http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
- 中文 을 입력하시려면 zhongwen을 입력하시고 space를누르시면됩니다.
- 北京 을 입력하시려면 beijing을 입력하시고 space를 누르시면 됩니다.
RISS 인기검색어
- 검색키워드
- 저자 : Zhu Youshuang (검색결과 1 건)
- 무료
- 기관 내 무료
- 유료
-
Zhu Youshuang,Zhang Haibo,Cao Mingle,Wei Zhenzhen,Huang Feng,Gao Peiji 한국생물공학회 2011 Biotechnology and Bioprocess Engineering Vol.16 No.5
Production of laccase using a submerged culture of Trametes versicolor sdu-4 was optimized using a central composite design of the Response Surface Methodology. Optimized conditions gave a laccase yield of 4,213 U/L which was approximately three times of that in basal medium. The laccase was purified to homogeneity using a three-step process. The overall yield of the purification was 58%, with a purification fold of 11.4 and a specific activity of 1320.7 U/mg protein. The molecular mass of the laccase was 60 kDa. The optimum pH values of the enzyme were 2.2, 3.7, and 7 for the oxidations of ABTS, DMP, and syringaldazine, respectively. The enzyme had adaptability to a broad pH range and high temperature and wsa stable at pH 3.0 ~ 10.0. The half-life of this laccase at 70°C was 2.2 h. Methyl red, 2-bromophenol, and 4-bromophenol were oxidized by the purified laccase in the absence of mediators. Purified laccase was effective in the decolorization of several dyes and was not inhibited by Cu^(2+), Mn^(2+), Zn^(2+), Na^+, K^+, Mg^(2+), Ba^(2+), and Ca^(2+) at 5 mM. These excellent characteristics made it a highly attractive candidate for industrial use.
연관 검색어 추천
이 검색어로 많이 본 자료
활용도 높은 자료
