Observer-based Controller Design for A T-S Fuzzy System with Unknown Premise Variables

Wen-Bo Xie,He Li,Zhenhua Wang,Jian Zhang 제어·로봇·시스템학회 2019 International Journal of Control, Automation, and Vol.17 No.4

For the stabilization problem of T-S fuzzy system, a new observer-based controller design approachis proposed when premise variables are not accessible. With a fuzzy observer, the estimated states error system isdescribed as two parts: unknown premise variable caused terms and observer error terms. Consider the property thatthe norm of the unknown premise variable caused terms are under a Lipschitz condition constraint of observer error,an observer and controller errors augmented system is obtained. Then based on the Lyapunov function method, aseries of linear matrix inequality conditions are proposed to asymptotically stabilize the system, the observer gainmatrices are used to overcome the uncertainties caused by UPVs. Finally a simulation example is used to illustratethe effectiveness of the proposed method, comparisons with traditional method shows the conservatism reductioneffects.

Steel Bridge Construction of Hong Kong–Zhuhai–Macao Bridge

Wen-bo Gao,Quan-ke Su,Jin-wen Zhang,Hong-bing Xie,Feng Wen,Fang Li,Ji-zhu Liu 한국강구조학회 2020 International Journal of Steel Structures Vol.20 No.5

The 55-km-long Hong Kong–Zhuhai–Macao Bridge (HZMB) is the world’s longest sea-crossing bridge, connecting Hong Kong with Zhuhai and Macao at the mouth of the Pearl River Estuary in China, comprising 22.9-km-long steel bridges. HZMB is the leading steel bridge in China, with top-level manufacturing and installation technology. This paper outlines the steel bridge construction experiences of HZMB to provide comparisons for the construction of other long sea-crossing steel bridges at home or abroad. The main considerations of construction constraints, scheme selection, structural and aesthetic design of HZMB are presented, and the following points related to new strategies in the steel bridge construction of HZMB are elaborated: (1) construction quality assurance, (2) automatic manufacturing technology, (3) large segment off shore installation, (4) eco-friendly paint (content limitation of volatile organic compounds) and new multifunctional inspection gantry, and (5) Guss Mastic Asphalt steel deck pavement system. The successful implementation of those strategies shows that the steel bridge construction of HZMB promotes improvement in the overall construction and management level of the Chinese bridge industry. The advanced experience of HZMB has opened up broad prospects for the design and construction of off shore bridge engineering in China.

Copper as an Antimicrobial Agent against Opportunistic Pathogenic and Multidrug Resistant Enterobacter Bacteria

Wen-Xiao Tian,Shi Yu,Muhammad Ibrahim,Abdul Wareth Almonaofy,Liu He,Qiu Hui,Zhu Bo,Bin Li,Guan-lin Xie 한국미생물학회 2012 The journal of microbiology Vol.50 No.4

Infections by Enterobacter species are common and are multidrug resistant. The use of bactericidal surface materials such as copper has lately gained attention as an effective antimicrobial agent due to its deadly effects on bacteria,yeast, and viruses. The aim of the current study was to assess the antibacterial activity of copper surfaces against Enterobacter species. The antibacterial activity of copper surfaces was tested by overlying 5×106 CFU/ml suspensions of representative Enterobacter strains and comparing bacterial survival counts on copper surfaces at room temperature. Iron, stainless steel, and polyvinylchloride (PVC) were used as controls. The mechanisms responsible for bacterial killing on copper surfaces were investigated by a mutagenicity assay of the D-cycloserin (cyclA gene), single cell gel electrophoresis,a staining technique, and inductively coupled plasma mass spectroscopy. Copper yielded a significant decrease in the viable bacterial counts at 2 h exposure and a highly significant decrease at 4 h. Loss of cell integrity and a significantly higher influx of copper into bacterial cells exposed to copper surfaces, as compared to those exposed to the controls,were documented. There was no increase in mutation rate and DNA damage indicating that copper contributes to bacterial killing by adversely affecting cellular structure without directly targeting the genomic DNA. These findings suggest that copper’s antibacterial activity against Enterobacter species could be utilized in health care facilities and in food processing plants to reduce the bioburden, which would increase protection for susceptible members of the community.

