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        Disinfection Effect and Its Mechanism of Electrolyzed Oxidizing Water on Spores of Bacillus subtilis var. niger

        Wenwei Tang,Xinping Zeng,Yusheng Zhao,Guoqing Ye,Wenchi Gui,Yaming Ni 한국식품과학회 2011 Food Science and Biotechnology Vol.20 No.4

        A study was carried out on the disinfection efficiency of electrolyzed oxidizing water (EOW) on spores of Bacillus subtilis var. niger. The results showed a remarkable fungicidal rate of 100% after 20 min duration of 191mg/L active available chlorine (ACC). The disinfection effect was improved with increased ACC or prolonged disinfection time, while organic interferents exerted a strong concentration-dependent inhibition against the disinfection. The disinfection mechanism was also investigated at bio-molecular level. EOW decreased dehydrogenase activity, intensified membrane permeability,elevated suspension conductivity, and caused leakage of intracellular K+, proteins, and DNA, indicating a damage of cell walls and membranes. Effects of EOW on microbiological ultra-structures were also verified by transmission electronic microscopy (TEM) images,showing that EOW destroyed protective barriers of the microbe and imposed some damages upon the nucleus area.

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