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Lim, Chae-Oh,Lee, Soo-In,Lee, Sang-Hyeung,Kim, Jong-Chel,Choi, Young-Ju,Bahk, Jeong-Dong,Lee, Sang-Yeol,Hwang, In-Hwan,Hong, Jong-Chan,Cho, Moo-Je Plant Molecular Biology & Biotechnology Research C 1994 Plant molecular biology and biotechnology research Vol.1994 No.
A cDNA clone encoding a cysteine proteinase inhibitor was obtained from ESTs of Chinese cabbage flower buds. The cDNA clone spans 784 base pairs, encoded 199-amino acid residues. BCPI contains the conserved Q-V-V-A-R sequence that exists in most members of the cystatin superfamily. Northern blot analysis showed that the amount of mRNA for BCPI was transcribe into a single mRNA species of about 800 nucleotides. BCPI was highly expressed in flower bud. Genomic southern blot analysis showed that BCPI is encoded by a small multigene family consist of at least two members. The protein expressed on E. coli inhibited strongly enzymatic activity of papain, a cysteine proteinase.
Lee, Won-Young,Hong, Joon-Ki,Kim, Cha-Young,Chun, Hyun-Jin,Park, Hyeong-Cheol,Kim, Jong-Cheol,Yun, Dae-Jin,Chung, Woo-Sik,Lee, Sung-Ho,Lee, Sang-Yeol,Cho, Moo-Je,Lim, Chae-Oh Plant molecular biology and biotechnology research 2003 Plant molecular biology and biotechnology research Vol.2003 No.-
A cDNA encoding RARF1 (rice ADP-ribosylation factor 1) was isolated from fungal elicitor-treated rice suspension culture cells by mRNA differential display. RARF1 transcripts accumulated in response to hydrogen peroxide (H_(2)O_(2)) and salicylic acid (SA) and rapidly in cells inoculated with an avirulent pathogen. Constitutively over-expressed RARF1 under the control of the cauliflower mosaic virus 35S promoter (CaMV 35S) triggered spontaneous induction of lesion mimics, induced an array of pathogenosis-related (PR) genes, reduced susceptibility to a fungal pathogen, and caused accumulation of SA. From these data, we deduced that RARF1 might be a component of various plant defence signaling pathways involved in inducing the expression of a subset of PR genes.
A Routine System for Generation of Fertile Transgenic Rice Plants Using Biolistic Method
Lee, Soo-In,Shon, Young-Geol,Kim, Cha-Young,Lim, Chae-Oh,Choi, Young-Ju,Kim, Ho-ll,Lee, Sang-Yeol,Lee, Sung-Ho Plant molecular biology and biotechnology research 2003 Plant molecular biology and biotechnology research Vol.2003 No.-
A routine system based on particle bombardment of embryogenic callus for recovery of fertile transgenic rice (Oryza sativa L.) plants was developed. Embryogenic callus was established within 2-3 months from calli derived from mature seeds of Korean rice cultivar, Nagdongbyeo. The callus was bombarded with the plamid pRQ6 containing the β-glucuronidase gene (gusA) and hygromycin phosphotransferase gene (hph, conferring resistance to hygromycin B), both driven by CaMV 35S promoter. Placement of cells on an osmoticum-containing medium (0.2 M sorbitol and 0.2 M mannitol) 4 hrs prior to and 16 hrs after bombardment resulted in a statistically significant increase with 3.2-fold in transient expression frequency gusA. In five independent experiments, the average frequency of transformation showing GUS activities was 8.86%. A large number of morphologically normal, fertile transgenic rice plants were obtained. Integration of foreign gene into the genome of R_(0) transgenic plants was confirmed by Southern blot analysis. GUS and HPT were detected in R_(1) progeny and Mendelian segregation of these genes was observed in R_(1) progeny.