Identifying Differentially Expressed Genes and Screening Small Molecule Drugs for Lapatinib-resistance of Breast Cancer by a Bioinformatics Strategy

Zhuo, Wen-Lei,Zhang, Liang,Xie, Qi-Chao,Zhu, Bo,Chen, Zheng-Tang Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.24

Background: Lapatinib, a dual tyrosine kinase inhibitor that interrupts the epidermal growth factor receptor (EGFR) and HER2/neu pathways, has been indicated to have significant efficacy in treating HER2-positive breast cancer. However, acquired drug resistance has become a very serious clinical problem that hampers the use of this agent. In this study, we aimed to screen small molecule drugs that might reverse lapatinib-resistance of breast cancer by exploring differentially expressed genes (DEGs) via a bioinformatics method. Materials and Methods: We downloaded the gene expression profile of BT474-J4 (acquired lapatinib-resistant) and BT474 (lapatinib-sensitive) cell lines from the Gene Expression Omnibus (GEO) database and selected differentially expressed genes (DEGs) using dChip software. Then, gene ontology and pathway enrichment analyses were performed with the DAVID database. Finally, a connectivity map was utilized for predicting potential chemicals that reverse lapatinib-resistance. Results: A total of 1, 657 DEGs were obtained. These DEGs were enriched in 10 pathways, including cell cycling, regulation of actin cytoskeleton and focal adhesion associate examples. In addition, several small molecules were screened as the potential therapeutic agents capable of overcoming lapatinib-resistance. Conclusions: The results of our analysis provided a novel strategy for investigating the mechanism of lapatinib-resistance and identifying potential small molecule drugs for breast cancer treatment.

State Augmented Feedback Controller Design Approach for T-S Fuzzy System with Complex Actuator Saturations

Jian Zhang,Wen-Bo Xie,Mou-Quan Shen,Ling Huang 제어·로봇·시스템학회 2017 International Journal of Control, Automation, and Vol.15 No.5

The control problem of T-S fuzzy system with actuator amplitude, rate and acceleration saturations isaddressed in this paper, where state augmented feedback controller with LMIs (Liner Matrix Inequalities) constraintconditions are proposed. Dynamic decoupling method is applied to fuzzify the input magnitude saturation nonlinearityinto several sub-linear systems with fuzzy rules, thus a new T-S fuzzy system with input rate and accelerationsaturations can be obtained. Then PDC (parallel distributed compensation) and NPDC (non-PDC) controller areboth designed for the new T-S fuzzy system. The first and second order derivatives of input variable are given toobtain an augmented fuzzy system. As the augmented system output variable, the input rate is represented by firstorder derivative of the input term, the rate saturation constraint is described through norm bounded method. Moreover,the polytopic approach is used to replace the second order derivative of the input term, and a state augmentedfeedback NPDC controller is designed, the domain of attraction optimization process is also given. Finally, twopractical examples are presented to show the effectiveness of proposed method.

Reachable Set Estimation for Uncertain Linear Systems with Known Inputs

Zhihao Zhang,Xinsheng Wang,Wen-Bo Xie,Xin Zhang,Yi Shen 제어·로봇·시스템학회 2020 International Journal of Control, Automation, and Vol.18 No.10

This paper studies a reachable set estimation for systems with parameter uncertainties and known input. A new method is proposed for estimating real-time reachable sets of systems with parameter uncertainties, known input and non-zero initial condition. In order to reduce the effect of known inputs on the reachable set estimation, we propose a method by separating the uncertain systems into two subsystems: the subsystem with known input and the subsystem with parameter uncertainties. By solving the optimization problem in terms of linear matrix inequalities, real-time reachable sets are obtained. The effectiveness of the proposed method is illustrated by the simulation result of the lateral dynamic system of a vehicle.

Suppression of Ku80 Correlates with Radiosensitivity and Telomere Shortening in the U2OS Telomerase-negative Osteosarcoma Cell Line

Hu, Liu,Wu, Qin-Qin,Wang, Wen-Bo,Jiang, Huan-Gang,Yang, Lei,Liu, Yu,Yu, Hai-Jun,Xie, Cong-Hua,Zhou, Yun-Feng,Zhou, Fu-Xiang Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.2

Ku70/80 heterodimer is a central element in the nonhomologous end joining (NHEJ) DNA repair pathway, Ku80 playing a key role in regulating the multiple functions of Ku proteins. It has been found that the Ku80 protein located at telomeres is a major contributor to radiosensitivity in some telomerase positive human cancer cells. However, in ALT human osteosarcoma cells, the precise function in radiosensitivity and telomere maintenance is still unknown. The aim of this study was to investigate the effects of Ku80 depletion in the U2OS ALT cell line cell line. Suppression of Ku80 expression was performed using a vector-based shRNA and stable Ku80 knockdown in cells was verified by Western blotting. U2OS cells treated with shRNA-Ku80 showed lower radiobiological parameters (D0, Dq and SF2) in clonogenic assays. Furthermore, shRNA-Ku80 vector transfected cells displayed shortening of the telomere length and showed less expression of TRF2 protein. These results demonstrated that down-regulation of Ku80 can sensitize ALT cells U2OS to radiation, and this radiosensitization is related to telomere length shortening.