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( Zhenyue Feng ),( Defu Liu ),( Ziwen Liu ),( Yimin Liang ),( Yanhong Wang ),( Qingpeng Liu ),( Zhenhua Liu ),( Zhongjing Zang ),( Yudong Cui ) 한국미생물 · 생명공학회 2020 Journal of microbiology and biotechnology Vol.30 No.7
A putative multidrug efflux gene, yddA, was cloned from the Escherichia coli K-12 strain. A drugsensitive strain of E. coli missing the main multidrug efflux pump AcrB was constructed as a host and the yddA gene was knocked out in wild-type (WT) and drug-sensitive E. coliΔacrB to study the yddA function. Sensitivity to different substrates of WT E.coli, E. coliΔyddA, E. coliΔacrB and E. coliΔacrBΔyddA strains was compared with minimal inhibitory concentration (MIC) assays and fluorescence tests. MIC assay and fluorescence test results showed that YddA protein was a multidrug efflux pump that exported multiple substrates. Three inhibitors, ortho-vanadate, carbonyl cyanide m-chlorophenylhydrazone (CCCP), and reserpine, were used in fluorescence tests. Ortho-vanadate and reserpine significantly inhibited the efflux and increased accumulation of ethidium bromide and norfloxacin, while CCCP had no significant effect on YddA-regulated efflux. The results indicated that YddA relies on energy released from ATP hydrolysis to transfer the substrates and YddA is an ABC-type multidrug exporter. Functional study of unknown ATP-binding cassette (ABC) superfamily transporters in the model organism E. coli is conducive to discovering new multidrug resistance-reversal targets and providing references for studying other ABC proteins of unknown function.
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An innovative liquid metal-based pressure sensor with its application in geotechnical engineering
Yundong Shou,Xiaoping Zhou,Qingpeng Chang,Chao Liu 국제구조공학회 2021 Smart Structures and Systems, An International Jou Vol.27 No.1
In this paper, a monitoring system containing a novel liquid metal-based pressure sensor and remote interactive monitoring devices are fabricated to monitor stress in geotechnical engineering. The pressure sensor with the dimension of 34 mm × 34 mm is designed and manufactured, which is mainly composed of 40CrMoV alloy steel shell, polydimethylsiloxane (PDMS) and liquid metal gallium indium alloy (EGaIn). It has the characteristics of high stability, can greatly improve the measurement range of the pressure sensor (0 to 20 MPa), and its resistance variation is from 0 mΩ to 800 mΩ. In addition, the linear regression analysis is carried out to verify the linear relationship between the resistance of sensor and the applied pressure. It is found that the performance of the pressure sensor is fine through experiments with three different loading rates and fatigue experiments. The remote interactive monitoring device can be suitable for the field monitoring environment, which is composed of monitoring stations (MS), remote communication base stations (RCBS) and indoor working platform. Finally, the monitoring system is successfully applied to the field measurements in geotechnical engineering, and the field monitoring data are in good agreement with the numerical results.
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