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Kim, Jeong Myeong,Jung, Ji Young,Chae, Ho Byoung,Park, Woojun,Jeon, Che Ok Society for General Microbiology 2010 International journal of systematic and evolutiona Vol.60 No.12
<P>A moderately halophilic Gram-staining-negative bacterium, designated strain Y26(T), was isolated from a tidal flat of Taean coast in South Korea. Cells were strictly aerobic, motile cocci with a single flagellum and showed catalase- and oxidase-positive reactions. Growth of strain Y26(T) was observed at 15-35 C (optimum 25-30C), pH 6.0-8.0 (optimum pH6.5-7.5) and with 1.5-6.0?% (w/v) NaCl (optimum 2.0-3.0?%). The predominant fatty acids were C(18?:?1)ω7c (66.2?%), C(16?:?0) (12.4?%) and C(10?:?0) 3-OH (5.0?%) and the G+C content of the genomic DNA was 61.0 mol%. Strain Y26(T) contained ubiquinone-10 (Q-10) as the major respiratory quinone. Comparative 16S rRNA gene sequence analysis showed that strain Y26(T) formed a distinct phyletic lineage from other genera within the Roseobacter clade of the class Alphaproteobacteria and was most closely related to members of the genera Maribius, Maritimibacter and Palleronia with 93.8-94.6?% sequence similarity. On the basis of chemotaxonomic data and molecular properties, strain Y26(T) represents a novel genus, Hwanghaeicola, within the family Rhodobacteraceae, for which the name Hwanghaeicola aestuarii gen. nov., sp. nov. is proposed. The type strain is Y26(T) (=KACC 13705(T) =DSM 22009(T)).</P>
Kim, Jeong Myeong,Lee, Hyo Jung,Kim, Sun Young,Song, Jae Jun,Park, Woojun,Jeon, Che Ok American Society for Microbiology 2010 Applied and environmental microbiology Vol.76 No.12
<B>ABSTRACT</B><P>To investigate the fine-scale diversity of the polyphosphate-accumulating organisms (PAO) “<I>Candidatus</I> Accumulibacter phosphatis” (henceforth referred to as “<I>Ca.</I> Accumulibacter”), two laboratory-scale sequencing batch reactors (SBRs) for enhanced biological phosphorus removal (EBPR) were operated with sodium acetate as the sole carbon source. During SBR operations, activated sludge always contained morphologically different “<I>Ca</I>. Accumulibacter” strains showing typical EBPR performances, as confirmed by the combined technique of fluorescence <I>in situ</I> hybridization (FISH) and microautoradiography (MAR). Fragments of “<I>Ca.</I> Accumulibacter” 16S rRNA genes were retrieved from the sludge. Phylogenetic analyses together with sequences from the GenBank database showed that “<I>Ca.</I> Accumulibacter” 16S rRNA genes of the EBPR sludge were clearly differentiated into four “<I>Ca.</I> Accumulibacter” clades, Acc-SG1, Acc-SG2, Acc-SG3, and Acc-SG4. The specific FISH probes Acc444, Acc184, Acc72, and Acc119 targeting these clades and some helpers and competitors were designed by using the ARB program. Microbial characterization by FISH analysis using specific FISH probes also clearly indicated the presence of different “<I>Ca.</I> Accumulibacter” cell morphotypes. Especially, members of Acc-SG3, targeted by probe Acc72, were coccobacillus-shaped cells with a size of approximately 2 to 3 μm, while members of Acc-SG1, Acc-SG2, and Acc-SG4, targeted by Acc444, Acc184, and Acc119, respectively, were coccus-shaped cells approximately 1 μm in size. Subsequently, cells targeted by each FISH probe were sorted by use of a flow cytometer, and their polyphosphate kinase 1 (<I>ppk1</I>) gene homologs were amplified by using a <I>ppk1</I>-specific PCR primer set for “<I>Ca.</I> Accumulibacter.” The phylogenetic tree based on sequences of the <I>ppk1</I> gene homologs was basically congruent with that of the 16S rRNA genes, but members of Acc-SG3 with a distinct morphology comprised two different <I>ppk1</I> genes. These results suggest that “<I>Ca.</I> Accumulibacter” strains may be diverse physiologically and ecologically and represent distinct populations with genetically determined adaptations in EBPR systems.</P>
Binhee Kwon,Jongyeap Park,Woojun Jeong,Guembi Jeong,Hyeong Seon Ryu,Peerasak Paoprasert,Sung Young Park,Insik In 한국탄소학회 2018 Carbon Letters Vol.27 No.-
To formulate folate receptor (FR)-specific graphene-based electrochemical electrodes, a folic acid (FA) derivative attached with two pyrene molecules on the glutamate tail of FA was synthesized. The resulting pyrene-functionalized FA (FA-Py) presented the spontaneous noncovalent binding on chemically reduced graphene oxides (rGO) through an π-π interaction. Ultrathin morphology, high water-resistance, and preservation of intact FR-specific pteroates from the rGO/FA-Py assembly allow this assembly to be exploited as robust and FR-specific electrochemical electrode materials. The limits of detecting rGO/FA-Py modified electrodes were found to be as low as 3.07 nM in FR concentrations in cyclic voltammetry analysis
Self-Assembling Peptide Nanofibers Coupled with Neuropeptide Substance P for Bone Tissue Engineering
Kim, Su Hee,Hur, Woojune,Kim, Ji Eun,Min, Hye Jeong,Kim, Sukwha,Min, Hye Sook,Kim, Byeung Kyu,Kim, Soo Hyun,Choi, Tae Hyun,Jung, Youngmee Mary Ann Liebert 2015 Tissue engineering. Part A Vol.21 No.7
<P>The number of patients requiring flat bone transplantation continues to increase worldwide. Cell transplantation has been successfully applied clinically; however, it causes another defect site and the time requirements to harvest cells and expand them are considerable. In this study, KLD12/KLD12-SP (KLD12+KLD12-substance P [SP]) was designed to mimic endogenous tissue-healing processes. The structures of KLD12, KLD12-SP, and KLD12/KLD12-SP were observed by transmission electron microscopy and circular dichroism spectra. KLD12/KLD12-SP nanofibers (5-10?nm) were created under physiological conditions by formation of a β-sheet structure. The ability of mesenchymal stem cells (MSCs) to recruit KLD12/KLD12-SP was observed by using an in vivo fluorescence imaging system. Labeled human bone marrow stromal cells supplied via an intravenous injection were recruited to the scaffold containing KLD12/KLD12-SP. Polylactic acid/beta-tricalcium phosphate (PLA/β-TCP) scaffolds filled with KLD12/KLD12-SP were applied to repair calvarial defects. The composite constructs (groups: defect, PLA/β-TCP, PLA/β-TCP/KLD12, and PLA/β-TCP/KLD12/KLD12-SP) were implanted into rat defect sites. Bone tissue regeneration was evaluated by observing gross morphology by hematoxylin and eosin and Masson's trichrome staining at 12 and 24 weeks after surgery. Gross morphology showed that the defect site was filled with new tissue that was integrated with host tissue in the KLD12/KLD12-SP group. In addition, from the staining data, cells were recruited to the defect site and lacunae structures formed in the KLD12/KLD12-SP group. From these results, the PLA/β-TCP+KLD12/KLD12-SP composite construct was considered for enhancement of bone tissue regeneration without cell transplantation.</P>