Transcriptome profiling of rubber tree (Hevea brasiliensis) discovers candidate regulators of the cold stress respo

Xiao‑Xiao Gong,Bing‑Yu Yan,Jin Hu,Cui‑Ping Yang,Yi‑Jian Li,Jin‑Ping Liu,Wen‑Bin Liao 한국유전학회 2018 Genes & Genomics Vol.40 No.11

Tropical plant rubber tree (Hevea brasiliensis) is the sole source of commercial natural rubber and low-temperature stress is the most important limiting factor for its cultivation. To characterize the gene expression profiles of H. brasiliensis under the cold stress and discover the key cold stress-induced genes. Three cDNA libraries, CT (control), LT2 (cold treatment at 4 °C for 2 h) and LT24 (cold treatment at 4 °C for 24 h) were constructed for RNA sequencing (RNA-Seq) and gene expression profiling. Quantitative real time PCR (qRT-PCR) was conducted to validate the RNA-Seq and gene differentially expression results. A total of 1457 and 2328 differentially expressed genes (DEGs) in LT2 and LT24 compared with CT were respectively detected. Most significantly enriched KEGG pathways included flavonoid biosynthesis, phenylpropanoid biosynthesis, plant hormone signal transduction, cutin, suberine and wax biosynthesis, Pentose and glucuronate interconversions, phenylalanine metabolism and starch and sucrose metabolism. A total of 239 transcription factors (TFs) were differentially expressed following 2 h or/and 24 h of cold treatment. Cold-response transcription factor families included ARR-B, B3, BES1, bHLH, C2H, CO-like, Dof, ERF, FAR1, G2-like, GRAS, GRF, HD-ZIP, HSF, LBD, MIKC-MADS, M-type MADS, MYB, MYB-related, NAC, RAV, SRS, TALE, TCP, Trihelix, WOX, WRKY, YABBY and ZF-HD. The genome-wide transcriptional response of rubber tree to the cold treatments were determined and a large number of DEGs were characterized including 239 transcription factors, providing important clues for further elucidation of the mechanisms of cold stress responses in rubber tree.

Survival and Performance of Two Cellulose-Degrading Microbial Systems Inoculated into Wheat Straw-Amended Soil

( Pei Pei Li ),( Dong Dong Zhang ),( Xiao Juan Wang ),( Xiao Fen Wang ),( Zong Jun Cui1 ) 한국미생물 · 생명공학회 2012 Journal of microbiology and biotechnology Vol.22 No.1

A cellulose-degrading composite microbial system containing a mixture of microbes was previously shown to demonstrate a high straw-degrading capacity. To estimate its potential utilization as an inoculant to accelerate straw biodegradation after returning straw to the field, two cellulose-degrading composite microbial systems named ADS3 and WSD5 were inoculated into wheat straw-amended soil in the laboratory. The microbial survival of the inoculant was confirmed by a denaturing gradient gel electrophoresis (DGGE) analysis, whereas the enhancement of straw degradation in soil was assessed by measuring the mineralization of the soil organic matter and the soil cellulase activity. The results indicated that most of the DGGE bands from ADS3 were detected after inoculation into straw-amended autoclaved soil, yet only certain bands from ADS3 and WSD5 were detected after inoculation into straw-amended non-autoclaved soil during five weeks of incubation; some bands were detected during the first two weeks after inoculation, and then disappeared in later stages. Organic matter mineralization was significantly higher in the soil inoculants ADS3 and WSD5 than in the uninoculated controls during the first week, yet the enhanced degradation did not persist during the subsequent incubation. Similar to the increase in soil organic matter, the cellulase activity also increased during the first week in the ADS3 and WSD5 treatments, yet decreased during the remainder of the incubation period. Thus, it was concluded that, although the survival and performance of the two inoculants did not persist in the soil, a significant enhancement of degradation was present during the early stage of incubation.

