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Ma, Shi-Xun,Kim, Hyoung-Chun,Lee, Seok-Yong,Jang, Choon-Gon Elsevier 2018 Neurochemistry International Vol.121 No.-
<P><B>Abstract</B></P> <P>Opioid addiction is a growing problem for public health, and opioids have correspondingly become more heavily regulated over time. We have previously shown that TRPV1 plays a critical role in morphine addiction using a self-administration paradigm in rats, and the current study evaluates the effects of the TRPV1 signaling pathway on morphine self-administration (SA). We found that treatment with a selective TRPV1 antagonist, SB366791, significantly decreased the morphine SA-induced activation of Ca2<SUP>+</SUP>/calmodulin-dependent protein kinase II (CaMKII), Akt and the cAMP response element binding protein (CREB) in the nucleus accumbens (NAc). In addition, phospho-PKA and phospho-PKC expression levels were significantly increased in the NAc of the morphine-SA groups, regardless of SB366791 treatment. Finally, local microinjection of SB366791 into the NAc significantly suppressed the maintenance of morphine SA. Taken together, our findings highlight that TRPV1 plays an important role in morphine addiction, likely via activation of the CaMKII-CREB pathway in the NAc.</P> <P><B>Highlights</B></P> <P> <UL> <LI> TRPV1 antagonist, SB366791, decreased the activation of CaMKII, Akt, and CREB induced by morphine SA. </LI> <LI> p-PKA and p-PKC expression levels were significantly increased in the NAc of the morphine-SA groups, regardless of SB366791 treatment. </LI> <LI> Local microinjection of SB366791 into the NAc suppressed the maintenance of morphine SA. </LI> </UL> </P>
A sheathless CE/ESI-MS interface with an ionophore membrane-packed electro-conduction channel
Shi, Lian-Hua,Jin, You-Xun,Moon, Dong-Cheul,Kim, Sook-Kyung,Park, Sang-Ryoul WILEY-VCH Verlag 2009 Electrophoresis Vol.30 No.10
<P>A robust and convenient sheathless CE/ESI-MS interface realized with an ionophore membrane-packed electro-conduction channel is described. Sheathless interfaces that may provide higher sensitivity for MS detection than sheath flow-supported interfaces generally show instability and short lifetimes due to their imperfection in making an electrical contact with the emitter tip. In this work, we designed a sheathless interface based on an ionophore membrane-packed electro-conduction channel. At the joining point of the CE capillary and the emitter capillary, the conduction channel was implemented toward the exterior of the interface body, where a platinum wire electrode was placed. The conduction channel transferred the electric field from the external Pt electrode to the joining point, but prevented the effluent of CE from leaking. The interface body was designed to have receptacles for standard capillary tubing with finger-tight fittings, which allowed easy replacement of capillary tubing. Stable electrospray was observed for an extended time period without any signs of bubbling or damage to the emitter tip. No significant increment of dead-volume at the interface was observed for well-aligned capillaries. Sensitive and stable CE-MS detection of the model compound of creatinine and uric acid was demonstrated.</P>
Shi-Ying Zhou,Gui-He Qin,Yang Xun,Yu-Bo Jin 보안공학연구지원센터 2008 International Journal of Security and Its Applicat Vol.2 No.3
Eelectronic device technology has been monopolized by binary system for many years. The circs not only makes it impossible to break through the existing technology bottleneck, but also brings a lot of potential safety problems. Aiming at this question, a new electronic communication technology is presented in this paper. The technology quantifies the time axis and the voltage axis synchronously, uses the quantified time dot as the address of the communication, and realizes the transmission of the multi-system [1] data via transmitting the multi-steps voltage quantification. The technology solves the bottleneck problem of the speed, circuit and electromagnetism in the electronic communication, changes the binary system coding mode and communication connection form of the electronic device, reduces the transmission quantity of the redundant information, advances the security of electronic system and network, debases the complexity of the devices connection, enhances the rate of the processing and the transmission, simplifies the transformation between the difference protocols. The experimental results approve the validity and the robustness of the technology.
Identification of Bovine Pregnancy-Specific Whey Proteins using Two-Dimensional Gel Electrophoresis
Rong Xun Han,Su Min Choi,Myung Youn Kim,Yan Shi Quan,Baek-Chul Kim,Yun Fei Diao,Reza Koqani,Chang Sik Park,Dong Il Jin 한국동물생명공학회(구 한국동물번식학회) 2008 Reproductive & developmental biology Vol.32 No.4
The early diagnosis of bovine pregnancy is an essential component of successful reproductive planning on farms, because lack of bovine pregnancy over the long term results in reproductive failure and low milk yield‐the latter of which is a special concern on dairy farms. This study was designed to identify early pregnancy‐specific whey proteins in bovine, by comparing milk samples collected from cattle during pregnancy (Days 30 and 50) and from non‐pregnant cattle. In this study, differentially expressed proteins in five pregnant and five non‐pregnant Holstein dairy cattle were investigated and compared, using proteomics analysis. The first dimension was applied to a pH 3.0~10.0 strip, by loading a 2‐mg milk protein sample. After the second‐dimension separation was performed, the gels were stained with colloidal Coomassie brilliant blue. The stained gels were scanned and the images were analyzed, to detect variations in protein spots between non‐pregnant and pregnant cattle milk protein spots, using ImageMaster; this was followed by analysis with MALDI TOF‐MS. Analysis of the 2‐DE gel image resulted in a total of approximately 500~600 protein spots, of which 12 spots were differentially expressed, six spots were up‐regulated, and four spots were downregulated; two spots were identified as pregnancy‐specific proteins. These proteins were identified as lactoferrin, NADH dehydrogenase subunit 2, albumin, serum albumin precursor and transferrin. Our results via 2‐D PAGE analysis revealed composite profiles of several milk proteins related to early bovine pregnancy, implying the possible use of these milk proteins in the early detection of bovine pregnancy.
