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趙南奭,閔斗植 충북대학교 농업과학기술연구소 1991 農業科學硏究 Vol.9 No.1
Cellulosic biomass is potentially a major source of raw material for chemicals, food, and fuel in the future. There is a variety of waste cellulosic materials to be expected for many useful products. In Korea, approximately 4.03 million tons of paper and paper products are produced annually, and about 39% of those are finally discarded and incinerated. The aim of this study is to investigate the susceptibility of various waste paper resources to enzymatic saccharification with cellulase. Various grades papers, such as writing papers, paper boards and tissues, are tested. Their hydrolysis rates are ranged from 13.6% to 77.9%. Papers essentially consisting of high grade chemical pulps show the excellant hydrolysis rates due to their lower lignin contents. Newsprint shows very low saccharification rate because of its high groundwood including much lignin. Also activity of cellulase enzyme greatly affected to the saccharification. Concerning recycling and reutilization of cellulase enzyme, an improved almost quantitative recycle process of cellulase was discussed.
趙南奭 충북대학교 농업과학기술연구소 1990 農業科學硏究 Vol.8 No.2
Lignocellulosic materials which are the most abundant resources of organic carbon on the earth have the potential of providing for many useful products. As polysaccharides in lignocellulosic materials are encrusted with aromatic lignin molecules and have high crystallinity, these require pretreatment to improve their digestability by cellulolytic enzymes. Though a number of pretreatment methods have been proposed, the autohydrolysis process or steam explosion process is evaluated as a promising method. The steam explosion process breaks down the structure of wood and other lignocellulosics by hydrolyzing the hemicellulose component, cleaving lignin ether linkages and depolymerizing the lignin component, and enhances enzymatic hydrolysis. This study was performed to investigate the effect of various factors, such as treating conditions, moisture contents, particle size, lignin content and crystallinity of steam-exploded substrates, on the enzymatic hydrolysis. Wood chips were steam-exploded at high steaming pressure of 25-40kg/㎢ for 3-9min. This treatment greatly affected the succeptability of substrates on enzymatic hydrolysis. With the increase in steaming pressure and steaming time, the extent of enzymatic hydrolysis decreased. Particle sizes and moisture contents of substrates also were effective to the hydrolysis rate. The extents of hydrolysis were most effective at the conditions of 30kg/㎢, 3 min. treatment, at wet or air-dried state, and 80-100 meshes particle sizes. There was no direct relationship among lignin content, crystallinity of substrates and hydrolysis rate. Keywords : lignocellulosic material, steam-explosion, enzymatic hydrolysis, crystallinity