Interval-valued Analytic Network Process and its Application in Risk Assessment of Dynamic Alliance

Xiao-Guang Zhou,Yan-Hui Zhou,Xiao-Xiao Cui 보안공학연구지원센터 2015 International Journal of u- and e- Service, Scienc Vol.8 No.9

Analytic Network Process (ANP) has been widely used in solving many complicated decision making problems. An approach to acquire the comprehensive weights of interval-valued Analytic Network Process (IV-ANP) is proposed. Firstly, the independent local interval-valued weights are obtained by solving the goal programming for interval-valued pairwise comparison matrices. Based on the interaction and feedback relationships among criteria and/or indices, an unweighted interval-valued supermatrix is formed. To avoid loss of information, an extreme value method is developed for acquiring the global weights of IV-ANP. Secondly, the alternatives are ranked on the basis of interval-valued preference order. Finally, with the interaction and feedback relationships between criteria and/or indices being considered, an index system for assessing the risk of dynamic alliance is presented. With the uncertain and inaccurate information during the evaluation process being considered, the proposed IV-ANP method is applied for risk assessment of dynamic alliance, and sensitivity analyses for some key risk factors are performed to show their impacts on the risk levels of alliance enterprises.

Prognostic Value of PLCE1 Expression in Upper Gastrointestinal Cancer: a Systematic Review and Meta-analysis

Cui, Xiao-Bin,Peng, Hao,Li, Su,Li, Ting-Ting,Liu, Chun-Xia,Zhang, Shu-Mao,Jin, Ting-Ting,Hu, Jian-Ming,Jiang, Jin-Fang,Liang, Wei-Hua,Li, Na,Li, Li,Chen, Yun-Zhao,Li, Feng Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.22

Background: A number of studies have identified a shared susceptibility locus in phospholipase C epsilon 1 (PLCE1) for esophageal squamous cell carcinoma (ESCC) and gastric cardia adenocarcinomas (GCA). However, the results of PLCE1 expression in esophageal and gastric cancer remain inconsistent and controversial. Moreover, the effects on clinicopathological features remain undetermined. This study aimed to provide a precise quantification of the association between PLCE1 expression and the risk of ESCC and GCA through meta-analysis. Materials and Methods: Eligible studies were identified from PubMed, Wanfang Data, ISI Web of Science, and the Chinese National Knowledge Infrastructure databases. Using RevMan5.2 software, pooled odds ratios (ORs) with 95% confidence intervals (CIs) were employed to assess the association of PLCE1 expression with clinicopathological features relative to ESCC or GCA. Results: Seven articles were identified, including 761 esophageal and gastric cancer cases and 457 controls. Overall, we determined that PLCE1 expression was associated with tumor progression in both esophageal cancers (pooled OR=5.93; 95%CI=3.86 to 9.11) and gastric cancers (pooled OR=9.73; 95%CI=6.46 to 14.7). Moreover, invasion depth (pooled OR=3.62; 95%CI=2.30 to 5.70) and lymph node metastasis (pooled OR=4.21; 95%CI=2.69 to 6.59) were linked with PLCE1 expression in gastric cancer. However, no significant associations were determined between PLCE1 overexpression and the histologic grade, invasion depth, and lymph node metastasis in esophageal cancer. Conclusions: Our metaanalysis results indicated that upregulated PLCE1 is significantly associated with an increased risk of tumor progression in ESCC and GCA. Therefore, PLCE1 expression can be appropriately regarded as a promising biomarker for ESCC and GCA patients.

