Unleashing the power of diatomaceous earths for sustainable management of Tribolium castaneum infestation in wheat

Wakil Waqas,Kavallieratos Nickolas G.,Eleftheriadou Nikoleta,Riasat Tahira,Rasool Khawaja G.,Husain Mureed,Aldawood Abdulrahman S. 한국응용곤충학회 2023 Journal of Asia-Pacific Entomology Vol.26 No.4

The utilization of diatomaceous earths (DEs) has been considered as a potential alternative to traditional in secticides for controlling grain storage insect pests. In this study, 19 different commercial DEs obtained from various regions of the world were evaluated for their effectiveness to manage Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae), in vitro. Two different series of bioassays were carried out, with the first screening all 19 DEs and the second evaluating the eight most effective ones at different doses and exposure intervals for mortality assessment and progeny production. Four enhanced DEs, namely InertPMS, DEA, DiaHerb, and DEBBM were highly effective compared to other DEs tested. InertPMS exhibited complete adult mortality at 50 mg/kg, followed by DEA at 75 mg/kg, DiaHerb at 100 mg/kg, and DEBBM at 125 mg/kg, while GrainGuard exhibited 94.52 % at 125 mg/kg, 14 days post-exposure. Protect-It, SilicoSec, and DiaFil 610 were less effective at 400 mg/ kg, causing 87.3 %, 84.4 %, and 79.2 % mortality after 14 days, respectively. The offspring was suppressed in wheat treated with InertPMS at 75 mg/kg, while DEA, DiaHerb, and DEBBM revealed no progeny emergence at 125 mg/kg. GrainGuard, Protect-It, SilicoSec, and DiaFil 610 were not successful at progeny suspension after 62 days. These findings suggest that the use of specific DEs at low doses could be a viable option to mitigate the use of conventional insecticides and diminish the residues of insecticides on stored grains, making them the most feasible components for integrated pest management (IPM) in stored grain systems.

Accurate measurement of pressure differences and the effect of baffle on pressure distribution in vacuum chamber during dynamic gas flow

Wakil Khan,I.M. Choi,J.Y. Lim,S.S. Hong 한국물리학회 2010 Current Applied Physics Vol.10 No.2

The need to know the pressure in relatively large vacuum chambers arises at different measurements (calibration of vacuum gauges, outgassing measurements, etc.) and investigating the ways to minimize these pressure differences are of equal importance in such industrial as well as research applications. The experimental determination of the pressure differences between various gauge positions in a chamber of cylindrical shape and the effect of a baffle on pressure distribution are described in the paper. The pressure in the chamber was generated dynamically in the pressure range 0.1–133 Pa through adjustable gas flow into the chamber. Five identical capacitance diaphragm gauges (CDGs) were used in which one CDG remained fixed at the central position of the chamber while the other four (in two pairs) were mutually interchanged. The differences on the order of several percent between positions at different levels were calculated and the effect of the baffle was investigated which are specific for this chamber.

A Study of Non-uniform Pressure Distribution in Vacuum Chamber during Dynamic Gas Flow

Wakil Khan(와킬 칸),K. S. Hong(홍기성),S. S. Hong(홍승수) 한국진공학회(ASCT) 2009 Applied Science and Convergence Technology Vol.18 No.6

