The Flower Extract of Abelmoschus manihot (Linn.) Increases Cyclin D1 Expression and Activates Cell Proliferation

( Yea-in Park ),( Yeo-eun Cha ),( Minsu Jang ),( Rackhyun Park ),( Sim Namkoong ),( Jongbock Kwak ),( Ik-soon Jang ),( Junsoo Park ) 한국미생물 · 생명공학회 2020 Journal of microbiology and biotechnology Vol.30 No.7

Abelmoschus manihot (Linn.) is a medicinal herbal plant that is commonly used to treat chronic kidney disease and hepatitis. However, its effect on cell proliferation has not been clearly revealed. In this report, we sought to determine the effect of the flower extract of A. manihot (FA) on cell proliferation. Based on our findings, FA increased the proliferation of human diploid fibroblast (HDF) and HEK293 cells. Through cell cycle analysis, FA was found to increase the number of HDF cells in the S phase and G2/M phase. FA also increased the expression of cyclin D1 and enhanced the migration of HDF cells. By administering FA to HDF cells with ≥30 passages, a decrease in the number of senescence-associated β galactosidase-positive cells was observed, thereby indicating that FA can ameliorate cellular senescence. Collectively, our findings indicate that FA increases cyclin D1 expression and regulates cell proliferation.

Viral Etiology of Acute Exacerbations of Bronchiectasis: A Retrospective Single-center Study

( Yea Eun Park ),( Heungsup Sung ),( Yeon-mok Oh ) 대한결핵 및 호흡기학회 2020 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.128 No.-

Purpose Bacterial infections are well known factors underlying acute exacerbations in bronchiectasis. However, virus infections may also contribute to acute exacerbations. We aimed to assess the rate of viral infection in acute exacerbation of bronchiectasis and the associated clinical factors. Method We retrospectively reviewed the electronic medical records of patients who presented to the emergency department or the respiratory care inpatient unit at a tertiary referral center in South Korea (2015-2019) and underwent diagnostic testing for bacterial and viral pathogens. All patients were diagnosed with bronchiectasis by chest computerized tomography and were prescribed antibiotics for minimum 3 days for managing the deteriorating symptoms. Result Viral pathogens were detected in 204 of 801 patients enrolled in our study (25.5%). The most common viral pathogen isolated was influenza virus A (24.5%), followed by rhinovirus (22.7%), influenza virus B (10.2%), respiratory syncytial virus B (8.8%), and human metapneumovirus (6.0%). In a total of 148 patients, viral but not bacterial pathogen was detected, while no pathogens were found in 451 exacerbated patients. Multivariable analysis revealed that female sex (adjusted OR, 1.608; 95% CI, 1.094- 2.363; P = 0.016), chronic heart disease as a comorbidity (adjusted OR, 1.723; 95% CI, 1.096-2.708; P = 0.018) were positively associated with viral etiology of acute exacerbation in bronchiectasis patients, while the presence of radiographic infiltration (adjusted OR, 0.612; 95% CI, 0.395-0.948; P = 0.028) was negatively associated. Conclusion Respiratory viruses were identified in approximately 25% of the acute exacerbations observed among patients with bronchiectasis; influenza and rhinovirus were detected in >50% cases. More attention to viruses as a causative pathogen for acute deteriorating symptoms in patients with bronchiectasis is warranted.

Respiratory Viruses in Acute Exacerbations of Bronchiectasis

Park Yea Eun,Sung Heungsup,Oh Yeon-Mok 대한의학회 2021 Journal of Korean medical science Vol.36 No.34

Background: Bacterial infections are well known factors underlying acute exacerbations in bronchiectasis. However, viral infections may also contribute to acute exacerbations. We aimed to assess the rate of viral detection in acute exacerbations of bronchiectasis, and the associated clinical factors. Methods: Diagnostic tests for viral and bacterial etiologies were performed in 792 patients with bronchiectasis who visited the emergency room or the respiratory care inpatient unit in a tertiary referral center in South Korea. All patients were diagnosed with bronchiectasis by chest computerized tomography and were prescribed antibiotics for a minimum of 3 days. Results: Viral pathogens were detected in 202 of the 792 enrolled patients (25.5%). The most common viral pathogen isolated was influenza A virus (24.8%), followed by rhinovirus (22.4%), influenza B virus (9.8%), respiratory syncytial virus B (8.9%), and human metapneumovirus (6.1%). In 145 patients, a viral, but not bacterial, pathogen was detected, whereas no pathogens were found in 443 patients with exacerbations. Multivariable analysis revealed that female sex and chronic heart disease as a comorbidity were positively associated with viral detection in acute exacerbations of patients with bronchiectasis, whereas the presence of radiographic infiltration was negatively associated. Conclusion: Respiratory viruses were identified in approximately 25% of the acute exacerbations observed among patients with bronchiectasis. Of the viruses detected, influenza viruses and rhinovirus made up over 50%. More attention to viruses as possible causative pathogens for acute deteriorating symptoms in patients with bronchiectasis is warranted.

