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Xiao Xigang,Xi Xun,Xiao Sujian,Ni Jun 대한독성 유전단백체 학회 2022 Molecular & cellular toxicology Vol.18 No.4
Background Numerous circular RNAs (circRNAs) are functionally investigated in human cancers. However, there are still a large number of circRNAs with unclear functions. Objective The objective of this study was to determine the role of circ_0101622 in thyroid cancer. The expression of circ_0101622, microRNA-1179 (miR-1179), and Ras-associated binding protein 23 (RAB23) was measured using quantitative real-time PCR method. The protein levels of E-cadherin, N-cadherin, and RAB23 were detected by western blot. For functional analyses, cell proliferation, apoptosis, migration, and invasion were assessed using cell counting kit-8 (CCK-8) assay, flow cytometry assay, wound-healing assay, and Transwell assay, respectively. Glycolysis was assessed by glucose consumption and lactate production. The binding between miR-1179 and circ_0101622 or RAB23 was determined by dualluciferase reporter assay and RIP assay. Animal study was performed to verify the role of circ_0101622. Circ_0101622 showed high expression in thyroid cancer tissues and cells. Results Circ_0101622 knockdown inhibited cell proliferation, migration, invasion, and glycolysis metabolism in vitro and blocked tumor growth in vivo. Mechanism analysis found that circ_0101622 functioned as miR-1179 sponge to release RAB23. Circ_0101622 knockdown inhibited cancer development by enriching miR-1179, and miR-1179 restoration inhibited cancer development by sequestering RAB23. Conclusion Circ_0101622 might act as an oncogenic driver to promote the malignant progression of thyroid cancer by mediating the miR-1179/RAB23 pathway.
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