진공챔버는 진공게이지 교정, 산업에서의 재료처리 등 여러 가지 다양한 용도에 맞게 적용이 가능하다. 이 진공챔버 내부에서 가스가 유입되는 과정에서의 진공도는 일정하게 유지하기가 힘들다. 산업체 응용에서뿐만 아니라 연구과정에서도 진공챔버 내부에 가스가 유입되는 동안의 내부압력분포와 최대도달 평형압력을 아는 것이 매우 중요하다. 이러한 진공챔버 내부의 압력 불균형을 감소시키기 위해서 가스 주입구 부분에 baffle을 이용하는 방법이 있다. 현재 지속적인 기체흐름이 있는 진공챔버 내부의 기체흐름의 작용에 관해 0.1 Pa~133 Pa 영역에서 불규칙한 압력을 최소화하기 위한 baffle plate의 효과에 대해 연구하였다. 최대편차는 가스 주입구 부분에서 나타나는 압력으로 baffle plate가 전환흐름영역에서 큰 영향을 미치는 것으로 나타났다. Vacuum chambers have wide application for a variety of purposes such as material processing, vacuum gauge calibration, etc. As the dynamic pressure generated in such chamber is non-uniform, in many industrial as well as research processes, it is vital to know the non-uniform gas distribution with associated gas flow regimes and the ways of minimizing these pressure non-uniformities. In the present work, the behavior of gas flow in a vacuum chamber, during continuous gas flow, is described in the pressure range 0.1-133 Pa and the effect of baffle plate in minimizing the pressure non-uniformities is investigated. It was observed that maximum deviations in the pressure occur near the gas inlet point and that the effect of baffle plate in minimizing the pressure non-uniformities is more obvious in the transitional flow regime.

Comparative Analyses for Aroma and Agronomic Traits of Native Rice Cultivars from Central Asia

Wakil Ahmad SARHADI,Nguyen Loc HIEN,Mehran ZANJANI,Wahida YOSOFZAI,Tadashi YOSHIHASHI,Yutaka HIRATA 한국작물학회 2008 Journal of crop science and biotechnology Vol.11 No.1

Aromatic rice has become popular owing to its aroma. Growing demand for aromatic rice has spurred interest in the development of domestic cultivars that offer similar combinations of grain attributes such as texture, cooking characteristics, aroma, and taste. In this study, the most important agronomic attributes and aroma of 26 cultivars from Afghanistan, Iran, and Uzbekistan, and controls from Japan, Thailand, and India were characterized. Also F2 populations derived from the cross between (Jasmine 85 aromatic x Nipponbare non-aromatic) and (Jasmine 85 x Basmati 370 aromatic) were obtained. Tasting individual grains, cooking test, 1.7% KOH sensory test, and molecular marker analysis have been applied to distinguish between aromatic and non-aromatic rice. Diversity for some traits of agronomic importance, such as plant height was detected among countries, e.g. Afghan cultivars classified as tall, and Iranian and Uzbek intermediate and short, respectively. Differentiations of panicle, grain, leaf, basal internode, and culm dimension among rice cultivars, indicating the source of rice diversity in Central Asia. According to the results, 6 of 10, 2 of 7, and 0 of 6 of Afghan, Iranian, and Uzbek rice cultivars were scored as aromatic, respectively. Therefore, Afghan cultivars are a good source of aromatic rice germplasm for Central Asia. The expression between aromatic and non-aromatic, and aromatic and aromatic combinations has been evaluated. The observed segregation ratio of these crosses in the F2 populations was tested by χ2 analysis against the expected ratio for a single gene. A segregation ratio of 3:1 between non-aromatic and aromatic combination has been detected, while segregation has not been detected between the aromatic and aromatic combinations. Also, parallel results were obtained from the tested aromatic rice cultivars. Thus, our results suggest that a single recessive gene controls aroma in all aromatic rice cultivars. Aromatic rice has become popular owing to its aroma. Growing demand for aromatic rice has spurred interest in the development of domestic cultivars that offer similar combinations of grain attributes such as texture, cooking characteristics, aroma, and taste. In this study, the most important agronomic attributes and aroma of 26 cultivars from Afghanistan, Iran, and Uzbekistan, and controls from Japan, Thailand, and India were characterized. Also F2 populations derived from the cross between (Jasmine 85 aromatic x Nipponbare non-aromatic) and (Jasmine 85 x Basmati 370 aromatic) were obtained. Tasting individual grains, cooking test, 1.7% KOH sensory test, and molecular marker analysis have been applied to distinguish between aromatic and non-aromatic rice. Diversity for some traits of agronomic importance, such as plant height was detected among countries, e.g. Afghan cultivars classified as tall, and Iranian and Uzbek intermediate and short, respectively. Differentiations of panicle, grain, leaf, basal internode, and culm dimension among rice cultivars, indicating the source of rice diversity in Central Asia. According to the results, 6 of 10, 2 of 7, and 0 of 6 of Afghan, Iranian, and Uzbek rice cultivars were scored as aromatic, respectively. Therefore, Afghan cultivars are a good source of aromatic rice germplasm for Central Asia. The expression between aromatic and non-aromatic, and aromatic and aromatic combinations has been evaluated. The observed segregation ratio of these crosses in the F2 populations was tested by χ2 analysis against the expected ratio for a single gene. A segregation ratio of 3:1 between non-aromatic and aromatic combination has been detected, while segregation has not been detected between the aromatic and aromatic combinations. Also, parallel results were obtained from the tested aromatic rice cultivars. Thus, our results suggest that a single recessive gene controls aroma in all aromatic rice cultivars.