Radiosensitivity Enhancement by Arsenic Trioxide in Conjunction with Hyperthermia in the EC-1 Esophageal Carcinoma Cell Line

Cui, Yan-Hui,Liang, Hai-Jun,Zhang, Qing-Qin,Li, Si-Qing,Li, Xiao-Rui,Huo, Xiao-Qing,Yang, Qing-Hui,Li, Wei-Wei,Gu, Jian-Fa,Hua, Qin-Liang,Lu, Ping,Miao, Zhan-Hui Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.4

Objective: To explore the effect on radiosensitivity of arsenic trioxide ($As_20_3$) in conjunction with hyperthermia on the esophageal carcinoma EC-1 cell line. Method: Inhibition of EC-1 cell proliferation at different concentrations of $As_20_3$ was assessed using the methyl thiazolyl blue colorimetric method (MTT method), with calculation of $IC_{50}$ value and choice of 20% of the $IC_{50}$ as the experimental drug concentration. Blank control, $As_20_3$, hyperthermia, radiotherapy group, $As_20_3$ + hyperthermia, $As_20_3$ + radiotherapy, hyperthermia + radiotherapy and $As_20_3$ + hyperthermia + radiotherapy groups were established, and the cell survival fraction (SF) was calculated from flat panel colony forming analysis, and fitted by the 'multitarget click mathematical model'. Flow cytometry (FCM) was used to detect changes in cell apoptosis and the cell cycle. Results: $As_20_3$ exerted inhibitory effects on proliferation of esophageal carcinoma EC-1 cells, with an $IC_{50}$ of 18.7 ${\mu}mol/L$. After joint therapy of $As_20_3$ + hyperthermia + radiotherapy, the results of FCM showed that cells could be arrested in the $G_2$/M phase, and as the ratio of cells in $G_0/G_1$ and S phases decreased, cell death became more pronounced. Conclusion: $As_20_3$ and hyperthermia exert radiosensitivity effects on esophageal carcinoma EC-1 cells, with synergy in combination. Mechanistically, $As_20_3$ and hyperthermia mainly influence the cell cycle distribution of EC-1 esophageal carcinoma cells, decreasing the repair of sublethal damage and inducing apoptosis, thereby enhancing the killing effects of radioactive rays.

Syntheses, Structures, and Luminescence Properties of Two Copper(II) Complexes Constructed by Rigid Bis(triazole) and Nitrogen-containing Carboxylic Acid Ligands

Xiao-Xiao,Zuo-Xi Li,Bao-yi Yu,Guang-hua Cui 대한화학회 2015 Bulletin of the Korean Chemical Society Vol.36 No.7

Two new copper(II) complexes with the formulas [Cu(btb)(pydc)(H2O)]n (1) and [Cu(btb)0.5(nph)(H2O)]n (2) have been synthesized under hydrothermal conditions by employing a rigid bis(triazole) ligand (btb = 4,4′-bis(1,2,4-triazolyl-1-yl)-biphenyl) and two carboxylic acids mixed ligands (H2pydc = pyridine-2,5-dicarboxylic acid and H2nph = 3-nitrophthalic acid). The copper atoms present different environments, with a tetragonal pyramidal geometry in 1 and octahedral configuration in 2. Complex 1 displays a dinuclear cluster, which is further packed into a 2D supramolecular layer by classical OH · · · O hydrogen bonds. Complex 2 possesses a trinodal 3,4,4-connected 3D framework with a rare sqc69 topology. In addition, thermal stability and luminescence property were investigated.

Natural Organic Matter Removal and Fouling Control in Low-Pressure Membrane Filtration for Water Treatment

Xiao Jun Cui,Kwang Ho Choo 대한환경공학회 2014 Environmental Engineering Research Vol.19 No.1

Natural organic matter (NOM) is a primary component of fouling in low-pressure membrane filtration, either solely, or in concert with colloidal particles. Various preventive measures to interfere with NOM fouling have been developed and extensively tested, such as coagulation, oxidation, ion exchange, carbon adsorption, and mineral oxide adsorption. Therefore, this article aims to conduct a literaturere view covering the topics of low-pressure membrane processes, NOM characteristics and fouling behaviors, and diverse fouling control strategies. In-depth explanations and discussion are made regarding why some treatment options are able to remove NOM from source water, but do not reduce fouling. This review provides insight for hybridized membrane processes with respect to NOM removal and fouling mitigation in water treatment.