Lim, Seungho,Yoon, Hong Yeol,Jang, Hee Jeong,Song, Sukyung,Kim, Woojun,Park, Jooho,Lee, Kyung Eun,Jeon, Sangmin,Lee, Sangmin,Lim, Dong-Kwon,Kim, Byung-Soo,Kim, Dong-Eog,Kim, Kwangmeyung American Chemical Society 2019 ACS NANO Vol.13 No.10
<P>Noninvasive and precise stem cell tracking after transplantation in living subject is very important to monitor both stem cell destinations and their <I>in vivo</I> fate, which is closely related to their therapeutic efficacy. Herein, we developed bicyclo[6.1.0]nonyne (BCN)-conjugated glycol chitosan nanoparticles (BCN-NPs) as a delivery system of dual-modal stem cell imaging probes. Near-infrared fluorescent (NIRF) dye Cy5.5 was chemically conjugated to the BCN-NPs, and then oleic acid-coated superparamagnetic iron oxide nanoparticles (OA-Fe<SUB>3</SUB>O<SUB>4</SUB> NPs) were encapsulated into BCN-NPs, resulting in Cy5.5-labeled and OA-Fe<SUB>3</SUB>O<SUB>4</SUB> NP-encapsulated BCN-NPs (BCN-dual-NPs). For bioorthogonal labeling of human adipose-derived mesenchymal stem cells (hMSCs), first, hMSCs were treated with tetra-acetylated <I>N</I>-azidoacetyl-<SMALL>D</SMALL>-mannosamine (Ac<SUB>4</SUB>ManNAz) for generating azide (−N<SUB>3</SUB>) groups onto their surface <I>via</I> metabolic glycoengineering. Second, azide groups on the cell surface were successfully chemically labeled with BCN-dual-NPs <I>via</I> bioorthogonal click chemistry <I>in vitro</I>. This bioorthogonal labeling of hMSCs could greatly increase the cell labeling efficiency, safety, and imaging sensitivity, compared to only nanoparticle-derived labeling technology. The dual-modal imaging-guided precise tracking of bioorthogonally labeled hMSCs was tested in the photothrombotic stroke mouse model <I>via</I> intraparenchymal injection. Finally, BCN-dual-NPs-labeled hMSCs could be effectively tracked by their migration from the implanted site to the brain stroke lesion using NIRF/<I>T</I><SUB>2</SUB>-weighted magnetic resonance (MR) dual-modal imaging for 14 days. Our observation would provide a potential application of bioorthogonally labeled stem cell imaging in regenerative medicine by providing safety and high labeling efficiency <I>in vitro</I> and <I>in vivo</I>.</P> [FIG OMISSION]</BR>
Lee, Jang Yeol,Kim, Na Young,Shin, Dong Yun,Park, Hee-Young,Lee, Sang-Soo,Joon Kwon, S.,Lim, Dong-Hee,Bong, Ki Wan,Son, Jeong Gon,Kim, Jin Young Springer-Verlag 2017 JOURNAL OF NANOPARTICLE RESEARCH Vol.19 No.3
<P>Transition metals, such as iron (Fe)- or cobalt (Co)-based nanomaterials, are promising electrocatalysts for oxygen reduction reactions (ORR) in fuel cells due to their high theoretical activity and low cost. However, a major challenge to using these metals in place of precious metal catalysts for ORR is their low efficiency and poor stability, thus new concepts and strategies should be needed to address this issue. Here, we report a hybrid aciniform nanostructures of Fe nanofragments embedded in thin nitrogen (N)-doped graphene (Fe@N-G) layers via a heat treatment of graphene oxide-wrapped iron oxide (Fe2O3) microparticles with melamine. The heat treatment leads to transformation of Fe2O3 microparticles to nanosized zero-valent Fe fragments and formation of core-shell structures of Fe nanofragments and N-doped graphene layers. Thin N-doped graphene layers massively promote electron transfer from the encapsulated metals to the graphene surface, which efficiently optimizes the electronic structure of the graphene surface and thereby triggers ORR activity at the graphene surface. With the synergistic effect arising from the N-doped graphene and Fe nanoparticles with porous aciniform nanostructures, the Fe@N-G hybrid catalyst exhibits high catalytic activity, which was evidenced by high E-1/2 of 0.82 V, onset potential of 0.93 V, and limiting current density of 4.8 mA cm(-2) indicating 4-electron ORR, and even exceeds the catalytic stability of the commercial Pt catalyst.</P>
Yeol-Lim Lee,Dae-Woon Jeong,Hyun-Seog Roh,Jeong-Geol Na,Sang Sub Han,Chang Hyun Ko 한국폐기물자원순환학회 2013 한국폐기물자원순환학회 학술대회 Vol.2013 No.2
Ce(1-x)Zr(x)O2 catalysts were investigated for bio-diesel production from oleic acid using catalytic deoxygenation. In this study, deoxygenation reaction has been carried out at 300 oC under 1 bar of 20% H2/N2 pressure in batch mode. Ce(1-x)Zr(x)O2 catalysts were prepared by co-precipitation method. Ce0.6Zr0.4O2 catalyst showed the highest oleic acid conversion and C9~C17 selectivity. It has been found that the deoxygenation reaction depends strongly on the reduction property and depends partly on the crystallite size of Ce(1-x)Zr(x)O2. Thus, Ce0.6Zr0.4O2 can be selected as the most promising catalyst for deoxygenation reaction.
