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Kim, Wanhui,Kim, Hee-Eun,Jun, A Rim,Jung, Myeong Gyo,Jin, Suhyun,Lee, Joon-Hwa,Ahn, Ji Hoon Oxford University Press 2016 Journal of experimental botany Vol.67 No.15
<P>MicroRNAs originate from primary transcripts (pri-miRNAs) containing hairpin structures. Plant pri-miRNAs have highly variable structures and little is known about the information encoded in their secondary structures. Arabidopsis miR156 is an ambient temperature-responsive miRNA and plays an important role in regulating flowering time. To identify the structural determinants for miR156 processing, we analyzed the effects of mutations introduced in the upper stem of pri-miR156a on its temperature-dependent processing and flowering time. The levels of pri-miR156a and mature miR156 were opposite at different temperatures. Mutations in the upper stem, especially the region closer to the miR156a/miR156a* duplex, reduced miR156 processing at 23 °C and 16 °C and caused a less severe phenotype compared with the un-mutated construct. Mutation in the second stem near the first cleavage site of pri-miR156a affected miR156 processing at 23 °C, but not at 16 °C. This was also seen in pri-miR172a, another ambient temperature-responsive miRNA. Replacement of the upper stem of pri-miR156a with that of pri-miR172a severely affected miR156 processing and flowering time. These results suggested that the upper stem of pri-miR156a is important for miR156 processing at different temperatures. In particular, the second stem adjacent to the first cleavage site plays a role in the regulation of ambient temperature-responsive flowering.</P>
Kim, Wanhui,Kim, Hee-Eun,Lee, Ae-Ree,Jun, A Rim,Jung, Myeong Gyo,Ahn, Ji Hoon,Lee, Joon-Hwa Oxford University Press 2017 Nucleic acids research Vol.45 No.2
<P>MicroRNAs originate from primary transcripts containing hairpin structures. The levels of mature miR156 influence the leaf number prior to flowering in the life cycle of plants. To understand the molecular mechanism of biogenesis of primary miR156a (pri-miR156a) to mature miR156, a base-pair opening dynamics study was performed using model RNAs mimicking the cleavage site of wild type and B5 bulge-stabilizing mutant pri-miR156a constructs. We also determined the mature miR156 levels and measured leaf numbers at flowering of plants overexpressing the wild type and mutant constructs. Our results suggest that the stabilities and/or opening dynamics of the C15·G98 and U16·A97 base-pairs at the cleavage site are essential for formation of the active conformation and for efficient processing of pri-miR156a, and that mutations of the B5 bulge can modulate mature miR156 levels as well as miR156-driven leaf number phenotypes via changes in the base-pair stability of the cleavage site.</P>
陈万会 청주대학교 국제협력연구원 2005 國際文化硏究 Vol.23 No.-
语言接触属于语言关系范疇, 涉及语言结构特点、语言功能以及语言习得等问题。偏误分析从理论上来说, 可以帮助我们发现语言习得现律幷深化我们的认识; 从实践上说, 有助于预测和避免错误, 以指导教学。在对韩国学生的汉语教学中, 我们应当自觉利用语言接触和偏误分析理论, 帮助他们发挥汉字优势, 克服语际语內干扰, 以提高教学效果。 Language contact involves language structure, language function, and language acquisition. Error analysis aims to find the rules and regularities of the errors in foreign language acquisition and thus provide ways to improve foreign languageteaching and learning. Error analysis of the Korean students in their study of Chinese as a foreign language can help to improve their study in a more efficient way.
