Mitochondrial DNA Diversity and Origin of Red Chittagong Cattle

Bhuiyan, M.S.A.,Bhuiyan, A.K.F.H.,Yoon, D.H.,Jeon, J.T.,Park, C.S.,Lee, J.H. 충남대학교 형질전환복제돼지연구센터 2007 논문집 Vol. No.10

To determine the origin and genetic diversity of Red Chittagong (RC) cattle in Bangladesh, we analyzed mitochondrial DNA displacement loop (D-loop) sequences of 48 samples along with 22 previously published sequences from Bos indicus and Bos taurus breeds. Twenty five haplotypes were identified in RC cattle that were defined by 44 polymorphic sites and nucleotide diversity was 0.0055±0.0026. The estimated sequence divergence times between RC and other zebu cattle breeds studied ranged between 22,700-26,900 years before present (YBP) which, it is suggested, predate domestication of RC cattle. Furthermore, it is assumed that introgressions have occurred in this breed mainly from Indian zebu breeds in the recent millennia. The phylogenetic studies showed RC cattle clustered with Bos indicus lineage with two distinct haplogroups representing high genetic variability of this breed. These findings can be used for designing proper breeding and conservation strategies for RC cattle in Bangladesh.

Mitochondrial DNA Diversity and Origin of Red Chittagong Cattle

Bhuiyan, M.S.A.,Bhuiyan, A.K.F.H.,Yoon, D.H.,Jeon, J.T.,Park, C.S.,Lee, J.H. Asian Australasian Association of Animal Productio 2007 Animal Bioscience Vol.20 No.10

To determine the origin and genetic diversity of Red Chittagong (RC) cattle in Bangladesh, we analyzed mitochondrial DNA displacement loop (D-loop) sequences of 48 samples along with 22 previously published sequences from Bos indicus and Bos taurus breeds. Twenty five haplotypes were identified in RC cattle that were defined by 44 polymorphic sites and nucleotide diversity was $0.0055{\pm}0.0026$. The estimated sequence divergence times between RC and other zebu cattle breeds studied ranged between 22,700-26,900 years before present (YBP) which, it is suggested, predate domestication of RC cattle. Furthermore, it is assumed that introgressions have occurred in this breed mainly from Indian zebu breeds in the recent millennia. The phylogenetic studies showed RC cattle clustered with Bos indicus lineage with two distinct haplogroups representing high genetic variability of this breed. These findings can be used for designing proper breeding and conservation strategies for RC cattle in Bangladesh.

SELECTION FOR PROLIFICACY IN ROMNEY SHEEP II. CORRELATED RESPONSES

Bhuiyan, A.K.F.H.,Curran, M.K. Asian Australasian Association of Animal Productio 1995 Animal Bioscience Vol.8 No.1

A selection experiment with Romney Marsh sheep was used to evaluate correlated responses to selection. The selected flock was formed in 1979 by the Romney Group Breeders where selection was for prolificacy, defined as the number of live lambs born per ewe joined per year and a randomly selected control flock was established in 1982. Selection for prolificacy resulted in (i) increased ewe fertility, (ii) increased ewe ovulation rate, (iii) increased ewe litter size, (iv) decreased ewe body weight, (v) decreased lamb birth weight and (vi) decreased lamb 8-week weight. The rates of correlated responses per year respectively for ewe fertility, ewe ovulation rate, ewe litter size, ewe body weight, lamb birth weight and lamb 8-week weight were 0.033(0.002), 0.043(0.016), 0.019(0.005), -0.017(0.066), -0.055(0.025) and -0.150(0.057).

