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RISS 인기검색어
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- 저자 : Xue Tian (검색결과 1 건)
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The Ethanol extracts of Saussurea lappa induced apoptosis and autophagy in prostate cancer cells
Xue Tian Gachon University 2017 국내석사
Saussurea lappa (SL) have been used as traditional herbal medicine for various disease, such as asthma, inflammatory disease, and ulcers. Extracts of Saussurea lappa have been shown in vitro to cause apoptosis in several types of cancer cells. To demonstrate the mechanisms by which the ethanol extract of Saussurea lappa (SLE) exerts a protective effect against prostate cancer, I evaluated the induction of apoptosis and autophagy by SLE in prostate cancer cells (LNCaP) cells. And we investigated whether SLE-induced autophagy exert pro-survival or pro-apoptotic effect in prostate cancer cell, LNCaP cells. Following optimization of the extract, viability of treated LNCaP cells was determined through the MTT assay using various concentrations of SLE (6.3-100 μg/mL). Cell apoptosis was evaluated using the Tali assay. The expression of apoptosis-related mRNA and proteins was analyzed by quantitative real-time RT-PCR and western blotting. Viability of LNCaP cells was found to be significantly reduced following SLE treatment for 24 h. I also found that SLE treatment increased the expression of Bax, tBid, cleaved caspase 3/8/9, cleaved PARP and FADD proteins while suppressed the expression of Bid and Bcl-2 in LNCaP cells, indicating the induction of apoptosis. PI3K/AKT/mTOR pathway-related proteins and autophagy-related proteins were evaluated by western blot assay. SLE was found to suppress phosphorylation and activation of AKT, AR, PSA, and PTEN, thus downregulating proteins related to cell proliferation in LNCaP cells. SLE exposure also resulted in stimulation of autophagy, as determined from the increase in levels of LC3I, LC3II and Beclin-1 proteins, and suppression of phosphorylation of mTOR. Finally, the inhibition of autophagy by exposure to 3-methyladenine lead to an increasing the apoptosis in LNCaP cell by SLE treatment was verified. The concurrent treatment of LNCaP cells with 3-methyladenine and SLE decreased the expression of anti-apoptotic protein Bcl-2 and increased the expression of pro-apoptotic protein Bax to a greater extent than did SLE treatment alone. These results suggest that SLE has anti-cancer effect by induction of cellular apoptosis and autophagy. And the apoptotic cell death could be improved by blocking of autophagy in SLE-treated LNCaP cells. However, this effect can be ameliorated by Saussurea lappa is a plant with strong potential as an effective anti-cancer drug for treatment of prostate cancer.
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