A Study of Non-uniform Pressure Distribution in Vacuum Chamber during Dynamic Gas Flow

Wakil Khan,K. S. Hong,I. T. Lim,Y. H. Shin,S. S. Hong 한국진공학회 2009 한국진공학회 학술발표회초록집 Vol.37 No.-

Comparative Analyses for Aroma and Agronomic Traits of Native Rice Cultivars from Central Asia

Sarhadi, Wakil Ahmad,Hien, Nguyen Loc,Zanjani, Mehran,Yosofzai, Wahida,Yoshihashi, Tadashi,Hirata, Yutaka The Korean Society of Crop Science 2008 Journal of crop science and biotechnology Vol.11 No.1

Aromatic rice has become popular owing to its aroma. Growing demand for aromatic rice has spurred interest in the development of domestic cultivars that offer similar combinations of grain attributes such as texture, cooking characteristics, aroma, and taste. In this study, the most important agronomic attributes and aroma of 26 cultivars from Afghanistan, Iran, and Uzbekistan, and controls from Japan, Thailand, and India were characterized. Also $F_2$ populations derived from the cross between(Jasmine 85 aromatic$\times$Nipponbare non-aromatic) and(Jasmine 85$\times$Basmati 370 aromatic) were obtained. Tasting individual grains, cooking test, 1.7% KOH sensory test, and molecular marker analysis have been applied to distinguish between aromatic and non-aromatic rice. Diversity for some traits of agronomic importance, such as plant height was detected among countries, e.g. Afghan cultivars classified as tall, and Iranian and Uzbek intermediate and short, respectively. Differentiations of panicle, grain, leaf, basal internode, and culm dimension among rice cultivars, indicating the source of rice diversity in Central Asia. According to the results, 6 of 10, 2 of 7, and 0 of 6 of Afghan, Iranian, and Uzbek rice cultivars were scored as aromatic, respectively. Therefore, Afghan cultivars are a good source of aromatic rice germplasm for Central Asia. The expression between aromatic and non-aromatic, and aromatic and aromatic combinations has been evaluated. The observed segregation ratio of these crosses in the $F_2$ populations was tested by $x^2$ analysis against the expected ratio for a single gene. A segregation ratio of 3:1 between non-aromatic and aromatic combination has been detected, while segregation has not been detected between the aromatic and aromatic combinations. Also, parallel results were obtained from the tested aromatic rice cultivars. Thus, our results suggest that a single recessive gene controls aroma in all aromatic rice cultivars.

Comparative Efficacy of Different Grain Protectants Against Tribolium castaneum Under Two Sets of Temperature and Humidity

Waqas Wakil,Mansoor-ul-Hasan,Faiza Bashir,Muhammad Sagheer 한국응용곤충학회 2005 한국응용곤충학회 학술대회논문집 Vol.2005 No.05

Trends in integrated pest management strategies for the control of Helicoverpa armigera (Hübner) caterpillars on chickpea (Cicer arietinum L.)