Hyelim Jeon,Wanhui Kim,Boyoung Kim,Sookyeong Lee,Jay Jayaraman,Gayoung Jung,Sera Choi,Kee Hoon Sohn,Cé,cile Segonzac 한국식물병리학회 2020 Plant Pathology Journal Vol.36 No.1
Ralstonia solanacearum (Rso) is a causal agent of bacterial wilt in Solanaceae crops worldwide including Republic of Korea. Rso virulence predominantly relies on type III secreted effectors (T3Es). However, only a handful of Rso T3Es have been characterized. In this study, we investigated subcellular localization of and manipulation of plant immunity by 8 Rso T3Es predicted to harbor a nuclear localization signal (NLS). While 2 of these T3Es elicited cell death in both Nicotiana benthamiana and N. tabacum, only one was dependent on suppressor of G2 allele of skp1 (SGT1), a molecular chaperone of nucleotide-binding and leucine-rich repeat immune receptors. We also identified T3Es that differentially regulate flg22-induced reactive oxygen species production and gene expression. Interestingly, several of the NLS-containing T3Es translationally fused with yellow fluorescent protein accumulated in subcellular compartments other than the cell nucleus. Our findings bring new clues to decipher Rso T3E function in planta.
Kim, Hee-Eun,Kim, Wanhui,Lee, Ae-Ree,Jin, Suhyun,Jun, A.Rim,Kim, Nak-Kyoon,Lee, Joon-Hwa,Ahn, Ji Hoon Academic Press 2017 Biochemical and biophysical research communication Vol. No.
<P><B>Abstract</B></P> <P>Internal and environmental cues, including ambient temperature changes, regulate the timing of flowering in plants. Arabidopsis miR156 represses flowering and plays an important role in the regulation of temperature-responsive flowering. However, the molecular basis of miR156 processing at lower temperatures remains largely unknown. Here, we performed nuclear magnetic resonance studies to investigate the base-pair opening dynamics of model RNAs at 16 °C and investigated the <I>in vivo</I> effects of the mutant RNAs on temperature-responsive flowering. The A9C and A10CG mutations in the B5 bulge of the lower stem of pri-miR156a stabilized the C15∙G98 and U16∙A97 base-pairs at the cleavage site of pri-miR156a at 16 °C. Consistent with this, production of mature miR156 was severely affected in plants overexpressing the A9C and A10CG constructs and these plants exhibited almost no delay in flowering at 16 °C. The A10G and A9AC mutations did not strongly affect C15∙G98 and U16∙A97 base-pairs at 16 °C, and plants overexpressing A10G and A9AC mutants of miR156 produced more mature miR156 than plants overexpressing the A9C and A10CG mutants and showed a strong delay in flowering at 16 °C. Interestingly, the A9AC mutation had distinct effects on the opening dynamics of the C15∙G98 and U16∙A97 base-pairs between 16 °C and 23 °C, and plants expressing the A9AC mutant miR156 showed only a moderate delay in flowering at 16 °C. Based on these results, we propose that fine-tuning of the base-pair stability at the cleavage site is essential for efficient processing of pri-miR156a at a low temperature and for reduced flowering sensitivity to ambient temperature changes.</P> <P><B>Highlights</B></P> <P> <UL> <LI> A mutation in pri-miR156a B5 affected base-pairing stability at the cleavage site. </LI> <LI> Mutations in the B5 bulge increased base-pair stabilities and decreased miR156. </LI> <LI> The A9AC mutation affected opening dynamics between 16 and 23 °C. </LI> <LI> Cleavage site base-pair stabilities reflected miR156 levels and flowering time. </LI> </UL> </P>
Pirah Ayaz,Jie Li,Wanhui Jin,Mingbo Ma 한국섬유공학회 2020 Fibers and polymers Vol.21 No.3
The pigment of naturally colored domestic silk (NCDS) is present in the sericin layer rather than in the silk fibroin,and the color losses easily during the wet process accompanied by dissolution of the sericin. In this study, the sericin ofnaturally colored domestic silk was cross-linked to a water-insoluble network via treatment with a hydroxyalkyl phosphinecompound, tetrakis (hydroxymethyl) phosphonium sulfate (THPS). The effects of reaction conditions, such as pH, reagentconcentration, reaction temperature, and time on the cross-linking effect were investigated. Cross-linking the NCDS samplesat a relatively good condition could decrease the weight loss from initial 16.7 % to less than 4.5 % during degumming. Thecross-linked NCDS showed a K/S value (color depth) 1.84 times higher than the uncross-linked sample after 20 cycles ofwashing. The method for fixing the color of NCDS offers a kind of solution with high practicality and low cost. The crosslinkerproposed in this study could also be an alternative for cross-linking sericin based materials.