For the purpose of investigate the immunoadjuvant effects of Bohuhtang, Bohuhtang was pretreated to Balb/c mice for 10 days, with intraperitoneally. After 10 days, haptenic antigen (VCR-BSA, vincristine- bovine serum albumin) The immunoadjuvant were f...
For the purpose of investigate the immunoadjuvant effects of Bohuhtang, Bohuhtang was pretreated to Balb/c mice for 10 days, with intraperitoneally. After 10 days, haptenic antigen (VCR-BSA, vincristine- bovine serum albumin) The immunoadjuvant were followed after 7 days of first immunization, complexed with Freund's complete adjuvant was immunized. The immunoadjuvant experiments were as follows:
1. The antibody production in Balb/c mouse immunized with VCR-BSA was remarkably increased in the pretreated group of Bohuhtang compared with saline treated(control) group.
2. The macrophage numbers in Balb/c mouse immunized with VCR-BSA was considerably increased about 2.9 times in the pretreated groups of Bohuhtang compared with control group.
3. The lysozyme activity of macrophage in Balb/c mouse that was immunized with VCR-BSA was increased gradually in the pretreated groups of Bohuhtang compared with control group.
4. In the Bohuhtang pretreated group, B cell(spleen cell) proliferation was increased with a dose dependent manner. At 20 ㎕/well of Bohuhtang, B cell proliferation activity was most highest.
5. On the other hand, in the T cell(thymocyte) proliferation Bohuhtang pretreated group had less considerable activity than in the case of B cell.
These results may suggest that Bohuhtang will have irnrnunoadjuvant effects on the cells which concerned with immuno mechanism.