http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Donghwan Kim(김동환),Byeongmin Kang(강병민),Jinah Jang(장진아) 대한기계학회 2021 대한기계학회 춘추학술대회 Vol.2021 No.4
Ischemic heart diseases are the worldwide major reason of morbidity and mortality. 3D bioprinted heart patch is a promising therapeutic method for treatment of the heart diseases. Many researchers have recently developed the engineered heart patches via extrusion-based 3D bioprinting technique, and they could reduce scarring, improve cardiac function to repair damaged heart and show high stability after implantation. However, they have limitations in using high viscous bioink which can cell functionality due to high mechanical properties compared with low viscous bioink. To solve these limitations, in this study, we present microfluidic 3D bioprinting technique to easily fabricate heart patch using low viscous bioink which can improve cell functionality and high-resolution printability through in situ crosslinking of alginate shell. We fabricated the heart patch composed of human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) with low viscous heart tissue-derived decellularized extracellular matrix (hdECM) at the core and alginate were located at the shell. and it shows high cell functionality compared with alginate-based heart patch. Furthermore, the suggested approach can facilitate the fabrication of multi-cellular heart patches composed of hiPSC-CMs core and endothelial cells shell that can improve the integration of heart patch with host’s vasculature.