Yan Shi Quan,Kenji Naruse,Baek Chul Kim,Hong Rye Kim,Rong Xun Han,Su Min Choi,Chang Sik Park,Dong Il Jin 한국동물생명공학회(구 한국동물번식학회) 2007 Reproductive & developmental biology Vol.31 No.4
Insulin, transferrin and selenium (ITS) complex is reported to improve in vitro development of oocytes and embryos. This study was carried out to investigate the effects of ITS during in vitro culture (IVC) of porcine parthenogenetic and nuclear transfer (NT) embryos on subsequent developmental capacity in vitro. The electrically activated oocytes were cultured in Porcine Zygote Medium (PZM-3) with various concentrations (0, 0.1, 0.5, and 1.0%) of ITS for 7 days. Also, the electrically activated reconstructed embryos were cultured in PZM-3 with various concentrations (0, 0.1, 0.5, and 1.0%) of ITS for 6 days. Addition of ITS to culture medium did not affect development of porcine parthenogenetic embryos in vitro. To test the effect of ITS on the in vitro development of porcine NT embryos, factorial experiments were also performed for in vitro maturation (IVM) medium (TCM-199) with or without 1% ITS and culture medium (PZM-3) with or without 0.5% ITS. Addition of 0.5% ITS to culture medium increased (p<0.05) the proportion of NT blastocysts compared with non-treated group. In contrast, addition of 1% ITS to culture medium was ineffective or had a detrimental effect. Also, addition of ITS only to maturation medium increased (p<0.05) the percentage of NT blastocysts formation compared with the control group. In conclusion, addition of ITS to IVM or IVC medium could improve subsequent blastocyst development of porcine NT embryos.
Yan Shi Quan,Kenji Naruse,Su Min Choi,Myung Youn Kim,Rong Xun Han,Chang Sik Park,Dong Il Jin 한국동물생명공학회(구 한국동물번식학회) 2008 Reproductive & developmental biology Vol.32 No.4
Interspecies somatic cell nuclear transfer (iSCNT) is a valuable tool for studying the interactions between an oocyte and somatic nucleus. The object of this study was to investigate the developmental competence of in vitro‐matured porcine oocytes after transfer of the somatic cell nuclei of 2 different species (goat and rabbit). Porcine cumulus oocytes were obtained from the follicles of ovaries and matured in TCM‐199. The reconstructed embryos were electrically fused with 2 DC pulses of 1.1 kV/cm for 30 μs in 0.3 M mannitol medium. The activated cloned embryos were cultured in porcine zygote medium‐3 (PZM‐3), mSOF or RDH medium for 7 days. The blastocyst formation rate of the embryos reconstructed from goat or rabbit fetal fibroblasts was significantly lower than that of the embryos reconstructed from porcine fetal fibroblast cells. However, a significantly higher number of embryos reconstructed from goat or rabbit fetal fibroblasts cultured in mSOF or RDH, respectively, developed to the morular stage than those cultured in PZM‐3. These results suggest that goat and bovine fetal fibroblasts were less efficacious than porcine‐porcine cloned embryos and that culture condition could be an important factor in iSCNT. The lower developmental potential of goat‐porcine and porcine‐bovine cloned embryos may be due to incompatibility between the porcine oocyte cytoplasm and goat and bovine somatic nuclei.
Simulation of 7050 Wrought Aluminum Alloy Wheel Die Forging and its Defects Analysis based on DEFORM
HUANG Shi-Quan,YI You-Ping,ZHANG Yu-Xun 한국소성가공학회 2010 기타자료 Vol.2010 No.6
Defects such as folding, intercrystalline cracking and flow lines outcrop are very likely to occur in the forging of aluminum alloy. Moreover, it is difficult to achieve the optimal set of process parameters just by trial and error within an industrial environment. In producing 7050 wrought aluminum alloy wheel, a rigid-plastic finite element method (FEM) analysis has been performed to optimize die forging process. Processing parameters were analyzed, focusing on the effects of punch speed, friction factor and temperature. Meanwhile, mechanism as well as the evolution with respect to the defects of the wrought wheel was studied in details. From an analysis of the results, isothermal die forging was proposed for producing 7050 aluminum alloy wheel with good mechanical properties. Finally, verification experiment was carried out on hydropress.