Upregulation of MicroRNA 181c Expression in Gastric Cancer Tissues and Plasma

Cui, Mei-Hua,Hou, Xiao-Lin,Lei, Xiao-Yan,Mu, Fang-Hong,Yang, Gui-Bin,Yue, Lin,Fu, Yi,Yi, Guo-Xing Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.5

Objective: To test the microRNA-181c (miR-181c) expression in tissues and plasma of gastric cancer (GC) cases, analyze any correlations, and explore the possibility of miR-181c as a potential molecular marker for GC diagnosis. Materials and Methods: Relative miR-181c expression levels in cancers and plasma from 30 GC patients was tested using reverse transcription-real-time fluorescent quantitation PCR and compared to that in samples from 30 gastric ulcer and 30 chronic gastritis patients. Results: The miR-181c expression level in the GC tissues was significantly higher than that in the gastric ulcer and chronic gastritis tissues (P = 0.000), as was the miR-181c expression level in the GC plasma (P = 0.000). We determined that miR-181c expression in GC plasma was positively correlated to its expression in the GC tissues (P = 0.000). Conclusions: The expression of miR-181c is upregulated in GC tissues and plasma, and the miR-181c expression level in GC plasma is positively correlated to that in the corresponding cancer tissues. Plasma miR-181c is possibly a new serological marker for GC diagnosis.

Instability of a Viscoelastic Jet Containing Micro-nano Bubbles

( Xiao Cui ),( Luo Xie ),( Lijun Yang ) 한국액체미립화학회 2017 한국액체미립화학회 학술강연회 논문집 Vol.2017 No.-

An effervescent atomizer is a valid applications to obtain small droplets. It can potentially solve the problem of clogging, erosion, high injection pressure, huge gas consumption. To further improve the atomization performance of effervescent atomizer, it is critical to explore the theory behind the onset of instability of viscoelastic jet containing micro-nano bubbles. This paper study the linear instability of that jet surrounded by inviscid gas. Compressibility and viscoelasticity caused by micro-nano gas bubbles are taken into account, since that is consistent with the actual rheological property of jet (Llewellin E W, Mader H M, Sdr W. “The rheology of a bubbly liquid,” Proceedings Mathematical Physical & Engineering Sciences,(2020) 2002). Adopting the method of linear instability to analyze the temporal development of disturbance for jet and obtaining the relationship of wave number and growth rate by utilizing spectral method. It is found that a viscoelastic jet is more unstable than a Newtonian one. Moreover, results show that there exist two instable areas by considering the variation of the radii of gas bubbles and gas volume fraction of bubbles make a great influence of the instability of viscoelastic. The viscoelasticity and compressibility of the jet also make it unstable. To make out the deep mechanism of above results, an analysis of the energy budget of destabilization process is performed, in which a formulation from momentum conservation equation. The formulation is observed to provide a quantitative analysis of the contribution of each physical factor (e.g., release of surface energy, viscous dissipation and compressibility work).The energy analysis reveals that the viscous force, aerodynamic, surface tense common rules the instability of the bubble jet. The two unstable areas because of the competition of these forces.

Research on Load Balance Method in SDN

Cui Chen-xiao,Xu Ya-bin 보안공학연구지원센터 2016 International Journal of Grid and Distributed Comp Vol.9 No.1

In order to improve the performance of data transmission in SDN, this paper proposes a load balance solution scheme by taking advantage of the global network view of SDN. We collect 4 load features from each transmission path. These features are bandwidth utilization ratio, packet loss rate, transmission latency and transmission hops. By using this 4 load features, BP Artificial Neural Network model is trained to predict the integrated load for different path and to choose one with least load as the data-flow transmission path. The contrast experiment results show that load balancing strategy proposed in this paper can select more rational transmission path for data-flow and achieve 19.3% network latency decrease at most.