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Se-Jong Oh,Jae-Young Song,Jeongran Lee,Gi-An Lee,Ho-Cheol Ko,T. Stoilova,L. Krasteva,Yeon-Gyu Kim,Ju-Hee Rhee,Jae-Gyun Gwag,Na-Young Ro,On-Sook Hur,Myung-Chul Lee 한국국제농업개발학회 2012 韓國國際農業開發學會誌 Vol.24 No.5
본 연구는 농업유전자원센터에 보존되어있는 불가리아 재래종 고추 유전자원 61 점을 대상으로 농업형질을 조사하고, 22개의 분자마커(SSR marker)를 이용하여 불가리아 재래종 고추 유전자원의 유전적 다양성 및 집단분석을 통하여, 자원보존 및 효율적인 작물 육종을 위한 기초정보를 제공하고자 본실험을 수행하였으며 결과는 다음과 같다. 1. 파종 후 발아까지 소요일수는 최소 11일에서 최대 26 일,평균 16.9 일이었고 개화 소요일수는 최소 48일, 최대 65일, 평균 56.9일이었으며 성숙까지의 소요일수는 최소 73일, 최대 98일, 평균 90.7일이었다. 2. 농업형질의 특성을 바탕으로 PCA 분석을 이용하여 불가리아 고추의 다양성을 분석한 결과, 파종 후 개화까지의 소요일수에 따라 조생종, 중생종, 만생종 3개의 그룹으로 나눌 수있었다. 3. 61점의 고추자원에 대하여 22개 SSR 마커에 의해 나타난 대립유전자 (allele)수는 총 82개였다. 마커당 평균 allele수는 3.7 개였고, allele 수의 범위는 2개에서 5개로 확인되었다. 유전적 다양성을 나타내는 PIC 값의 범위는 0.061-0.636이었으며 평균 PIC 값은 0.349로 확인되었다. 4. 분자마커(SSR)를 이용하여 UPGMA, PCoA, STUCTURE 분석을 통한 고추의 다양성 및 집단 구조를 분석한 결과, 3개의 그룹으로 나뉘어졌다. 결론적으로, 농업형질 특성을 바탕으로 한 불가리아 재래종고추의 다양성과 분자학적 특성을 이용한 다양성 결과와는 차이가 있었다. The amount of genetic variability of a species is essential for its survival and adaptation in different environments, and studies of genetic diversity using molecular markers are necessary to understand the genetic structure of a population and to orientate effective strategies of germplasm conservation. The aim of current study was to determine the SSR markers that can be used rapidly and reliably to evaluated the pepper of Bulgaria landraces, and applied the markers to assessment of introduce genetic diversity of the pepper germplasm. We used 22 polymorphic microsatellite markers to analysis of genetic diversity within 61 pepper collection of Bulgaria landraces germplasm, all SSR primers pairs produced 82 polymorphic and reproducible amplification fragments. Results showed a relatively low level of genetic diversity among pepper accessions from Bulgaria for both molecular and morphological markers. An average value of polymorphic information contents (PIC) were 0.334 with a range of 0.061 to 0.63. The mean values of observed (HO) and gene diversity (GD) were 0.383 and 0.154, respectively, indicating a considerable amount of polymorphism within this collection. A genetic distance-based phylogeny grouped into three distinct groups according to genetic difference among the accessions. An average day of flowering time was 56.9 days with a range of 48 to 65 days. The average of fruit length andwidth were 6.9cm with a range 2.6 to 13.9cm, and 1.9cm with a range 0.5 to 4.2cm, respectively.
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( Geonwoo Lee ),( Minhyuk Kwon ),( Seungwoo Shin ),( Juseong Lee ),( Yunseo Choi ),( Jeongran Ko ),( Jae Yong Jang ),( Junghoon Kim ) 대한운동사협회 2022 대한운동사협회 운동사대회자료집 Vol.2022 No.-
OBJECTIVES ‘Ocean healing’ refers to treatments using seawater, seaweed, mud, sand, and all substances coming from the sea used for curative and preventative purposes. Previous studies have been supported the use of seawater to therapy in improving physical and mental health. However, the clinical effects of ocean healing program using leisure-sports remain unknown. Therefore, we investigated the effect of the ocean healing program using a stand-up paddle board (SUP) on body composition and dynamic balance in middle-aged women. METHODS Thirty women aged ≥30 years participated for this study and were randomly classified into SUP group (N=15, mean age: 50.0±13.5 years) and control group (N=15, mean age: 52.0±12.6 years). Body weight, body mass index, %body fat, skeletal muscle mass, and skeletal muscle index were measured using InBody. We also conducted the Y-balance test to measure the dynamic balance ability. Participants in the intervention group performed the SUP program 3 times a week for 60 min, and the total intervention period was 10 weeks. RESULTS In the intervention group, 13 women completed the 10-week intervention. Body weight (P=0.004) and %body fat (P=0.002) was significantly decreased in the SUP group after intervention. Moreover, we found that skeletal muscle mass was increased in SUP group (P=0.029) but not in control group. Compared to the control group, the score of Y-balance test of the intervention group were significantly improved (baseline: 85.6±7.6 vs post: 96.2±5.4, P<0.001). CONCLUSIONS On trial completion the primary outcome (body weight, %body fat, and skeletal muscle mass) was significantly improved compared with control group. We also found improved dynamic balance in the SUP group after 10 weeks intervention. Our findings suggest that implementation of an ocean healing program is an effective intervention to enhance body composition and dynamic balance in middle-aged women.
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