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Genome Constitution and Classification Using Retrotransposon-Based Markers in the Orphan Crop Banana
( Chee How Teo ),( Siang Hee Tan ),( Chai Ling Ho ),( Qamaruz Zaman Faridah ),( Yasmin Rofina Othman ),( John Seymour Heslop Harrison ),( Ruslan Kalendar ),( Alan Howard Schulman ) 한국식물학회 2005 Journal of Plant Biology Vol.48 No.1
We have exploited the repetitive and dispersed nature of many long terminal repeat (LTR)-retrotransposon families for characterizing genome constitutions and classifying cultivars of the genus Musa. Insertional polymorphisms of the elements were studied using seven published and two newly designed primers facing outwards from the LTRs and reverse transcriptase (RT) domain of the retrotransposon. The primers generated specific amplification patterns showing the universal applicability of this marker type. The Inter-Retrotransposon Amplified Polymorphism (IRAP) markers distinguished the A and B genomes of the banana species (Musa acuminata Colla and Musa balbisiana Colla) and between banana cultivars. The IRAP markers enabled phylogenetic analysis of 16 Malaysian banana cultivars and determination of the genome constitution of hybrid banana (AAB, ABB, AABB, and AAAB), and gave information about ancestral genotypes of the hybrids. In addition, the TRAP detected new retrotransposon insertions into the genome of tissue culture regenerants. This PCR-based IRAP assay is amenable to large-scale throughput demands in screening breeding populations and is applicable for any crop.
Isolation and Characterization of Myb-Related Genes from Oil Palm (Elaeis guineensis Jacq.)
Teoh, Wan-Chin,Cheah, Suan-Choo,Harikrishna, Kulaveerasingam,Tan, Siang-Hee 한국식물학회 2003 Journal of Plant Biology Vol.46 No.2
Myb is a sequence-specific DNA-binding protein that can activate or inactivate promoters containing its binding site. Two different Myb-like genes were isolated from oil palm (Elaeis guineensis). The deduced amino acid sequence of OpMyb24, which was obtained from a zygotic embryo cDNA library, showed extensive homology to the snapdragon proteins Myb308 and Myb330, and to maize protein Zm38. Furthermore, the OpMyb75 gene, from an oil palm suspension culture (OPSC) cDNA library, was very similar to MIXTA from Antirrhinurn rnajus. OpMyb15 was expressed in all tissues tested, while transcript of OpMyb24 was detected everywhere except in three-month-old and young leaves. Although both transcripts accumulated to high levels in response to treatment with gibberellic acid, levels of OpMyb24 decreased significantly after plants were either wounded or treated with heavy metals or UV.