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Lu Liu,Hu Xu,Bing Shen,Xinhua Zhong 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2016 NANO Vol.11 No.7
Pentaerythritol tetrakis 3-mercaptopropionate (PTMP) grafted poly(acryl acid) (PAA) ionic hydrophilic oligomer PAA-PTMP (PP) and dihydrolipoic acid (DHLA) grafted methoxypoly (ethylene glycol) (mPEG) nonionic hydrophilic oligomer mPEG-DHLA (PD) have been designed, synthesized and used as co-capping ligands in water-solubilization of hydrophobic quantum dots (QDs) via ligand exchange. The obtained oligomers with multi-thiol groups could bind strongly to the surface atoms of QDs. Meanwhile, the carboxyl groups (from PP) and mPEG segment (from PD) can render QDs water-soluble, and the free carboxylic groups can possibly be used for the further bioconjugation. The resulting water-soluble QDs have been characterized by ultraviolet-visible (UV-Vis), fluorescence, Fourier transform infrared (FTIR) spectroscopy as well as transmission electron microscopy (TEM) and dynamic light scattering (DLS) techniques. The water-soluble QDs have relatively small hydrodynamic size (10-12 nm), and importantly, retain high fluorescence quantum yields (up to 45%) compared with that of the originally hydrophobic QDs (49%). In addition, they have tunable surface charges and show excellent colloidal stability over a relatively broad pH range (2-13), in high salt concentration, and even after thermal treatment at 100℃. These results indicate that the water-soluble QDs coated by PP and PD oligomers have potential applications in cellular imaging and biosensor.
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Limonoids from the barks of Chukrasia tabularis and their anti‑inflammatory activity
Shen Jinhuang,Zhang Yifan,Yang Nana,Ma Xinhua,Zhong Tianhua,Zhang Yonghong 한국응용생명화학회 2022 Applied Biological Chemistry (Appl Biol Chem) Vol.65 No.1
Two novel phragmalin type limonoids, Tabularisin Q (1) and Chuktabularin Y (18), together with 22 known limonoids have been separated from bark of Chukrasia tabularis. The structures of 1–24 were identified with spectroscopic method (NMR, IR and mass spectrometry) and comparison with literature. The anti-inflammatory activity of all limonoids was assayed in RAW264.7 cells in vitro by evaluating the production of nitric oxide induced by lipopolysaccharide. Limonoids 8, 9, 1, and 18 showed significant anti-inflammatory activity with the inhibitory rates of 4.32, 11.28, 13.13, and 10.40 μm, respectively, and likely to be useful for the development as therapeutic agents for inflammatory diseases.
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