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- 발행기관 : American Association of Endodontists] (검색결과 2 건)
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Proteome Expression in Human Periodontal Ligament after Delayed Hypothermic Preservation
Cho, S.Y.,Park, J.,Chung, W.Y.,Kim, E.,Jung, I.Y.,Choi, S.H.,Park, K.K.,Lee, S.J. American Association of Endodontists] 2017 JOURNAL OF ENDODONTICS - Vol.43 No.8
Introduction: Previous occasional successes after delayed replantation suggest that the presence of viable cells may not be the only factor for successful periodontal regeneration in delayed replantation. Various other factors such as proteins or the extracellular matrix (ECM) may play a role in this process. The purpose of this study was to characterize changes in the proteome components of periodontal ligament (PDL) tissue after hypothermic preservation of the tooth. Methods: Extracted teeth were divided into 4 groups: immediate sampling, sampling after 1 week of preservation at 4<SUP>o</SUP>C, sampling after 2 weeks of preservation at 4<SUP>o</SUP>C, and sampling after 1 week of dry storage at room temperature as a negative control. PDL tissues were collected from the root and stored immediately in liquid nitrogen. The tissues were subjected to 2-dimensional gel electrophoresis, and spot selection was executed. Selected spots that maintained the protein volume were then processed with matrix-assisted laser desorption and ionization time-of-flight mass spectrometry to identify the nature of the proteins. Results: Thirty-five selected spots were analyzed. Sixteen spots were identified as vimentin, and 3 spots were type VI collagen. The size of the 16 vimentin spots decreased gradually with increasing storage time, from 0 to 2 weeks, and decreased rapidly after dry storage. However, only the dry storage group differed significantly from the immediately sampled group. Conclusions: Vimentin was the most prominent protein followed by type VI collagen in volumetrically maintained protein spots. Although these proteins are known to be closely related with ECM integrity, the role of these proteins in delayed replantation is beyond the scope of this study. Further studies are needed to elucidate the possible role of these proteins for periodontal healing of delayed replantation.
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Ha, J.H.,Kwak, S.W.,Sigurdsson, A.,Chang, S.W.,Kim, S.K.,Kim, H.C. American Association of Endodontists] 2017 JOURNAL OF ENDODONTICS - Vol.43 No.10
Introduction: The aim of this study was to evaluate the effect of different pecking depth on the stress generated by the screw-in forces of a rotating endodontic file in simulated canals. Methods: Twenty simulated resin blocks with a J-shaped curvature were used. Twenty OneG files (MicroMega, Besancon, France) were assigned for a screw-in test depending on the pecking depth in 2 groups (n = 10). The files were operated at 300 rpm, and the up and down speed was controlled at 1 mm/s stroke velocity and a 10-millisecond dwell time using a customized device. The distances (pecking depth) for the pecking motion were 2 mm or 4 mm for each group; ''6 mm forward and 4 mm backward'' and ''6 mm forward and 2 mm backward'' movements were applied, respectively, for the 2 pecking groups. During the operation, the positive and negative apical loads were recorded at a rate of 50 Hz using customized software attached to the device. The maximum negative apical load (screw-in force [SF]) was recorded, and the total energy during pecking motion until the file reached the working length (cumulative screw-in forces [CSFs]) was computed. The data were analyzed using an independent t test at a significance level of 95%. Results: No significant difference in SF was found between the 2 groups of pecking depths. However, the longer pecking depth (4-mm group) showed a significantly larger CSF compared with the shorter pecking depth group (P < .05). Conclusions: The shorter pecking depth may generate lower overall stresses for the root dentin as well as the instrument.
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