http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Hao Zhang,Xiaoning Liang,Xiao Yu Zhao,Zhong Peng Cao 글로벌지식마케팅경영학회 2014 Global Marketing Conference Vol.2014 No.3
Customers are regarded as the key intangible assets of a company. It is necessary to have the capability to anticipate customer value. The study discusses the relationship among customer value anticipation, product innovativeness, and customer lifetime value from customer perspective. 178 MBA students were surveyed by questionnaire in this study. The results show that customer perceived customer value anticipation can significantly influence product innovativeness. Product innovativeness not only positively affects customer lifetime value, but also plays a partial mediating role between customer value anticipation and customer lifetime value. Both functional and emotional advertising can play a moderating role on the relationship between product innovativeness and customer lifetime value. At the end, managerial implications are discussed.
Hao Zhang,Xiaoning Liang,Xiao Yu Zhao,Zhong Peng Cao 글로벌지식마케팅경영학회 2014 Global Marketing Conference Vol.2014 No.7
Customers are regarded as the key intangible assets of a company. It is necessary to have the capability to anticipate customer value. The study discusses the relationship among customer value anticipation, product innovativeness, and customer lifetime value from customer perspective. 178 MBA students were surveyed by questionnaire in this study. The results show that customer perceived customer value anticipation can significantly influence product innovativeness. Product innovativeness not only positively affects customer lifetime value, but also plays a partial mediating role between customer value anticipation and customer lifetime value. Both functional and emotional advertising can play a moderating role on the relationship between product innovativeness and customer lifetime value. At the end, managerial implications are discussed.
Biochemical Properties and Physiological Roles of NADP-Dependent Malic Enzyme in Escherichia coli
Baojuan Wang,Peng Wang,Enxia Zheng,Xiangxian Chen,Hanjun Zhao,Ping Song,Ruirui Su,Xiaoning Li,Guoping Zhu 한국미생물학회 2011 The journal of microbiology Vol.49 No.5
Malic enzymes catalyze the reversible oxidative decarboxylation of L-malate using NAD(P)^+ as a cofactor. NADP-dependent malic enzyme (MaeB) from Escherichia coli MG1655 was expressed and purified as a fusion protein. The molecular weight of MaeB was about 83 kDa, as determined by SDS-PAGE. The recombinant MaeB showed a maximum activity at pH 7.8 and 46°C. MaeB activity was dependent on the presence of Mn^(2+) but was strongly inhibited by Zn^(2+). In order to understand the physiological roles, recombinant E. coli strains (icdNADP/ΔmaeB and icdNAD/ΔmaeB) containing NADP-dependent isocitrate dehydrogenase (IDH), or engineered NAD-dependent IDH with the deletion of the maeB gene, were constructed using homologous recombination. During growth on acetate, icd^(NAD)/ΔmaeB grew poorly, having a growth rate only 60% that of the wild-type strain (icd^(NADP)). Furthermore, icd^(NAD)P/ΔmaeB exhibited a 2-fold greater adaptability to acetate than icdNAD/ΔmaeB, which may be explained by more NADPH production for biosynthesis in icd^(NAD)P/ΔmaeB due to its NADP-dependent IDH. These results indicated that MaeB was important for NADPH production for bacterial growth on acetate. We also observed that MaeB activity was significantly enhanced (7.83-fold) in icd^(NAD), which was about 3-fold higher than that in icd^(NADP), when switching from glucose to acetate. The marked increase of MaeB activity was probably induced by the shortage of NADPH in icd^(NAD). Evidently, MaeB contributed to the NADPH generation needed for bacterial growth on two carbon compounds.