ was more increase than at 5℃ an 15℃. So viable bacteria of the adding section and control were each 3.6×10⁷ and 4.3×10⁸ CFU/g. Proteolytic bacteria increase slowly up to 50 days of storage at 5℃ and so viable bacteria was 3.6×10³ CFU/g in the adding section and 4.8×10⁴ CFU/g in the control. These were each 100 CFU/g in 40 days at 15℃ of control and in 30 day at 25℃ of the adding section. The isolated bacteria was only staphylococcus sp. in the adding section but bacillus sp. and pseudomonas sp. besides staphylococcus sp. appeared in control.