용매 첨가제의 용해도 계수가 공액고분자의 자기조립 거동에 미치는 영향

권은혜 ( Eun Hye Kwon ),이정익 ( Jeong Ik Lee ),박소영 ( So Young Park ),함예은 ( Yea Eun Hahm ),박영돈 ( Yeong Don Park ) 한국공업화학회 2020 공업화학전망 Vol.23 No.5

유기전자소자는 용액공정을 통한 대량생산이 가능하기 때문에 기존 무기전자소자에 비해 제조비용이 저렴하고 대면적 생산이 가능하며, 유기분자의 본연 특징으로 인해 유연하고 가벼운 소자를 구현할 수 있다. 그러나 무기반도체에 비하여 현저히 낮은 전하이동도 특성은 유기전자소자의 상용화에 걸림돌이 되고 있다. 따라서 공액고분자의 결정화도, 모폴로지, 분자배향 최적화를 통한 자기조립 박막 제조는 전하이동을 원활히 하기 때문에 유기전자소자의 개발에 필수적이다. 본 기고에서는 유기전자소자의 활성층으로 사용되는 공액고분자의 자기조립을 유도하기 위한 다양한 특성을 갖는 용매 첨가제의 효과에 대해서 알아보고, 특히 첨가제의 용해도 계수가 공액고분자의 자기조립 거동에 미치는 영향에 대해 자세히 논의하고자 한다.

뉴욕시 액티브 디자인 가이드라인을 통한 국내 도시공원 사례 연구

박예은(Park, Yea-Eun),박진희(Park, Jin-Hee),황연숙(Hwang, Yeon-Sook) 한국실내디자인학회 2022 한국실내디자인학회 학술대회논문집 Vol.24 No.1

재취업 중고령 임금근로자의 좋은 일자리 진입 영향요인 분석

박예은 ( Yea Eun Park ),정영순 ( Young Soon Chung ) 한국사회복지정책학회 2016 사회복지정책 Vol.43 No.1

본 연구에서는 재취업한 임금근로 중고령자의 좋은 일자리 진입가능성에 영향을 미치는 요인을 로지스틱 회귀분석을 사용하여 분석하였다. 분석결과, 임금이 중위임금의 2/3를 초과하고 상용직이면서 전일제인 좋은 일자리로 진입한 비율은 21.6%뿐인 것으로 나타났다. 개인적 특성 요인에서는 공적이전소득이 높을수록 자산소득이 작을수록, 인적자본 특성 요인에서는 초졸에 비해 전문대졸 이상이고 종전 종사상지위가 비정규직에 비해 정규직일수록, 구직 특성 요인에서는 노동시장진입까지 구직기간이 짧을수록, 일자리 특성 요인에서는 농림어업 및 단순노무직에 비해 관리ㆍ전문ㆍ사무직, 기능직 및 기술직, 서비스 및 판매직일수록, 5인 미만 사업장에 비해 300-999인 사업장일수록 좋은 일자리 진입가능성이 높은 것으로 확인되었다. 이러한 연구결과는 중고령자의 좋은 일자리 진입가능성을 높이기 위해 이직 이후보다 이직 예정일 때부터 취업지원서비스가 제공되는 것이 중요하고, 취업지원 시 직종, 사업장 규모를 고려하여 좋은일자리일 가능성이 높은 일자리로 연계시키는 것이 효과적임을 시사해주고 있다. This study identified factors influencing the entry of middle and old aged workers into good jobs, by using the logistic regression analysis. The results showed that only 21.6% of middle and old aged workers secured good jobs which are defined as full-time, and regular positions with more than 2/3 of the median wage. In personal characteristics, people with higher public transfer income and smaller assets income had a significantly higher likelihood of getting a good job. In human capital characteristics, two-year college graduates had an increased possibility of getting a good job than elementary school graduates. Moreover, people who previously had regular jobs had a higher likelihood of getting a good job than people who previously had irregular jobs. In current job characteristics, people engaged in management/ professional/clerical work, technical work, and sales/services had a higher likelihood of getting a better job than people engaged in agriculture/forestry/fishery and simple labor. Also people worked at companies with 300-999 employees had a higher likelihood of getting a better job than people worked at companies with less than 5 employees. The study suggested that it is important to provide employment support services before changing a job to improve the likelihood of getting a good job. Furthermore, it revealed that employment support services should support people to consider type of occupation and business size which increase the likelihood of getting a good job.

