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HPLC에 의한 암환자의 요 중 아미노산 정량에 관한 연구
박택규,박수경,박정오 대한임상화학검사학회 1995 대한임상화학검사학회지 Vol.6 No.1
The concentration of free amino acids in urine of control groups and cancer patients were determined by reversed phase high performance liquid chromatography using pre-column derivatization with dansyl chloride and UV detection at 254 nm. Urine samples from the cancer patient of stomach. liver, gall bladder and pancreatic cancer showed a decrease of concentration in all amino acids except for methionine, isoleucine, glutamic acid and alanine as compared with the control groups. The concentration of alanine was most increased of all amino acids, whereas. that of lysine was most decreased. The decrease rate of essential amino acids was larger than that of nonessential amino acids. The concentration of essential amino acids compared with control groups in cancer patients decreased by 20-50%, whereas that of nonessential amino acids decreased by 15∼25%. The ratio of essential to nonessential amino acids (E/NE ratio) in control groups and cancer patients was 0.669 and 0.435, respectively. The ratio in cancer patients compared with control groups was decreased by 34.98%.