A Study of the Flow Phenomenon of Water in a Channel with Flat Plate Obstruction Geometry at the Entry

M. A. Kabir,M. M. K. Khan,M. A. Bhuiyan 대한기계학회 2003 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.17 No.6

The flow in a parallel walled test channel. when obstructed with a geometry at the entrance. can be forward. reverse and stagnant depending on the position of the obstruction. This interesting flow phenomenon has potential benefit in the control of energy and various flows in the process industry. In this experiment. the flat plate obstruction geometry was used as an obstruction at the entry of the test channel. The parameters that innuence the now inside and around the test channel were the gap (g) between the test channel and thc obstruction geometry. the Icngth (L) of the test channet and the Reynolds number (Re). The elrect of the gap to channel width ratio (g/w) on the magnitude of the velocity ratio (V₁/V_0: velocity inside/ velocity outside the test channel) was investigated for a range of Reynolds numbers. The maximum reverse now observed was nearly 20% to 60% of the outside velocity for Reynolds number ranging from 1000 to 9000 at g/w ratio of 1.5. The maximum forward velocity inside the test channel was found 80% of the outside velocity at higher g/w ratio of 8. The effect of the test channel length on the velocity ratio was investigated for different g/w ratios and a fixed Reynolds number of 4000. The innucnce of the Reynolds number on the velocity ratio is also discussed and presented for different gap to width ratio (g/w). The now visualisation photo-graphs showing fluid motion inside and around the test channel are also presented and discussed.

Radix Puerariae modulates glutamatergic synaptic architecture and potentiates functional synaptic plasticity in primary hippocampal neurons

Bhuiyan, M.M.,Haque, Md.N.,Mohibbullah, Md.,Kim, Y.K.,Moon, I.S. Elsevier Ireland Ltd 2017 Journal of Ethnopharmacology Vol.209 No.-

Ethnopharmacological relevance: Neurologic disorders are frequently characterized by synaptic pathology, including abnormal density and morphology of dendritic spines, synapse loss, and aberrant synaptic signaling and plasticity. Therefore, to promote and/or protect synapses by the use of natural molecules capable of modulating neurodevelopmental events, such as, spinogenesis and synaptic plasticity, could offer a preventive and curative strategy for nervous disorders associated with synaptic pathology. Radix Puerariae, the root of Pueraria monatana var. lobata (Willd.) Sanjappa&Pradeep, is a Chinese ethnomedicine, traditionally used for the treatment of memory-related nervous disorders including Alzheimer's disease. In the previous study, we showed that the ethanolic extracts of Radix Puerariae (RPE) and its prime constituent, puerarin induced neuritogenesis and synapse formation in cultured hippocampal neurons, and thus could improve memory functions. Aims of the study: In the present study, we specifically investigated the abilities of RPE and puerarin to improve memory-related brain disorders through modulating synaptic maturation and functional potentiation. Materials and methods: Rat embryonic (E19) brain neurons were cultured in the absence or presence of RPE or puerarin. At predetermined times, cells were live-stained with DiO or fixed and immunostained to visualize neuronal morphologies, or lysed for protein harvesting. Morphometric analyses of dendritic spines and synaptogenesis were performed using Image J software. Functional pre- and postsynaptic plasticity was measured by FM1-43 staining and whole-cell patch clamping, respectively. RPE or puerarin-mediated changes in actin-related protein 2 were assessed by Western blotting. Neuronal survivals were measured using propidium iodide exclusion assay. Results: RPE and puerarin both: (1) promoted a significant increase in the numbers, and maturation, of dendritic spines; (2) modulated the formation of glutamatergic synapses; (3) potentiated synaptic transmission by increasing the sizes of reserve vesicle pools at presynaptic terminals; (4) enhanced NMDA receptor-mediated postsynaptic currents, and (5) increased cell viability against naturally occurring cell death. Moreover, upregulation of actin-related protein 2 (ARP2) in RPE and puerarin treated brain neurons suggest that RPE and puerarin induced synaptic plasticity might be associated, at least in part, with ARP2-mediated actin-dependent regulation of spinogenesis. Conclusions: Our findings indicate that RPE and puerarin might play a substantial role in the morphological and functional maturation of brain neurons and suggest that RPE and puerarin are potentially valuable preventative therapeutics for memory-related nervous disorders.

