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- 저자 : ( Rishi Mahajan ) (검색결과 1 건)
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( Sampan Attri ),( Rishi Mahajan ),( Gunjan Goel ) 대한장연구학회 2018 Intestinal Research Vol.16 No.4
Background/Aims: The initial microbial colonization is a crucial step for the healthy development of an infant. Previous studies from India reported the dominance of target microbial species among Indian infants without any analysis on the diver sity of target groups. This is the first study from India with an objective to investigate the establishment and diversity of lactic acid producing bacteria (LAB) and bifidobacteria in vaginally delivered, full term, breastfed infants for the first 4 months after birth. Methods: Present study used polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) based sequence analysis of LAB and bifidobacteria in healthy infants. The results were used to compare the development and early colonization by LAB and bifidobacteria using diversity indices during the initial months of development of gut microbiota in infants. Results: During the first 4 months, the Shannon diversity index (H) of LAB increased from 1.16 to 1.318 and for bifido bacteria the H increased from 0.975 to 1.293 (P<0.05). Higher Sorenson’s pair wise similarity coefficient was observed for LAB and bifidobacteria during 2nd and the 3rd month. The species of the genera Enterococcus, Streptococcus, and Lactobacillus were dominant among the LAB group whereas Bifidobacterium breve was dominant species among Bifidobacterium group. Conclusions: Our results indicate that in breast fed infants, the microbial diversity of LAB and bifidobacteria increased during the period of study. (Intest Res 2018;16:529-536)
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