TSPAN12 Precedes Tumor Proliferation by Cell Cycle Control in Ovarian Cancer

Ji, Guohua,Liang, Hongbin,Wang, Falin,Wang, Nan,Fu, Songbin,Cui, Xiaobo Korean Society for Molecular and Cellular Biology 2019 Molecules and cells Vol.42 No.7

TSPAN12, a member of the tetraspanin family, has been highly connected with the pathogenesis of cancer. Its biological function, however, especially in ovarian cancer (OC), has not been well elucidated. In this study, The Cancer Genome Atlas (TCGA) dataset analysis revealed that upregulation of TSPAN12 gene expression was significantly correlated with patient survival, suggesting that TSPAN12 might be a potential prognostic marker for OC. Further exploration showed that TSPAN12 overexpression accelerated proliferation and colony formation of OVCAR3 and SKOV3 OC cells. Knockdown of TSPAN12 expression in A2780 and SKOV3 cells decreased both proliferation and colony formation. Western blot analysis showed that several cyclins and cyclin-dependent kinases (CDK) (e.g., Cyclin A2, Cyclin D1, Cyclin E2, CDK2, and CDK4) were significantly involved in the regulation of cell cycle downstream of TSPAN12. Moreover, TSPAN12 accelerated mitotic progression by controlling cell cycle. Thus, our data demonstrated that TSPAN12 could be a novel molecular target for the treatment of OC.

TSPAN12 Precedes Tumor Proliferation by Cell Cycle Control in Ovarian Cancer

Guohua Ji,Hongbin Liang,Falin Wang,Nan Wang,Songbin Fu,Xiaobo Cui 한국분자세포생물학회 2019 Molecules and cells Vol.42 No.7

TSPAN12, a member of the tetraspanin family, has been highly connected with the pathogenesis of cancer. Its biological function, however, especially in ovarian cancer (OC), has not been well elucidated. In this study, The Cancer Genome Atlas (TCGA) dataset analysis revealed that upregulation of TSPAN12 gene expression was significantly correlated with patient survival, suggesting that TSPAN12 might be a potential prognostic marker for OC. Further exploration showed that TSPAN12 overexpression accelerated proliferation and colony formation of OVCAR3 and SKOV3 OC cells. Knockdown of TSPAN12 expression in A2780 and SKOV3 cells decreased both proliferation and colony formation. Western blot analysis showed that several cyclins and cyclin-dependent kinases (CDK) (e.g., Cyclin A2, Cyclin D1, Cyclin E2, CDK2, and CDK4) were significantly involved in the regulation of cell cycle downstream of TSPAN12. Moreover, TSPAN12 accelerated mitotic progression by controlling cell cycle. Thus, our data demonstrated that TSPAN12 could be a novel molecular target for the treatment of OC.

Design and Synthesis of Acid-resistant Zeolite T/NaY Composite Membrane for Water/Ethanol Separation

Xiangbo Li,Zhihui Zhou,Liang Chen,Chunlong Kong,Hongbin Du 대한화학회 2019 Bulletin of the Korean Chemical Society Vol.40 No.6

The random distribution of zeolite T on the porous α-Al2O3 tube during growth greatly limits its application in the industry. In this study, the defective zeolite T layer is modified with zeolite NaY. A continuous and dense zeolite T/NaY composite membrane is obtained through characterization by scanning electron microscope and X-ray diffraction. The resulting composite membrane exhibits high selectivity and high permeation flux for 90?wt % ethanol/water solution at 75 °C. Water concentration in the permeation side of the as-synthesized zeolite T/NaY composite membrane is >99.9 wt %, whereas that of primary zeolite T layer is only 60.4 wt %. The water permeation flux of the composite membrane reaches 4.35?kg/m2/h1. The composite membrane displays good stability in acid solution when the pH of the solution is >5.

An Enhanced Privacy-Aware Authentication Scheme for Distributed Mobile Cloud Computing Services

( Ling Xiong ),( Daiyuan Peng ),( Tu Peng ),( Hongbin Liang ) 한국인터넷정보학회 2017 KSII Transactions on Internet and Information Syst Vol.11 No.12

With the fast growth of mobile services, Mobile Cloud Computing(MCC) has gained a great deal of attention from researchers in the academic and industrial field. User authentication and privacy are significant issues in MCC environment. Recently, Tsai and Lo proposed a privacy-aware authentication scheme for distributed MCC services, which claimed to support mutual authentication and user anonymity. However, Irshad et.al. pointed out this scheme cannot achieve desired security goals and improved it. Unfortunately, this paper shall show that security features of Irshad et.al.’s scheme are achieved at the price of multiple time-consuming operations, such as three bilinear pairing operations, one map-to-point hash function operation, etc. Besides, it still suffers from two minor design flaws, including incapability of achieving three-factor security and no user revocation and re-registration. To address these issues, an enhanced and provably secure authentication scheme for distributed MCC services will be designed in this work. The proposed scheme can meet all desirable security requirements and is able to resist against various kinds of attacks. Moreover, compared with previously proposed schemes, the proposed scheme provides more security features while achieving lower computation and communication costs.