시간빈곤이 일가족양립 만족도에 미치는 영향

박예은(Park, Yea eun),윤미(Yoon, Mi),이예솔(Lee, Yeasol),이효진(Lee, Hyojin),정익중(Chung, Ick Joong) 경성대학교 사회과학연구소 2016 社會科學硏究 Vol.32 No.2

기혼취업 여성들은 가정과 직장에서의 이중 역할을 감당하게 되며 극심한 시간부족에 시달리고 있다. 가사 및 돌봄노동, 유급노동 등으로 인해 여가시간을 박탈당함은 물론이고 식사, 수면 등 기본적인 생활에 필요한 시간까지 줄여야 하는 시간빈곤을 경험하게 된다. 본 연구에서는 시간이 개인의 삶의 질에 중대한 영향을 미친다는 관점에 기반하여 기혼취업여성이 일과 가족을 양립하는데 시간빈곤이 실제적으로 영향을 미치는지 분석하였다. 이를 위해 여성가족패널(KLoWF)의 4차년도 자료가 활용되었으며, 그 중 일가족양립의 어려움을 보다 심각하게 겪는 시기인 미취학 자녀를 둔 기혼취업여성 400명의 자료에 대해 회귀분석을 실시하였다. 그 결과 시간빈곤 수준이 심화될수록 일가족양립 만족도가 낮아지는 것으로 나타났다. 이에 차후 관련 정책을 수립함에 있어서 장시간 근로문화 개선, 근무시간의 유연성 강화 등 ‘시간빈곤’ 개념을 반영한 접근이 요구됨을 제언하였다. As married working women in Korea take on double roles in the contexts of family and the workplace, they suffer from severe time poverty. Between housework, caretaking, and paid work hours, not only are they deprived of personal leisure time, but they also experience time poverty with regard to eating, sleeping, and other basic life activities. Based on the standpoint that time exerts a significant impact on personal quality of life, this study seeks to analyze the effect of time poverty on working mothers who are engaged in the balancing act of work-family reconciliation. Utilizing the fourth wave of Korean Longitudinal Survey of Women and Families (KLoWF), we performed regression analysis on a sample of 400 working women, whose current status as mothers of preschool children posed great potential difficulty in terms of work-family reconciliation. As a result, the more deepening time poverty level showed to result in lower work-family reconciliation satisfaction. This study also suggests the need for the establishment of relevant policies that reflect improving workplace cultures related to long working hours and enhancing schedule flexibility at work, based upon this concept of ‘time poverty’.

Papaverine Exerts Neuroprotective Effect by Inhibiting NLRP3 Inflammasome Activation in an MPTP-Induced Microglial Priming Mouse Model Challenged with LPS

( Yea-hyun Leem ),( Jin-sun Park ),( Jung-eun Park ),( Do-yeon Kim ),( Hee-sun Kim ) 한국응용약물학회 2021 Biomolecules & Therapeutics(구 응용약물학회지) Vol.29 No.3

Microglial priming is the process of microglial proliferation and activation in response to neurodegeneration and abnormal protein accumulation. Priming makes microglia susceptible to secondary inflammatory stimuli and causes exaggerated inflammatory responses. In the present study, we established a microglial priming model in mice by administering a single injection of 1-methyl- 4-phenyl-1,2,3,6-tetrahydropyridine (MPTP, 20 mg/kg). MPTP induced microglial activation without dopaminergic degeneration; however, subsequent treatment with a sub-toxic dose of lipopolysaccharides (LPS) induced an amplified inflammatory response and caused nigrostriatal dopaminergic degeneration. These pathological and inflammatory changes, including microglial activation and dopaminergic cell loss in the substantia nigra (SN) area were reversed by papaverine (PAP) administration. In addition, MPTP/LPS enhanced interleukin-1β (IL-1β) expression and processing via nod-like receptor protein 3 (NLRP3) inflammasome activation in the SN region of mice. However, PAP treatment suppressed inflammasome activation and subsequent IL-1β maturation. Moreover, PAP inhibited nuclear factor-κB (NF-κB) and enhanced cAMP-response element binding protein (CREB) activity in the SN of MPTP/LPS mice. These results suggest that PAP inhibits the activation of NLRP3 inflammasome by modulating NF-κB and CREB signaling pathways, which results in reduced microglial activation and neuronal cell death. Thus, PAP may be a potential candidate for the treatment of Parkinsons’s disease, which is aggravated by systemic inflammation.

