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        열풍형 PCR 열싸이클러 개발을 위한 열유동해석에 관한 수치적 연구

        강정석(Jungseok Kang),양시영(Siyoung Yang),조원창(Wonchang Cho),김형민(Hyung Min Kim) 한국전산유체공학회 2020 한국전산유체공학회지 Vol.25 No.4

        PCR (Polymerase chain reaction) is a method that amplifies a small sample of specific DNA to a large enough amount for examination. The thermal cycler creates an accurate temperature environment required for amplifing DNA in the PCR process. In this study, a quantitative heat flow analysis was conducted to create an accurate temperature environment and less temperature difference in the 96-well plate by generating uniform heat flow profile in the expansion channel shape of the thermal cycler. The shape of the thermal cycler expansion channel was modeled using the 5th order polynomial equation, commonly used to design the contraction section of a low-speed wind tunnel. Additionally, turbulent heat flow analysis in the 96-well plate thermal cycler was conducted to investigate the temperature difference on the 96-well plate affected by the outer wall shape of the thermal cycler. It was confirmed that the maximum temperature difference of the 96-well plate in the thermal cycler with the suggested expansion wall shape is less than 1K. Based on this study, It is also effective to further reduce the temperature difference by deflecting the air flow by installing a baffle within a range that does not affect the velocity uniformity around the outlet of the thermal cycler.

      • 열차운용원의 직무유형 및 직무부하

        한규민(Han Kyumin),고종현(Ko Jonghyun),정원대(Jung Wondea),강정석(Kang Jungseok) 한국철도학회 2007 한국철도학회 학술발표대회논문집 Vol.- No.-

        In order to prevent railway accidents due to human errors which have been recognized to be the most important cause in the railway accidents, human errors should have been controlled based on systematical analysis of the human errors, and countermeasures should be derived to reduce human error probability. Among several factors inducing human errors, task load (or task complexity) is representative. In order to reduce the human error, a systematic analysis should be undertaken to evaluate task load. In this study, task load according to task types of railway worker who are a safety critical staff have been quantitatively analyzed based on NASA-TLX(Task Load Index).

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