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Zhang Yan,Liang Zhuo,Jiang Mulan,Wan Xia,Gon Yangmin,Zhang Yinbo,Huang Fenghong 한국식품과학회 2013 Food Science and Biotechnology Vol.22 No.2
In modern diet of human being, the ω-6/ω-3ratio of dietary fatty acid was shifted much towards ω-6series. In this work, a fatty acid desaturase (FAD) gene lkfad15 was cloned and identified from oleaginous yeast Lipomyces kononenkoae. Function analysis results shown that LKFAD15 is a novel Δ12/Δ15 bifunctional FAD which could not only produce linoleic acid and α-linolenic acid use just oleic acid as substrate but also adjust the ω-6/ω-3 fatty acids ratio to the WHO recommended ratio. Phylogenetic analysis of LKFAD15 suggested it is a specific intermediate product of gene evolution derives from independent gene duplication events.
Xia Wan,Yinbo Zhang,Ping Wang,Mulan Jiang 한국미생물학회 2011 The journal of microbiology Vol.49 No.1
The delta 6-desaturase gene was cloned from Rhizopus stolonifer, which could accumulate up to 49% of gamma-linolenic acid (GLA, C18:3 Δ^(6,9,12)) to the total fatty acids. The cloned DNA contains a 1,380 bp open reading frame encoding a protein of 460 amino acids, which showed high similarity to those of fungal delta 6-desaturases with three conserved histidine-rich motifs and HPGG motif. Notably, this deduced sequence had a shorter C-terminus. Results demonstrated that the cDNA sequence exhibited delta 6-desaturase activity by accumulation of about 22.4% of GLA to the total fatty acids in the recombinant Pichia pastoris strain GS115.
서원명 ( Suh Won Myung ),윤용철 ( Yoon Yong Cheol ),김태균 ( Kim Tae Kyun ),최만권 ( Choi Man Gwon ),장은파 ( Zhang Yinbo ) 한국농공학회 2007 한국농공학회 학술대회초록집 Vol.2007 No.-
This research was carried out to investigate and analyze the waste amounts and treatment status fot pig farms located around Goseong-Gun regions in which comparatively large scale pig industries are clustered. And T-P, T-N, and various contents of noxious heavy metals, assumed to be accumulated in farm drainage systems near livestock waste composting center, were measured in pursuit of the seasonal variations. So far no public composting center was found in this regions. But most of individual livestock farms own self treatment facilities for animal wastes. Some amounts of livestock wastes, especially those collected from farms equipped with insufficient capacity of waste composting facilities, are known to be casted to deep ocean.