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Yinhui Wang,Yanmei Gao,Zhixiang Zhou,Dong Liu 국제구조공학회 2016 Steel and Composite Structures, An International J Vol.21 No.5
To avoid the cracks of cast-in-place concrete in shear pockets and seams in the traditional composite beam with precast decks, this paper proposed a new type of prefabricated steel truss-concrete composite beam (ab. PSTC beam) with pre-embedded shear studs (ab. PSS connector). To study the initial cracking load of concrete deck, the development and distribution laws of the cracks, 3 PSTC beams were tested under hogging moment. And the crack behavior of the deck was compared with traditional precast composite beam, which was assembled by shear pockets and cast-in-place joints. Results show that: (i) the initial crack appears on the deck, thus avoid the appearance of the cracks in the traditional shear pockets; (ii) the crack of the seam appears later than that of the deck, which verifies the reliability of epoxy cement mortar seam, thus solves the complex structure and easily crack behavior of the traditional cast-in-place joints; (iii) the development and the distribution laws of the cracks in PSTC beam are different from the conventional composite beam. Therefore, in the deduction of crack calculation theory, all the above factors should be considered.
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Kuiling Zu,Jianxia Li,Shubin Dong,Yunyu Zhao,Shenjian Xu,Zhixiang Zhang,Liangcheng Zhao 한국유전학회 2017 Genes & Genomics Vol.39 No.6
As a specialized seed appendage, the aril is a noteworthy and taxonomically important feature of Celastrus orbiculatus; the aril also has important ornamental value and biological functions. To better understand the mechanism of aril development, paraffin sections were used to examine its morphogenesis, and RNA-sequencing (RNA-seq) technology was employed for transcriptional profiling of the developing aril. Our results revealed that the aril of C. orbiculatus was formed by the cells of the exostomal region of the outer integument. By analyzing global changes in the transcriptome, 41,560,806, 42,789,340, and 44,496,748 clean reads from three cDNA libraries were generated and assembled into 87,600 unigenes with N50 of 1328 bp, in which 31,971 (36.49%) were annotated to Nr database. Furthermore, a total of 25,914 (29.79%), 14,394 (16.4%) and 11,957 (13.65%) genes were successfully annotated with hierarchical 48 GO terms, 26 KOGs and 32 KEGG pathways. Totally, 104, 1887 and 1967 differently expressed genes were identified in CO-F2 versus CO-F3, CO-F2 versus CO-YF1 and CO-F3 versus CO-YF1, respectively. GO and KEGG pathway analyses of DEGs involved in cell–cell communication and plant hormone signal transduction contributed to aril development were identified. Furthermore, we found putative key target genes of WRKY, Aux/IAA, ARF and MADS-box family’s transcription factors related to aril development. Real-time qPCR was performed on eight genes randomly selected from important transcription factors to validate the expression profiles obtained by RNA-seq. This work provides a platform for future genetic and functional genomics research on the molecular mechanisms of aril structure, and expands our understanding of the molecular mechanism and evolutionary analysis of aril development in other members of the Celastraceae.
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Shengjia Huang,Xinya Liu,Bo Xiong,Xia Qiu,Guochao Sun,Xiaojia Wang,Xu Zhang,Zhixiang Dong,Zhihui Wang 한국식품과학회 2019 Food Science and Biotechnology Vol.28 No.3
The nomilin and limonin content in citrus fruitsof different varieties was determined at fruit growth andmaturation stages by HPLC. The results showed that thetwo limonoids can be separated, identified, and quantifiedin citrus fruits within 10 min by the developed method. Themethod exhibited good precision, repeatability, stability,and recovery rate. The content of limonin and nomilin inmost citrus fruits presented an increasing trend initially,and then decreased during fruit growth and maturation; apeak was observed at the young fruit or fruit expansionstage. The dropped fruits also contained some amount oflimonoids, suggesting their industrial application. Thevariation and cluster analyses results revealed that theorange varieties contained the highest amount of limonoidsat the mature stage. The results of this study will enablebetter use of citrus limonoids.
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Ruan Xinmin,Du Hongyang,Zhan Xinchun,Cong Xihan,Shi Fuzhi,Li Juan,Luo Zhixiang,Dong Zhaorong 한국식물생명공학회 2023 Plant biotechnology reports Vol.17 No.1
Developing genotypes with high-nitrogen use efficiency is a prerequisite for achieving sustainable agriculture and high yields. In this study, both physiological and metabolic analyses were performed to evaluate the NUE (nitrogen use efficiency) of two elite indica rice cultivars: OM052 and Huanghuazhan (HHZ). The pNUE and gNUE of OM052 were higher than HHZ under three N conditions, and significant (p < 0.05) especially under both LN and MN conditions. The contents of soluble sugar and malate were significantly different between OM052 and HHZ under LN and HN conditions. The GDH activity in OM052 was significantly high than that in HHZ under HN condition. Metabolic analyses showed that 136 and 142 differentially accumulating metabolites were identified between OM052 and HHZ under LN and HN conditions, respectively. KEGG analysis showed that ‘aminoacyl-tRNA biosynthesis’ was also the most significant enriched in LN_OM052 vs. LN_ HHZ and ‘Glyoxylate and dicarboxylate metabolism’ and ‘citrate cycle’ were the most significantly enriched in HN_OM052 vs. HN_ HHZ. The contents of four metabolites (cis-aconitate, isocitrate, fumarate, and malate) involved in the TCA cycle were significantly upregulated in HN condition compared with LN condition, regardless of OM052 and HHZ. Taken together, the results of our study revealed the physiological and metabolic differences underlying the OM052 and HHZ and provide new insights into coordinating C and N metabolism and improving the NUE of rice.
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