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Wesley Lim,Ian Phau,Isaac Cheah,Min Teah 글로벌지식마케팅경영학회 2015 Global Fashion Management Conference Vol.2015 No.06
This paper seeks to conceptualize the phenomenon known as the “Fear of Missing Out” by developing and validating a measurement scale – “Fear of Missing Out” (FOMO-SCALE) to be used in a marketing context. It will also further explore various antecedents that may impact on FOMO. The study suggests an alternative approach towards the conceptualization of FOMO by defining it as a personality trait as opposed to an outcome of a behavior.
Controlled Lysis of Escherichia coli Double - Lysogen of Bacteriophages λHL1 and φ434
Koo, Yoon Mo,Lim, Henry C,Parekh, Bhavin S,Hatfield, G Wesley 한국화학공학회 1996 NICE Vol.14 No.3
A novel phage double-lysogen was developed to produce an intracellular protein and disrupt the host cell in the same reactor. Using this double-lysogen, we could simplify the recovering processes without cell harvest and, disruption. Construction of the doutrle-lysogen is based on the fact that a lysogen of a phage can be superinfected by another phage with different immunity. Thc single-lysogen of Escherichia coli, P90c/λHL1, was superinfected with bacteriophage Ø434 to produce a double-lysogen, in which phage genomes from each phage coexisted in the host chromosome. Two different inducers were used to induce the double-lysogen to producx a protein and to lyse the host cell. The first phage gercome, λHL1, the prophage of the original lysogen, containing the temperature sensitive cles, lacZ and defective Q genes was induced by increasing temperature to produce p-galactosidase, an intracellular reporter proteitc. The overproduction of 3-galactosidase was carried out without experiencing the cell lysis due to the defective Q gene. After the temperature shift, the second praphage from the lysogen MS21/Ø434 was induced by mitomycin C or ultra-violet light to lyse the cell. The lysis of the cell releases the intracellular protein to the outer space. The cell lysis was confirmed by the decrease of cell density and the increasev of the extracxllular activity of galactosidase at the same time.
CONTROLLED LYSIS OF ESCHERICHIA COLI DOUBLE - LYSOGEN OF BACTERIOPHAGES λHL1 AND φ434
Koo, Yoon Mo,Parekh, Bhavin S,Hatfield, G Wesley,Lim, Henry C 한국화학공학회 1996 Korean Journal of Chemical Engineering Vol.13 No.2
A novel phage double-lysogen was developed to produce an intracellular protein and disrupt the host cell in the same reactor. Using this double-lysogen, we could simplify the recovering processes without cell harvest and disruption. Construction of the double-lysogen is based on the fact that a lysogen of a phage can be superinfected by another phage with different immunity. The single-lysogen of Escherichia coli, P90c/λHL1, was superinfected with bacteriophage Φ434 to produce a double-lysogen, in which phage genomes from each phage coexisted in the host chromosome. Two different inducers were used to induice the double-lysogen to produce a protein and to lyse the host cell. The first phage genome, λHL1, the prophage of the original lysogen, containing the temperature sensitive cI_(857), lacZ and defective Q genes was induced by increasing temperature to produce β-galactosidase, an intracellular reporter protein. The overproduction of β-galactosidase was carried out without experiencing the cell lysis due to the defective Q gene. After the temperature shift, the second prophage from the lysogen MS21/Φ434 was induced by mitomycin C or ultra-violet light to lyse the cell. The lysis of the cell releases the intracellular protein to the outer space. The cell lysis was confirmed by the decrease of cell density and the increase of the extracellular activity of β-galactosidase at the same time.