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RISS 인기검색어
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- 저자 : ( Ali A. Moosavi Movabedi ) (검색결과 1 건)
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Kinetics of Denaturation of Human and Chicken Hemoglobins in the Presence of Co-solvents
( Davood Ajloo ),( Ali A. Moosavi Movabedi ) 생화학분자생물학회 2003 BMB Reports Vol.36 No.4
The stability of four hemoglobins (Hb) in dimer forms (low concentration) were investigated by the kinetics of denaturation. The rate constants of denaturation were obtained by variation of 280 nm absorption versus time in 10 mM Tris-HCI, 10 mM EDTA, pH 8.0 at 45℃ in the absence and presence of 0.5 M ethanol, dimethyl sulfoxide (DMSO), formamide, and glycerol. The results show the trend of rate constants in different co-solvents in the following order: chicken hemolysate < human hemolysate and chicken Hb D < chicken HbA. The buried surface area was calculated for Hb samples in the absence of co-solvents. Accordingly, the trend points out that: chicken Hb D > chicken Hb A > human Hb A. These results suggest that both chicken hemolysate and chicken Hb D are relatively more stable than human and chicken Hb A, respectively. However, the denaturation rate constants of Hb in different co-solvents have designated the following order: ethanol>DMSO>formamide>glycerol As a matter of fact, this phenomenon is an indication of an increase in the denaturation capacity (DC) and hydrophobicity, and a decrease in the surface tension of the solution in the preceding co-solvents.
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