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Nonthaneth Nalinratana,Weeraya Kaewprem,Sarunya Tongumpai,Rataya Luechapudiporn,Uthai Sotanaphun,Duangdeun Meksuriyen 한국한의학연구원 2014 Integrative Medicine Research Vol.3 No.2
Background: Phikud Navakot (PN), a combination of nine herbs, has been used traditionally inThai medicinal formulas to relieve circulatory disorder. The present study aimed to comparethe synergistic antioxidant efficacy and toxicity of the hydroethanolic and water extracts ofPN at cellular level.Methods: PN and its nine herbs were extracted with either 50% ethanol or water. All extractswere tested for in vitro antioxidant potential using standard antioxidant assays. Evaluationof cytotoxicity, genotoxicity, and intracellular reactive oxygen species were performed usinghuman endothelial ECV304 cells.Results: Antioxidant assays in cell-free systems showed that the hydroethanolic extractof PN scavenged superoxide, hydroxyl, nitric oxide radicals, and hydrogen peroxide moreeffectively than its water extract. Combination indices were calculated to show that theingredients of the hydroethanolic extract acted synergistically to exhibit antioxidant activi-ties against all tested radicals, whereas, in the case of water extract, this effect was observedonly against 2,2-diphenyl-1-picrylhydrazyl, superoxide, and hydroxyl radicals. A cell-basedassay also revealed that the hydroethanolic extract concentration-dependently attenuatedhydrogen peroxide-induced stress more effectively than the water extract. At the antioxi-dant and cytotoxic concentrations of both extracts, no genotoxicity was found.Conclusion: Our findings demonstrate that the synergistic antioxidant action of PN amelio-rates endothelial stress, which may provide some clues for understanding the traditionaluse of PN for the treatment of circulatory disorder. Additionally, the selection of a suitablesolvent for the extraction of PN herbal combination is essential for maximal efficacy andsafety