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RISS 인기검색어
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- 저자 : ( Jilian Wang ) (검색결과 3 건)
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Wang Leiyu,Dong Kun,He Shaoyu,Li Jilian,Jie Wu,Huang Jiaxing 한국응용곤충학회 2021 Journal of Asia-Pacific Entomology Vol.24 No.1
Among the antimicrobial peptides, abaecin is rich in proline content and plays a vital role in insect innate im mune defense. Here, the full-length gene of abaecin from the bumblebee Bombus lantschouensis was cloned, and its expression profiles for different tissues, developmental stages and reproductive statuses were analyzed by RTqPCR. Meanwhile, the responses of abaecin to a bacterium (Escherichia coli) and a fungus (Beauveria bassiana) were tested. The full length of abaecin cDNA was 470 bp, and the open reading frame (ORF) was 258 bp, encoding a polypeptide of 85 amino acids. The abaecin gene consists of three exons and two introns. Phylogenetic analysis showed that Bombus ignitus was the closest species to B. lantschouensis base on putative Abaecin protein sequence. Expression analysis showed that abaecin was expressed broadly in different tissues, with the highest expression in fat bodies and extremely low expression in antennae. Regarding developmental stage, low expression of abacein was detected in eggs and larvae, and high expression was detected in pupal stages. The highest expression was observed at the Pw pupal stage (pupae with an unpigmented body cuticle and white eyes), and the expression then decreased from the Pp (pupae with pink eyes) to the Pdd (dark-eye pupae with a dark-pigmented cuticle) stages. In addition, the expression of abaecin was higher in egg-laying than in non-egglaying female bumblebees. Both E. coli and B. bassiana infections induced the expression of abaecin. Our results indicated that the abaecin gene plays important roles in the development, reproduction and immune responses of bumblebees. During the artificial rearing of bumblebees, a good environment should be created to avoid infection with bacteria or fungi.
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A novel reliability evaluation method on censored data
Anwei Shen,Jilian Guo,Zhuojian Wang,Wentong Jia 대한기계학회 2017 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.31 No.3
The effect of censored data on the average life of products was analyzed, and the relationships between the censored data and the reliability evaluation results were also given. A series of regression analysis function relationship was built to simplify the method of reliability evaluation on censored data. At a first step, a numerical simulation method was designed to create the random right censored data. Then the Bayesian and Markov chain Monte Carlo (MCMC) method was adopted to evaluate the reliability. Then we got the calculation deviation of Bayesian method under different sample sizes, different censored rates and different means of censored data. The relationships between the censored rates, the means of censored distribution and the reliability evaluation result were analyzed. Finally, we proposed the complete novel method through regression analysis. The corroboration of these relationships and the application to real life data demonstrate the advantages of the proposed methods.
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Isolation and Characterization of Cold- Adapted PGPB and Their Effect on Plant Growth Promotion
( Mingyuan Li ),( Jilian Wang ),( Tuo Yao ),( Zhenlong Wang ),( Huirong Zhang ),( Changning Li ) 한국미생물 · 생명공학회 2021 Journal of microbiology and biotechnology Vol.31 No.9
Cold-adapted plant growth-promoting bacteria (PGPB) with multiple functions are an important resource for microbial fertilizers with low-temperature application. In this study, culturable coldadapted PGPB strains with nitrogen fixation and phosphorus solubilization abilities were isolated. They were screened from root and rhizosphere of four dominant grass species in nondegraded alpine grasslands of the Qilian Mountains, China. Their other growth-promoting characteristics, including secretion of indole-3-acetic acid (IAA), production of siderophores and ACC deaminase, and antifungal activity, were further studied by qualitative and quantitative methods. In addition, whether the PGPB strains could still exert plant growth-promoting activity at 4°C was verified. The results showed that 67 isolates could maintain one or more growth-promoting traits at 4°C, and these isolates were defined as cold-adapted PGPB. They were divided into 8 genera by 16S rRNA gene sequencing and phylogenetic analysis, of which Pseudomonas (64.2%) and Serratia (13.4%) were the common dominant genera, and a few specific genera varied among the plant species. A test-tube culture showed that inoculation of Elymus nutans seedlings with cold-adapted PGPB possessing different functional characteristics had a significant growth-promoting effect under controlled low-temperature conditions, including the development of the roots and aboveground parts. Pearson correlation analysis revealed that different growth-promoting characteristics made different contributions to the development of the roots and aboveground parts. These cold-adapted PGPB can be used as excellent strain resources suitable for the near-natural restoration of degraded alpine grasslands or agriculture stock production in cold areas.
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