http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
A Review of Microbial Biofilms of Produce: Future Challenge to Food Safety
Iqbal Kabir Jahid,하상도 한국식품과학회 2012 Food Science and Biotechnology Vol.21 No.2
Outbreaks of produce-related food-borne pathogens have undergone a sharp increase in last three decades because of high produce consumption. A paradigm of food safety for produce is important due to its susceptibility to microbial attack and biofilms formation. Greater attention should be paid to decontaminating the pathogens in biofilms as they pose a risk to public health. This review will focus on produce-related outbreaks,attachments, quorum sensing, biofilms formation, resistance to sanitizers and disinfectants, and current and emerging control strategies for fresh and minimally processed produce, providing new insight into food safety. The consequences of biofilms formation on produce include the formation of a protective environment that is resistant to cleaning and disinfection. Alternative means of controlling or inhibiting biofilms formation on produce will be explained briefly and we will identify where additional research is needed.
Jahid, Iqbal Kabir,Mizan, Md. Furkanur Rahaman,Ha, Angela J.,Ha, Sang-Do Elsevier 2015 FOOD MICROBIOLOGY Vol.49 No.-
<P><B>Abstract</B></P> <P>The aim of this study was to determine the effect of salinity and age of cultures on quorum sensing, exoprotease production, and biofilm formation by <I>Aeromonas hydrophila</I> on stainless steel (SS) and crab shell as substrates. Biofilm formation was assessed at various salinities, from fresh (0%) to saline water (3.0%). For young and old cultures, planktonic cells were grown at 30?°C for 24?h and 96?h, respectively. Biofilm formation was assessed on SS, glass, and crab shell; viable counts were determined in R2A agar for SS and glass, but <I>Aeromonas-</I>selective media was used for crab shell samples to eliminate bacterial contamination. Exoprotease activity was assessed using a Fluoro™ protease assay kit. Quantification of acyl-homoserine lactone (AHL) was performed using the bioreporter strain <I>Chromobacterium violaceum</I> CV026 and the concentration was confirmed using high-performance liquid chromatography (HPLC). The concentration of autoinducer-2 (AI-2) was determined with <I>Vibrio harveyi</I> BB170. The biofilm structure at various salinities (0–3 %) was assessed using field emission electron microscopy (FESEM). Young cultures of <I>A.?hydrophila</I> grown at 0–0.25% salinity showed gradual increasing of biofilm formation on SS, glass and crab shell; swarming and swimming motility; exoproteases production, AHL and AI-2 quorum sensing; while all these phenotypic characters reduced from 0.5 to 3.0% salinity. The FESEM images also showed that from 0 to 0.25% salinity stimulated formation of three-dimensional biofilm structures that also broke through the surface by utilizing the chitin surfaces of crab, while 3% salinity stimulated attachment only for young cultures. However, in marked contrast, salinity (0.1–3%) had no effect on the stimulation of biofilm formation or on phenotypic characters for old cultures. However, all concentrations reduced biofilm formation, motility, protease production and quorum sensing for old culture. Overall, 0–0.25% salinity enhanced biofilm formation and expression of quorum sensing regulatory genes in young cultures, whereas these responses were reduced when salinity was >0.25%. In old cultures, salinity at any concentrations (0.1–3%) induced stress in <I>A.?hydrophila</I>. The present study provides insight into the ecology of <I>A.?hydrophila</I> growing on fish and crustaceans such as shrimp and crabs in estuarine and seawater.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Salinity controls biofilms formation by <I>Aeromonas hydrophila</I>. </LI> <LI> Planktonic age is important for controlling biofilms and quorum sensing. </LI> <LI> Salinity modulates quorum sensing, motility and exoprotease activity. </LI> <LI> Water salinity contributes to biofilms formation on crab shell by <I>A.?hydrophila</I>. </LI> </UL> </P>
Mizan, Md. Furkanur Rahaman,Jahid, Iqbal Kabir,Kim, Minhui,Lee, Ki-Hoon,Kim, Tae Jo,Ha, Sang-Do Taylor Francis 2016 BIOFOULING -CHUR- Vol.32 No.4
<P>Vibrio parahaemolyticus is one of the leading foodborne pathogens causing seafood contamination. Here, 22 V. parahaemolyticus strains were analyzed for biofilm formation to determine whether there is a correlation between biofilm formation and quorum sensing (QS), swimming motility, or hydrophobicity. The results indicate that the biofilm formation ability of V. parahaemolyticus is positively correlated with cell surface hydrophobicity, autoinducer (AI-2) production, and protease activity. Field emission scanning electron microscopy (FESEM) showed that strong-biofilm-forming strains established thick 3-D structures, whereas poor-biofilm-forming strains produced thin inconsistent biofilms. In addition, the distribution of the genes encoding pandemic clone factors, type VI secretion systems (T6SS), biofilm functions, and the type I pilus in the V. parahaemolyticus seafood isolates were examined. Biofilm-associated genes were present in almost all the strains, irrespective of other phenotypes. These results indicate that biofilm formation on/in seafood may constitute a major factor in the dissemination of V. parahaemolyticus and the ensuing diseases.</P>