Lee, Ho-Sun,Yoo, Dong-Lim,Ryu, Seung-Yeol,Sung, Jeong-Suk,Baek, Hyung-Jin,Lee, Young-Yi,Lee, Sok-Young The Plant Resources Society of Korea 2011 한국자원식물학회지 Vol.24 No.3
This experiment was carried out to investigate the appropriate chilling requirements for breaking dormancy by treating the dormant plant of Hanabusaya asiatica with low temperature ($4^{\circ}C$) for different time periods. The rates of sprouting and flowering were higher with longer treatment periods at low temperature. In addition, the growth and flowering of the plant were better when it was potted after treatment at a low temperature for 90 days. The abscisic acid levels and polyphenoloxidase activity of the dormant plant increased during the low temperature treatment, reached a climax 90 days and decreased thereafter. The catalase activity was the lowest after the low temperature treatment for 90 days and increased subsequently. The peroxidase activity increased and showed a sharp rise after the low temperature treatment for more than 90 days. Considering the physiological activities of the enzymes, the changes in the abscisic acid levels, and the characteristics of growth and flowering after sprouting of the plant, the appropriate cold periods required for breaking dormancy could be 90 days.
Molecular and functional characterization of a PEX14 cDNA from rice
Lee, Jung-Ro,Lee, Kyun-Oh,Park, Jin-Ho,Yoo, Ji-Young,Kang, Jae-Sook,Jeon, Hye-Sook,Kim, Sun-Young,Lee, Young-Mi,Kim, Sun-Tae,Lim, Chae-Oh,Bahk, Jeong-Dong,Cho, Moo-Je,Lee, Sang-Yeol Plant molecular biology and biotechnology research 2003 Plant molecular biology and biotechnology research Vol.2003 No.-
In contrast to the translocation mechanisms determined in yeasts and mammalian cells, there is little information on the functions of plant peroxisomal proteins or their genomic structures. To understand the role that PEX14 plays in diverse plant peroxisomal functions and how peroxisomal translocation is mediated in plant cells, we cloned a 1827 bp cDNA encoding the peroxisomal membrane protein OsPex14p from a rice leaf cDNA library. The 54kDa OsPex14p, which has a theoretical pI value of 6.06, contains a highly conserved N-terminal domain and a short putative transmembrane domain. The OsPEX14 gene in the rice genome exists as a single-copy gene, consists of eleven exons interrupted by ten introns, and spans about 5kb of chromosome 5. The 5′-flanking region contains putative cis-acting light-responsive elements, and the transcription initiation site maps 114bp upstream of the translation start codon. OsPEX14 mRNA is highly expresssed in leaf tissues and is induced by exposure to several stresses. Heterologous expression of OsPex14p suppresses the defect in targeting of peroxisomal matrix proteins in a pex14 null mutant of Saccharomyces cerevisiae.