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Woo‑Ri Shin,Mun‑Jong Lee,SEKHONSIMRANJEET SINGH,Ji Hun Kim,Sun Chang Kim,Byung‑Kwan Cho,Ji‑Young Ahn,Yang‑Hoon Kim 대한독성 유전단백체 학회 2020 Molecular & cellular toxicology Vol.16 No.3
Background Development of genetically modified crops has rapidly increased in last few years. The most widely grown GM crops express genes that confer herbicide tolerance and insect resistance. Detection system of GM crops is important for safety evaluation before its consumption. Objective The purpose of this research is to detect GM crops, especially PAT, in food-samples. Results The bar gene (PAT protein, herbicide resistant) was cloned in pGEX-4T-1 and expressed by E. coli. The high-affinity PAT-specific single-stranded DNA (ssDNA) aptamers were obtained from a random DNA library. MOE docking study was performed to identify the potential binding region of the selected aptamers on PAT. Aptamer-linked immobilized sorbent assay (ALISA) method was used to detect PAT. Conclusion We screened aptamer against PAT for developing an efficient detection method. The selected PAT specific aptamers, HRPA-05 and HRPA-07, showed the distinct target binding behaviors, and detected PAT protein by aptamer-linked immobilized sorbent assay method with high efficiency and selectivity.
신에스더,박희서,이시예,송우리,김명철,김덕환,송근호 충남대학교 수의과대학 동물의과학연구소 2004 動物醫科學硏究誌 Vol.12 No.-
A 3-month-old, 3.85kg female Jin-do dog with chief complaint of bloody diarrhea and depression was referred to the Veterinary Teaching Hospital, Chungnam National University. In fecal examination, trophozoites of giardia were observed and It was diagnosed as canine giardiasis. For treatment, metronidazole was administered orally for 5 days at a dose of 22mg/kg q12h. Diarrhea was not observed on 3 days after medication and most of clinical signs were disappeared on 7 days after medication with metronidazole. General conditions and activitivities became good on 13 days after medication. In addition, cysts or trophozoites of giardia were not detected any more in fecal examination using floating method with zinc sulphate solution. The present patient was a case of canine giardiasis that showed favorable response to metronidazole.
송우리,이시예,신에스더,박희서,정성목,송근호,김명철 충남대학교 수의과대학 동물의과학연구소 2004 動物醫科學硏究誌 Vol.12 No.-
A 4 years old, mixed male dog with a history of traffic accident was presented to the Veterinary Medical Teaching Hospital of Chungnam National University with tachypnea and respiratory distress. Physical examination showed that the dog was auscultated crackles sounds, dysynchronous abdominal breathing pattern, hemoptysis, hematuria, urodynia and right and left scleral congestion. Blood and blood chemical examination revealed that WBC, AST and ALT was highly elevated. Parenchymal pulmonary fluid and closed pneumothorax were observed by thoracic radiography. This case was diagnosed as pulmonary contusion. Intravenous fluid and oxygen was administered and also dexamethasone(1mg/kg, Ⅳ), furosemide(2mg/kg, Ⅳ), (ampicillin, 20mg/kg, Ⅳ) and meloxicam(0.2mg/kg, PO) were intravenously injected for 5 days. Abnormal clinical signs were disappeared after treatment.
3D structural analysis of aptamer and diagnostic platforms for detecting hepatocellular carcinoma
Shin Woo-Ri,박대영,Um Hyun-Ju,Ahn Gna,김상용,안지영,김양훈 대한독성 유전단백체 학회 2023 Molecular & cellular toxicology Vol.19 No.3
Background Alpha-fetoprotein (AFP) has been used as a biomarker for early diagnosis of hepatocellular carcinoma (HCC) for a long time. Aptamer is a type of material that is receiving attention as a new alternative in the diagnosis and treatment of diseases. Objective We applied aptamer to AFP detection and utilized in silico analysis techniques. Here, we describe the selection of aptamers that can bind to AFP as well as in vitro and in silico validation of the binding site. We also developed an aptamerbased detection platform called hybrid-aptablotting sandwich assay (Hybrid-ABSA) and a point-of-care AFP detection kit. Results To construct the AFP detection platform, we screened a total of 18 candidate AFP-binding aptamers and identifi ed a pair of aptamers without non-overlapping-binding sites using dot blotting. We validated results of dot blotting using in silico 3D binding structure docking simulation analysis. The aptamer-based Hybris-ABSA for AFP detection using this pair of aptamers demonstrated superior detection performance to antibody-based ELISA detection methods. Furthermore, we confi rmed that AFP could be detected using the developed point-of-care lateral fl ow In Vitro Diagnostic (IVD) kit. Conclusion Results of this study demonstrate that aptamer-based disease diagnostic technology has potential of replace conventional diagnostic methods. This study also confi rms that in silico techniques have suffi cient utility in biological assays.
