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Selection of F2 Genotype Resistant to Soybean Cyst Nematode Using Molecular Markers
Won Gyeong Park,Myung Sik Kim,Mi Suk Ko,Jin Ho Kang,Jong Il Chung 한국육종학회 2003 한국육종학회지 Vol.35 No.3
Soybean cyst nematode (Heterodera glycines Ichinohe, SCN) is an important soybean pest and the use of resistant cul-tivars is an effective method to reduce or eliminate SCN damage. SCN resistance loci, rhg1 and Rhg4 are generally accepted as anecesity for
Park, Hyeon Soo,Hwang, Yong Hyeon,Kim, Mun Ki,Hong, Gyeong Eun,Lee, Ho Jeong,Nagappan, Arulkumar,Yumnam, Silvia,Kim, Eun Hee,Heo, Jeong Doo,Lee, Sang Joon,Won, Chung Kil,Kim, Gon Sup Japan Society for Bioscience, Biotechnology, and A 2015 Bioscience, Biotechnology, and Biochemistry Vol.79 No.1
<P>Grifola frondosa (GF), distributed widely in far east Asia including Korea, is popularly used as traditional medicines and health supplementary foods, especially for enhancing the immune functions of the body. To extend the application of GF polysaccharides (GFP) for atopic dermatitis (AD), we investigated the effects of GFP on the 2,4-dinitrochlorobenzene-induced AD-like skin lesion in NC/Nga mice. GFP treatment significantly reduced the dorsa skin dermatitis score and combination treatment with GFP, and dexamethasone has a synergistic effect in AD-like skin lesion by reduced Serum IgE, mast cells infiltration, and cytokines expression. These results indicate that GFP suppressed the AD-like skin lesions by controlling the Th-1/Th-2-type cytokines in NC/Nga mice. These findings strongly suggest that GFP can be useful for AD patients as a novel therapeutic agent and might be used for corticosteroids replacement or supplement agent.</P>
Residue analysis of multi-class pesticides in watermelon by LC-MS/MS
Park, Semin,Lee, Sung Joong,Kim, Hae Gyeong,Jeong, Won Young,Shim, Jae-Han,Abd El-Aty, A. M.,Jeong, Sung Woo,Lee, Won Sup,Kim, Soo Taek,Shin, Sung Chul WILEY-VCH Verlag 2010 Journal of Separation Science Vol.33 No.4
<P>As watermelon is farmed primarily by protected and successive cultivation techniques, a number of pesticides are required for the control of pests and diseases. To evaluate the harmful effects of pesticides in watermelon and to guarantee consumers' safety, a rapid screening process for pesticides is required. A LC-MS/MS method was applied for the direct quantitation of 44 pesticide residues in watermelon. A Zorbax XDB-C<SUB>18</SUB> column was selected for analysis, with a mobile phase consisting of a gradient system of water and 5 mM methanolic ammonium formate. MS/MS experiments were performed in ESI positive ion and multiple reaction monitoring modes. The LOQs were in the range of 1–26 μg/kg, thereby indicating good sensitivity. Most of the recoveries ranged between 70–131% with RSDs≤20%. We suggested that the amount of pesticide residues such as pyroquilon (pyn), boscalid (bd), and dimethomorph (di) in amides (AM) and cinosulfuron (ci) in ureas (UR) may have been overestimated for the pesticides owing to increased α-error risk, whereas the amounts of pesticide residues, such as imibenconazole (ie) in the triazoles (TR) and fenpyroximate (fee) in the imidazoles (IM), may have been underestimated as the result of increased β-error risk. The current method allowed for the rapid quantitation and identification of low pesticide levels in the watermelon samples. No pesticide residues were detected in any of the surveyed watermelons obtained from eight local markets in the Republic of Korea. Statistical analysis of the recoveries classified the 44 pesticides into nine groups and three overall categories.</P>
Park, Chan Woo,Moon, Yu Gyeong,Seong, Hyejeong,Jung, Soon Won,Oh, Ji-Young,Na, Bock Soon,Park, Nae-Man,Lee, Sang Seok,Im, Sung Gap,Koo, Jae Bon American Chemical Society 2016 ACS APPLIED MATERIALS & INTERFACES Vol.8 No.24
<P>We demonstrate a new patterning technique for gallium-based liquid metals on flat substrates, which can provide both high pattern resolution (similar to 20 mu m) and alignment precision as required for highly integrated circuits. In a very similar manner as in the patterning of solid metal films by photolithography and lift-off processes, the liquid metal layer painted over the whole substrate area can be selectively removed by dissolving the underlying photoresist layer, leaving behind robust liquid patterns as defined by the photolithography. This quick and simple method makes it possible to integrate fine-scale interconnects with preformed devices precisely, which is indispensable for realizing monolithically integrated stretchable circuits. As a way for constructing stretchable integrated circuits, we propose a hybrid configuration composed of rigid device regions and liquid interconnects, which is constructed on a rigid substrate first but highly stretchable after being transferred onto an elastomeric substrate. This new method can be useful in various applications requiring both high-resolution and precisely aligned patterning of gallium-based liquid metals.</P>
Usefulness of Skin Explants for Histologic Analysis after Fractional Photothermolysis
( Gyeong Hun Park ),( Sung Eun Chang ),( Seunghyun Bang ),( Kwang Hee Won ),( Chong Hyun Won ),( Mi Woo Lee ),( Jee Ho Choi ),( Kee Chan Moon ) 대한피부과학회 2015 Annals of Dermatology Vol.27 No.3
Background: Fractional laser resurfacing treatment has been extensively investigated and is widely used. However, the mechanism underlying its effects is poorly understood because of the ethical and cosmetic problems of obtaining skin biopsies required to study the changes after laser treatment. Objective: To evaluate the usefulness of human skin explants for the investigation of fractional photothermolysis. Methods: Full-thickness discarded skin was treated in 4 ways: no treatment (control), fractional carbon dioxide laser, fractional Er:YAG laser, and fractional 1,550-nm erbium- doped fiber laser. Both treated and non-treated skin samples were cultured ex vivo at the air-medium interface for 7 days. Frozen tissue was sectioned and stained with hematoxylin & eosin for histologic examination and nitro blue tetrazolium chloride for viability testing. Results: Skin explants cultured for up to 3 days exhibited histologic changes similar to those observed in in vivo studies, including microscopic treatment zones surrounded by a thermal coagulation zone, re-epithelialization, and formation of microscopic epidermal necrotic debris. However, the explant structure lost its original form within 7 days of culture. The viability of skin explants was maintained for 3 days of culture but was also lost within 7 days. Conclusion: The skin explant model may be a useful tool for investigating the immediate or early changes following fractional photothermolysis, but further improvements are required to evaluate the long-term and dermal changes. (Ann Dermatol 27(3) 283∼290, 2015)