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Tingzhang Hu,Zongli Hu,Hua Zeng,Xiaoxiao Qv,Guoping Chen 한국식물생명공학회 2015 Plant biotechnology reports Vol.9 No.1
TomloxD is a lipoxygenase from Lycopersiconesculentum Mill, which has lipoxygenase activity. Theexpression of TomloxD can be stimulated by wounding,pathogen infection, jasmonate, and systemin. To investigatethe function of TomloxD, transgenic tomato plantssilencing tomloxD gene was produced. The suppression ofTomloxD expression led to a marked reduction in the levelsof lipoxygenase activity and endogenous jasmonic acidcontent, which suggested TomloxD can catalyze a-linolenicacids to produce (13S)-hydroperoxyoctadecatrienoicacid (13-HPOT), and the 13-HPOT is metabolized furtherto synthesize jasmonic acid. Real-time RT-PCR revealedthat the expression of defense genes LeHSP90, LePR1 andLePR6 was also less in the transformants than in the wildtypetomato plant. Resistance assay showed that the suppressionof TomloxD in transgenic tomato plants reducedthermotolerance of tomato and increased its susceptibilityto Cladosporium fulvum. Collectively, the data presentedhere suggest that the TomloxD plays a role as a componentof the octadecanoid defense-signaling pathway andinvolved in the generation of endogenous jasmonic acid,and in turn regulates the expression of plant defense genesand resistance to high temperature and pathogen attack.