Interconnected hollow carbon nanospheres for stable lithium metal anodes

Zheng, Guangyuan,Lee, Seok Woo,Liang, Zheng,Lee, Hyun-Wook,Yan, Kai,Yao, Hongbin,Wang, Haotian,Li, Weiyang,Chu, Steven,Cui, Yi Nature Publishing Group, a division of Macmillan P 2014 Nature nanotechnology Vol.9 No.8

For future applications in portable electronics, electric vehicles and grid storage, batteries with higher energy storage density than existing lithium ion batteries need to be developed. Recent efforts in this direction have focused on high-capacity electrode materials such as lithium metal, silicon and tin as anodes, and sulphur and oxygen as cathodes. Lithium metal would be the optimal choice as an anode material, because it has the highest specific capacity (3,860 mAh g<SUP>–1</SUP>) and the lowest anode potential of all. However, the lithium anode forms dendritic and mossy metal deposits, leading to serious safety concerns and low Coulombic efficiency during charge/discharge cycles. Although advanced characterization techniques have helped shed light on the lithium growth process, effective strategies to improve lithium metal anode cycling remain elusive. Here, we show that coating the lithium metal anode with a monolayer of interconnected amorphous hollow carbon nanospheres helps isolate the lithium metal depositions and facilitates the formation of a stable solid electrolyte interphase. We show that lithium dendrites do not form up to a practical current density of 1 mA cm<SUP>–2</SUP>. The Coulombic efficiency improves to ∼99% for more than 150 cycles. This is significantly better than the bare unmodified samples, which usually show rapid Coulombic efficiency decay in fewer than 100 cycles. Our results indicate that nanoscale interfacial engineering could be a promising strategy to tackle the intrinsic problems of lithium metal anodes.

Ultrathin Two-Dimensional Atomic Crystals as Stable Interfacial Layer for Improvement of Lithium Metal Anode

Yan, Kai,Lee, Hyun-Wook,Gao, Teng,Zheng, Guangyuan,Yao, Hongbin,Wang, Haotian,Lu, Zhenda,Zhou, Yu,Liang, Zheng,Liu, Zhongfan,Chu, Steven,Cui, Yi American Chemical Society 2014 NANO LETTERS Vol.14 No.10

<P>Stable cycling of lithium metal anode is challenging due to the dendritic lithium formation and high chemical reactivity of lithium with electrolyte and nearly all the materials. Here, we demonstrate a promising novel electrode design by growing two-dimensional (2D) atomic crystal layers including hexagonal boron nitride (h-BN) and graphene directly on Cu metal current collectors. Lithium ions were able to penetrate through the point and line defects of the 2D layers during the electrochemical deposition, leading to sandwiched lithium metal between ultrathin 2D layers and Cu. The 2D layers afford an excellent interfacial protection of Li metal due to their remarkable chemical stability as well as mechanical strength and flexibility, resulting from the strong intralayer bonds and ultrathin thickness. Smooth Li metal deposition without dendritic and mossy Li formation was realized. We showed stable cycling over 50 cycles with Coulombic efficiency ∼97% in organic carbonate electrolyte with current density and areal capacity up to the practical value of 2.0 mA/cm<SUP>2</SUP>and 5.0 mAh/cm<SUP>2</SUP>, respectively, which is a significant improvement over the unprotected electrodes in the same electrolyte.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/nalefd/2014/nalefd.2014.14.issue-10/nl503125u/production/images/medium/nl-2014-03125u_0005.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/nl503125u'>ACS Electronic Supporting Info</A></P>

Measurement of TOF of fast neutrons with ²³⁸U target

Li, Meng,Guan, Yuanfan,Lu, Chengui,Zhang, Junwei,Yuan, Xiaohua,Duan, Limin,Yang, Herun,Hu, Rongjiang,He, Zhiyong,Wei, Xianglun,Ma, Peng,Gan, Zaiguo,Yang, Chunli,Zhang, Hongbin,Chen, Liang,Qiu, Tianli Korean Nuclear Society 2021 Nuclear Engineering and Technology Vol.53 No.6

We developed a Dual-PPACs detector for fast neutron measurements that consists of two sets of PPAC: conventional PPAC and fission PPAC. A²³⁸U(U₃O₈) coating is placed in the fission PPAC's anode, which is used as the neutrons conversion layer. An experiment was performed to measure neutron time-of-flight (TOF) in which ²⁵²Cf spontaneous fission source was used. An excellent time resolution of 164ps has been observed at 6 mbar in isobutene gas. With the excellent time resolution of Dual-PPACs detector, exact neutron energy can be extracted from the timing measurement. The experimental detection efficiency was 1.9 × 10^-7, consistent with the efficiency of 2.5 × 10^-7 given by a Geant4 simulation. Ultimately, the results show that the Dual-PPACs detector is a suitable candidate for measuring fast neutrons in the future CiADS system.