Waqas WAKIL,Muhammad ASHFAQ,권용정,M. U. GHAZANFAR 한국곤충학회 2009 Entomological Research Vol.39 No.1

A study was carried out at the University of Arid Agriculture, Rawalpindi, Pakistan to integrate different control measures against Helicoverpa armigera (Hübner) on chickpea, to minimize the use of hazardous insecticides and develop an eco-friendly management program. Cultural (weeding), mechanical (hand picking), biological (release of Trichogramma chilonis), microbial (spraying of Bacillus thruingiensis) and chemical (Steward) control practices were applied three times at 10 day intervals individually and also in various combinations. The application of Steward proved most effective when applied alone, with 0.41 larvae/plant, pod infestation of 9.31% and the highest grain yield (1203.66 g/plot); however, the integration of weeding, hand picking and Steward proved to be the most effective in reducing the larval population (0.12 larvae/plant) with minimum pod infestation (5.45%) on variety CM-2000, which resulted in the maximum grain yield (1260.33 g/plot). The cost–enefit ratio in the treatment where Steward was applied alone was 1:2.20, and it was 1:3.53 where weeding and hand picking practices were integrated.

Effect of modified meridic diet on the development and growth of tomato fruitworm Helicoverpa armigera (Lepidoptera: Noctuidae)

Waqas Wakil,M. Usman GHAZANFAR,Shahbaz Talib Sahi,권용정,M. Abdul Qayyum 한국곤충학회 2011 Entomological Research Vol.41 No.3

The efficacy of one new modified and two old meridic diets on Helicoverpa armigera Hübner (Lepidoptera: Noctuidae) for rearing six successive generations was studied. Duration of larval development for insects fed on the modified diet was considerably shortened as most of them went through only five stadia before pupation, while the per cent pupation and per cent eclosion were relatively higher than on other diets. The lowest pupal mortality (6.33 ± 0.13%) was recorded in the F1 generation reared on the modified diet, whereas the highest pupal mortality (19.49 ± 0.15%) was observed in insects reared on a natural diet in the F6 generation. Blending of chickpea Cicer arietinum L. and red kidney bean Phaseolus vulgaris L. flours with tomato paste proved highly favorable for adult reproduction. These results suggest that the vitality of the tomato fruitworm did not decline obviously after rearing on a modified diet for several generations.

Occurrence and diversity of entomopathogenic fungi in cultivated and uncultivated soils in Pakistan

Waqas Wakil,M. Usman GHAZANFAR,Tahira RIASAT,권용정,Mirza Abdul QAYYUM,Muhammad YASIN 한국곤충학회 2013 Entomological Research Vol.43 No.1

The distribution of entomopathogenic fungi in various geographical areas of Punjab, Pakistan, is poorly understood. The present study was planned to explore the occurrence and diversity of entomopathogenic fungi from soils collected from cultivated and non‐cultivated habitats. The detailed survey of different habitats (crop fields, fruits, vegetables, forests) was conducted to collect soil samples and the associated fungi were isolated using Galleria bait method. Among 210 soil samples, 168 fungal isolates were recovered and identified, with 98 from forests, 32 from vegetables, 30 from field crops and 8 from fruits. The major entomopathogenic fungi recovered from these samples were Beauveria bassiana, Metarhizium anisopliae, Paecilomyces lilacinus, B. brongniartii, P. chlamydosporia and Lecanicillium attenuatum. The diversity of entomopathogenic fungi was greater in soil samples from forests compared to crop fields, vegetables and fruits, respectively. The geographical attributes (altitude, longitude, latitude) greatly influenced the occurrence of entomopathogenic fungi with the highest number of isolates found from >600 m altitude, 33°–34′N latitude, and 73°74′E longitude from soil samples. The results of the surveys clearly indicated that the entomopathogenic fungi are distributed in the soils which may be used in successful IPM programs in future.