SELECTION FOR PROLIFICACY IN ROMNEY SHEEP I. DIRECT RESPONSE TO SELECTION

Bhuiyan, A.K.F.H.,Curran, M.K. Asian Australasian Association of Animal Productio 1995 Animal Bioscience Vol.8 No.1

A selection experiment with Romney Marsh sheep was used to evaluate direct responses to selection. Two flocks were maintained; a) the selection line formed in 1979 by the Romney Group Breeders to select for high prolificacy, defined as the number of live lambs born per ewe joined per year and b) a control line, established in 1982, where flock replacements were chosen at random. Predicted responses per year of birth female group and per year respectively were 0.033 and 0.027 live lambs. The rate of predicted response per year was within the theoretical expected range from 0.01 to 0.03 of the mean. The rates of realized response in prolificacy per year of birth female group and per year respectively were 0.026 and 0.021. These estimates of realized responses represented between 0.01 and 0.02 of the control line mean per year.

Effect of Defined KSOM Medium on the Development of 1-antitrypsin Transgenic Nuclear Transfer Bovine Embryos

M.M.U. Bhuiyan,J.K. Cho,G. Jang,Park, E.S.,S.K. Kang,Lee, B.C.,W.S. Hwang 한국수정란이식학회 2002 한국수정란이식학회 학술대회 Vol.2002 No.1

Production of u 1-antitrypsin (AT) in transgenic cows has a great value in the field of medicine. The present study was conducted to determine the effect of chemically defined KSOM media on in vitro development of bovine transgenic nuclear transfer (NT) embryos. An expression plasmid for human AT was constructed by inserting a bovine beta-casein promoter, a green fluorescent protein (GFP) marker gene, and a human AT target gene into a pcDNA3 plasmid. Cumulus cells as donor nuclei in NT were collected from a Holstein cow and transfected by lipid-mediated method using FuGene6 (Roche Molecular Biochemicals, USA) as reagent. GFP expressed cumulus cells were introduced into recipient oocytes under DIC microscopy equipped with FITC filter set. After electrical fusion and chemical activation, reconstructed embryos were cultured in 1) SOF + 0.8% BSA, 2) KSOM + 0.8% BSA, 3) KSOM + 10% FBS and 4) KSOM +0.01% PVA for 192 h at 39 with 5% , 5% and 90% in humidified condition. The development of the embryos was recorded and the GFP expression in blastocyst was determined under FITC filter. The average fusion rate was 73.8% (251/340; n=8). The development rates to 2-4 cells, morula, blastocysts and expression rates in blastocysts varied from 70.3 to 76.5%, 30.2 to 33.8%, 25.4 to 33.8% and 11.8 to 15.6%, respectively. The difference in development and expression rates of embryos among 4 culture groups was not significant (P>0.05). This study indicates that chemically defined KSOM medium is also able to support development of bovine transgenic NT embryos at similar rate of SOF or KSOM supplemented with BSA or serum.

DNA Polymorphisms in SREBF1 and FASN Genes Affect Fatty Acid Composition in Korean Cattle (Hanwoo)

Bhuiyan, M.S.A.,Yu, S.L.,Jeon, J.T.,Yoon, D.,Cho, Y.M.,Park, E.W.,Kim, N.K.,Kim, K.S.,Lee, J.H. Asian Australasian Association of Animal Productio 2009 Animal Bioscience Vol.22 No.6

Sterol regulatory element binding factor 1 (SREBF1) and fatty acid synthase (FASN) genes play an important role in the biosynthesis of fatty acids and cholesterol, and in lipid metabolism. This study used polymorphisms in the intron 5 of bovine SREBF1 and in the thioesterase (TE) domain of FASN genes to evaluate their associations with beef fatty acid composition. A previously identified 84-bp indel (L: insertion/long type and S: deletion/short type) of the SREBF1 gene in Korean cattle had significant associations with the concentration of stearic (C18:0), linoleic (C18:2) and polyunsaturated fatty acids (PUFA). The stearic acid concentration was 6.30% lower in the SS than the LL genotype (p<0.05), but the linoleic and PUFA contents were 11.06% and 12.20% higher in SS compared to LL (p<0.05). Based on the sequence analysis, five single nucleotide polymorphisms (SNPs) g.17924G>A, g.18043C>T, g.18440G>A, g.18529G>A and g.18663C>T in the TE domain of the FASN gene were identified among the different cattle breeds studied. Among these, only g.17924 G>A and g.18663C>T SNPs were segregating in the Hanwoo population. The g.17924G>A SNP is a non-synonymous mutation (thr2264ala) and was significantly associated with the contents of palmitic (C16:0) and oleic acid (C18:1). The oleic acid concentration was 3.18% and 2.79% higher in Hanwoo with the GG genotype than the AA and AG genotypes, respectively (p<0.05), whereas the GG genotype had 3.8% and 4.01% lower palmitic acid than in those cattle with genotype AA and AG, respectively (p<0.05). Tissue expression data showed that SREBFI and FASN genes were expressed in a variety of tissues though they were expressed preferentially in different muscle tissues. In conclusion, the 84-bp indel of SREBF1 and g.17924G>A SNP of the FASN gene can be used as DNA markers to select Hanwoo breeding stock for fatty acid composition.