Microbial biofilms in seafood: A food-hygiene challenge
Mizan, Md. Furkanur Rahaman,Jahid, Iqbal Kabir,Ha, Sang-Do Elsevier 2015 FOOD MICROBIOLOGY Vol.49 No.-
<P><B>Abstract</B></P> <P>Seafood forms a part of a healthy diet. However, seafood can be contaminated with foodborne pathogens, resulting in disease outbreaks. Because people consume large amounts of seafood, such disease outbreaks are increasing worldwide. Seafood contamination is largely due to the naturally occurring phenomenon of biofilm formation. The common seafood bacterial pathogens that form biofilms are Vibrio spp., Aeromonas hydrophila, Salmonella spp., and Listeria monocytogenes. As these organisms pose a global health threat, recent research has focused on elucidating methods to eliminate these biofilm-forming bacteria from seafood, thereby improving food hygiene. Therefore, we highlight recent advances in our understanding of the underlying molecular mechanisms of biofilm formation, the factors that regulate biofilm development and the role of quorum sensing and biofilm formation in the virulence of foodborne pathogens. Currently, several novel methods have been successfully developed for controlling biofilms present in seafood. In this review, we also discuss the epidemiology of seafood-related diseases and the novel methods that could be used for future control of biofilm formation in seafood.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Bacterial biofilms contaminate seafood. </LI> <LI> Most <I>Vibrio</I> spp. form biofilms on seafood. </LI> <LI> Numerous factors affect biofilm formation on seafood. </LI> <LI> Use of novel biofilm-reducing methods could minimize seafood-borne diseases. </LI> </UL> </P>
( Shovon Lal Sarkar ),( Md Iqbal Hossain ),( Sharmin Akter Monika ),( Santonu Kumar Sanyal ),( Pravas Chandra Roy ),( Md Anwar Hossain ),( Iqbal Kabir Jahid ) 한국미생물 · 생명공학회 2020 한국미생물·생명공학회지 Vol.48 No.3
Probiotics are live microorganisms that, when administered in adequate amounts, confer health benefits to the host. This study was conducted for the isolation of potential lactic acid bacteria (LAB) with probiotic properties from goat milk and yogurt. Several tests were conducted in vitro using the standard procedures for evaluating the inhibitory spectra of LAB against pathogenic bacteria; tolerance to NaCl, bile salt, and phenol; hemolytic, milk coagulation, and bile salt hydrolase activities; gastrointestinal transit tolerance; adhesion properties; and antibiotic susceptibility. Among 40 LAB strains screened according to culture characteristics, five isolates exhibited antagonistic properties. Three were identified as Pediococcus acidilactici, and two were identified as Enterococcus faecium, exploiting 16S rRNA gene sequencing. All the isolates succeeded in the gastrointestinal transit tolerance assay and successively colonized mucosal epithelial cells. Based on the results of these in vitro assays, both P. acidilactici and E. faecium can be considered as potential probiotic candidates.
Rine Christopher Reuben,Pravas Chandra Roy,Shovon Lal Sarkar,하상도,Iqbal Kabir Jahid 한국미생물·생명공학회 2019 한국미생물·생명공학회지 Vol.47 No.4
In the aquatic environment, microorganisms are predominantly organized as biofilms. Biofilms are formed by the aggregation of microbial cells and are surrounded by a matrix of extracellular polymeric substances (EPS) secreted by the microbial cells. Biofilms are attached to various surfaces, such as the living tissues, indwelling medical devices, and piping of the industrial potable water system. Biofilms formed from a single species has been extensively studied. However, there is an increased research focus on multispecies biofilms in recent years. It is important to assess the microbial mechanisms underlying the regulation of multispecies biofilm formation to determine the drinking water microbial composition. These mechanisms contribute to the predominance of the best-adapted species in an aquatic environment. This review focuses on the interactions in the multispecies biofilms, such as coaggregation, co-metabolism, cross-species protection, jamming of quorum sensing, lateral gene transfer, synergism, and antagonism. Further, this review explores the dynamics and the factors favoring biofilm formation and pathogen transmission within the drinking water distribution systems. The understanding of the physiology and biodiversity of microbial species in the biofilm may aid in the development of novel biofilm control and drinking water disinfection processes.