http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
( Go Takayama ),( Shingo Yokota ),( Tetsuo Kondo ) 한국목재공학회 2021 한국목재공학회 학술발표논문집 Vol.2021 No.1
Nanocelluloses, which are produced by nano-downsizing processes of pulp fibers, have attracted much attention in recent years due to their excellent physical properties and specific surface characteristics. For material design using nanocelluloses, facile understanding of the relationship between structure and property is an essential issue. In contrast, as the 3D-stractural formation starting from biosynthesis of native nanocelluloses has been still complexed as a bottom-up mechanism, it prevents the control of their hierarchical structure in native systems including bacterial producing system. A model system of cellulose fiber spinning mechanism would contribute to the further understanding of the 3D-hierachical structure formation of nanocelluloses. Biosynthesis of native cellulose accompanied with polymerization and crystallization processes is well known to be achieved through synthetic enzyme complexes called Terminal Complexes (TCs). However, the following process including assembly of the nanofibrils secreted from cells has been still unclear, even though the combination of the biosynthesis and the following assembly process is of importance to establish the nano/micro fibrils of cellulose. Then, this unique process leads to the remarkable characteristics of native cellulose fibers. A cellulose-producing bacterium, Gluconacetobacter, has been extensively studied as a model-organism of the cellulose biosynthesis and there have been many studies on in vitro reconstruction of the cellulose synthesis activity. In this study, for the above goal, we first explored a method to extract intact TCs of Gluconacetobacter xylinus from the cell without outer cell walls, and then cellulose fiber spinning activity of the extracted TCs were examined to confirm the intact ability. Namely, bacteria (ATCC23769) were subjected to cell wall digestion treatment and then were burst by osmotic shock to obtain cell membrane fragments holding intact TCs. Finally, cellulose synthesis reaction was conducted using the burst cells.