Ovarian Response to Different Dose Levels of Follicle Stimulating Hormone (FSH) in Different Genotypes of Bangladeshi Cattle

Ali, M.S.,Khandoker, M.A.M.Y.,Afroz, M.A.,Bhuiyan, A.K.F.H. Asian Australasian Association of Animal Productio 2012 Animal Bioscience Vol.25 No.1

The experiment was conducted under the Department of Animal Breeding and Genetics, Bangladesh Agricultural University (BAU), Mymensingh from June, 2001 to December, 2005 in two different locations (Central Cattle Breeding and Dairy Farm and Bangladesh Livestock Research Institute in Savar, Dhaka) to observe ovarian response to different doses of FSH in three different genotypes of cattle- indigenous Local, Pabna cattle and Friesian${\times}$Local cross. Five different dose levels used were 200, 240, 280, 320 and 360 mg. Ovarian response as corpus luteum (CL), recovered embryo (RE) and of transferable embryos (TE) count in Local were significant for 320, 280 and 280 mg respectively. In Pabna cattle CL, RE and TE count were found significant for 360, 320 and 320 mg respectively. In Friesian${\times}$Local cross CL, RE and TE count were found significant for 360, 320 and 320 mg respectively. The excellent quality embryos showed significantly the highest yield ($1.80{\pm}0.20$) in the 240 and 280 mg FSH levels in Local genotype. In Pabna cattle, the highest yield ($2.00{\pm}0.32$) was found at FSH level 320 mg. In Friesian${\times}$Local, the highest yield ($2.20{\pm}0.20$) was found at FSH level 280 mg.

인간 Prourokinase가 도입된 체세포를 이용한 소 형질전환 복제란 생산: 표지유전자 발현정도에 따른 효과

J. K. Cho,M.M.U. Bhuiyan,G. Jang,G. Jang,Park, E. S.,S. K. Kang,Lee, B. C.,W. S. Hwang 韓國受精卵移植學會 2002 한국동물생명공학회지 Vol.17 No.2

Human Prourokinase (proUK) offers potential as a novel agent with improved fibrin specificity and, as such, may offer advantages as an attractive alternative to urokinase that is associated with clinical benefits in patients with acute peripheral arterial occlusion. For production of transgenic cow as human proUK bioreacotor, we conducted this study to establish efficient production system for bovine transgenic embryos by somatic cell nuclear transfer (NT) using human prourokinase gene transfected donor cell. An expression plasmid for human prourokinase was constructed by inserting a bovine beta-casein promoter, a green fluorescent protein (GFP) marker gene, and human prourokinase target gene into a pcDNA3 plasmid. Cumulus cells were used as donor cell and transfected with the expression plasmid using the Fugene 6 as a carrier. To increase the efficiency for the production of transgenic NT, development rates were compared between non-transfected and transfected cell in experiment 1, and in experiment 2, development rates were compared according to level of GFP expression in donor cells. In experiment 1, development rates of non-transgenic NT embryos were significantly higher than transgenic NT embryos (43.3 vs. 28.4%). In experiment 2, there were no significant differences in fusion rates (85.4 vs. 78.9%) and cleavage rates (78.7 vs. 84.4%) between low and high expressed cells. However, development rates to blastocyst were higher in low expressed cells (17.0 vs. 33.3%), and GFP expression rates in blastocyst were higher in high expressed cells (75.0 vs. 43.3%), significantly.