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Genetic Diversity of Orobanche cumana Populations in Serbia
Ž,arko Ivanović,Dragana Marisavljević,Radovan Marinković,Petar Mitrović,Jovana Blagojević,Ivan Nikolić,Danijela Pavlović 한국식물병리학회 2021 Plant Pathology Journal Vol.37 No.6
In this study, we report genetic characterization of Oro- banche cumana, the causal agent of sunflower wilting in Serbia. The genetic diversity of this parasitic plant in Serbia was not studied before. Random amplified polymorphic DNA (RAPD) markers and partial rbcL gene sequences analysis were used to characterize the O. cumana populations at the molecular level. While phylogenetic analyses of RAPD-PCR amplicons were performed using unweighted pair-group Method analy- ses, rbcL gene sequences were analyzed using neigbor joining method and minimum spanning tree. Molecular analyses of RAPD-PCR analysis revealed high genetic diversity of O. cumana populations which indicated high adaptive potential of this parasitic weed in Serbia. Further analyses of rbcL gene using minimum span- ning tree revealed clear differences among diverse sections of Orobanche genus. Although this molecular marker lacked the resolution to display intrapopulation diversity it could be a useful tool for understanding the evolution of this parasitic plant. Our results suggested that O. cumana has great genetic potential which can lead to differentiation of more virulent races which is important for determining crop breeding strategies for their control.
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Numerical analysis on the behaviour of reinforced concrete frame structures in fire
Igor M. Džolev,Meri J. Cvetkovska,Đorđe Ž. Lađinović,Vlastimir S. Radonjanin 사단법인 한국계산역학회 2018 Computers and Concrete, An International Journal Vol.21 No.6
Numerical approach using finite element method has been used to evaluate the behaviour of reinforced concrete frame structure subjected to fire. The structure is previously designed in accordance with Eurocode standards for the design of structures for earthquake resistance, for the ductility class M. Thermal and structural response are obtained using a commercially available software ANSYS. Temperature-dependent nonlinear thermal and mechanical properties are adopted according to Eurocode standards, with the application of constitutive model for the triaxial behaviour of concrete with a smeared crack approach. Discrete modelling of concrete and reinforcement has enabled monitoring of the behaviour at a global, as well as at a local level, providing information on the level of damage occurring during fire. Critical regions in frame structures are identified and assessed, based on temperatures, displacements, variations of internal forces magnitudes and achieved plastic deformations of main reinforcement bars. Parametric analyses are conducted for different fire scenarios and different types of concrete aggregate to determine their effect on global deformations of frame structures. According to analyses results, the threedimensional finite element model can be used to evaluate the insulation and mechanical resistance criteria of reinforced concrete frame structures subjected to nominal fire curves.
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