Aptamer-Based Paper Strip Sensor for Detecting <i>Vibrio fischeri</i>
Shin, Woo-Ri,Sekhon, Simranjeet Singh,Rhee, Sung-Keun,Ko, Jung Ho,Ahn, Ji-Young,Min, Jiho,Kim, Yang-Hoon American Chemical Society 2018 ACS Combinatorial Science Vol.20 No.5
<P>Aptamer-based paper strip sensor for detecting <I>Vibrio fischeri</I> was developed. Our method was based on the aptamer sandwich assay between whole live cells, <I>V. fischeri</I> and DNA aptamer probes. Following 9 rounds of Cell-SELEX and one of the negative-SELEX, <I>V. fischeri</I> Cell Aptamer (VFCA)-02 and -03 were isolated, with the former showing approximately 10-fold greater avidity (in the subnanomolar range) for the target cells when arrayed on a surface. The colorimetric response of a paper sensor based on VFCA-02 was linear in the range of 4 × 10<SUP>1</SUP> to 4 × 10<SUP>5</SUP> CFU/mL of target cell by using scanning reader. The linear regression correlation coefficient (<I>R</I><SUP>2</SUP>) was 0.9809. This system shows promise for use in aptamer-conjugated gold nanoparticle probes in paper strip format for in-field detection of marine bioindicating bacteria.</P> [FIG OMISSION]</BR>
Development of Dual-functional Aptamer Complex that Inhibit the Pancreatic Cancer Growth
Woo-Ri SHIN,Hee-Young CHO,Ji-Young AHN,Yang-Hoon KIM 한국생물공학회 2021 한국생물공학회 학술대회 Vol.2021 No.10
Pancreatic cancer is hard to diagnose early and are not easily treated once they spread to other organs. The aptamer that have been treated with pancreatic cancer, will be targeted ALPPL2 expressed on the surface of pancreatic cancer cell and downregulated the CTHRC1 function by blocking the Smad. We selected the aptamer against CTHRC1/ALPPL2 protein. Then, we analyzed the binding structure of the protein-aptamer complex, which is confirmed the RNA aptamer bind to the active site of the protein. Aptamers of each binding to CTHRC1/ALPPL2 were conjugated to produce an aptamer complex with two functions that specifically bind to surface and inhibit the growth of pancreatic cancer cell.
Woo Ri Shin(신우리) 위기관리 이론과 실천 2018 Crisisonomy Vol.14 No.9
본 연구는 재난관리 교육훈련 프로그램 특성과 교육훈련 효과성의 관계에서 재난관리 업무를 담당 하는 공무원의 역량이 미치는 영향을 살펴보고자 하였다. 이를 위하여 20개 지역의 572명의 지방자치단체 공무원에게 설문조사를 실시하고 구조방정식 모델(SEM)을 사용하여 분석을 수행하였다. 그 결과 재난관리 교육훈련 프로그램 특성은 직접적인 영향은 없으나 재난관리 공무원 역량에 직접적 인 영향을 미치며, 재난관리 공무원 역량은 재난관리 교육훈련 효과성에 직접적인 영향을 미치는 것으로 나타났다. 이 같은 결과는 공무원의 역량을 향상시켜야만 교육훈련을 통해 습득한 지식, 태도 등을 업무에 적용하는 교육훈련의 효과성이 향상될 수 있다는 것을 의미하는 것으로, 교육훈련의 최종목표인 효과성의 향상을 위해서는 단순히 교육시수를 늘리는 것이 아니라 공무원의 역량을 향상시킬 수 있는 방향으로 교육훈련이 운영되어야 함을 의미한다. This study attempts to examine the effect of the capacity of civil servants on the characteristics and effectiveness of education and training programs on disaster management. To increase the effective-ness of education and training programs, it is necessary to primarily develop strategies to improve their interpersonal abilities in terms of characteristics such as diversity, cooperation, and sustainability. By examining the mediating effects of the capacity of disaster management officials on the relationship between the characteristics of education and training programs and their effectiveness, this study identified which characteristics of education and training programs should be enhanced to improve the capacity of disaster management officials and improve the effectiveness of education and training programs.