The Dose-response Effects of Nano-encapsulated Conjugated Linoleic Acid on in vitro Ruminal Fermentation Characteristics

Eun Tae Kim,Wan Heo,Ha Yeon Jeong,Sang Bum Kim,Beom Young Park,Ji Hoo Park,Ho Baek Yoon,Sung Sill Lee,Yea Hwang Moon,Young Dae Ahn,Young Jun Kim 경상대학교 농업생명과학연구원 2017 농업생명과학연구 Vol.51 No.4

This study was aimed to evaluate the dose-response the effects of nano-encapsulated conjugated linoleic acids(CLAs) on in vitro ruminal fermentation profiles. A fistulated Holstein cow was used as a donor of rumen fluid. Nano-encapsulated CLAs(LF, 5% of nano-encapsulated CLA-FFA; HF, 10% of nano-encapsulated CLA-FFA; LT, 5% of nano-encapsulated CLA-TG; HT, 10% of nano-encapsulated CLA-TG) were added to the in vitro ruminal fermentation experiment. In the in vitro ruminal incubation test, the total gas production on incubation with nano-encapsulated CLAs was increased significantly according to the incubation time, compared with the control(p<0.05). The tVFA concentrations on addition of LF and HT were significantly higher than that of the control(p<0.05). Thus, nano-encapsulated CLAs might improve the ruminal fermentation characteristics without adverse effects on the incubation process. In addition, the population of Butyrivibrio fibrisolvens which is closely related to ruminal biohydrogenation was increased by adding HT, while decreased by adding LF at 12 h incubation. These results showed that nano-encapsulated CLA-FFA could be applied to enhance CLA levels in ruminants by maintaining the stability of CLA without causing adverse effects on ruminal fermentation profiles considering the optimal dosage.

Effects of dextrinization and octenylsuccinylation of high amylose starch on complex formation with ω-3 fatty acids (EPA/DHA)

Park, Eun Young,Choi, So Mang,Lim, Seung-Taik,Kim, Jong-Yea Elsevier 2018 Food hydrocolloids Vol.77 No.-

<P><B>Abstract</B></P> <P>High amylose maize starch (70% amylose) was dextrinized in an acid/alcohol solution (0.36% HCl in ethanol, 50 °C for 24 h), and the starch and its dextrin were octenylsuccinylated (DS 0.019) to be utilized as complex forming agents for ω-3 fatty acids (eicosapentaenoic acid and docosahexaenoic acid). A fish oil was used as the fatty acid sample and dispersion with starch and fish oil was stirred at 60 °C for 3 h and cooled at an ambient temperature for 3 h. The complex was isolated as the precipitates after centrifugation (25,000 × g, 25 °C for 30 min), and then the recovery of fatty acids in the complex and crystalline characteristics of the complex were examined. Dextrinization improved the efficiency of complex formation, increasing the recovery of fatty acids and decreasing the recovery of solid precipitates. Octenylsuccinylation, however, retarded the complex formation showing a decrease in fatty acid recovery. It was hypothesized that intermolecular complex between amylose and octenyl group in the substituents was formed during the reaction because the modified starch and dextrin without fatty acids showed a new endotherm (at around 100 °C) in their thermograms. Because of the possible competition between the substituents and fatty acids in complex formation, octenylsuccinylation hindered the complex formation between amylose and fatty acids. The fatty acid complex particles prepared with native starch or dextrin showed average particle size less than 400 nm with zeta potential below −40 mA, assuring the dispersion stability during an ambient storage.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Dextrinized and/or OSA modified starch was used for complex formation with fish oil. </LI> <LI> Dextrin was efficient in complex formation showing a high EPA/DHA and solid ratio. </LI> <LI> Dextrinization improved dispersion stability of the complex particles. </LI> <LI> Octenylsuccinylation retarded the complex formation showing a low EPA/DHA recovery. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>