Actin-mediated movement of chloroplasts

Wada, Masamitsu,Kong, Sam-Geun The Company of Biologists Ltd. 2018 Journal of cell science Vol.131 No.2

<P>Plants are sessile and require diverse strategies to adapt to fluctuations in the surrounding light conditions. Consequently, the photorelocation movement of chloroplasts is essential to prevent damages that are induced by intense light (avoidance response) and to ensure efficient photosynthetic activities under weak light conditions (accumulation response). The mechanisms that underlie chloroplast movements have been revealed through analysis of the behavior of individual chloroplasts and it has been found that these organelles can move in any direction without turning. This implies that any part of the chloroplast periphery can function as the leading or trailing edge during movement. This ability is mediated by a special structure, which consists of short actin filaments that are polymerized at the leading edge of moving chloroplasts and are specifically localized in the space between the chloroplast and the plasma membrane, and is called chloroplast-actin. In addition, several of the genes that encode proteins that are involved in chloroplast-actin polymerization or maintenance have been identified. In this Review, we discuss the mechanisms that regulate chloroplast movements through polymerization of the chloroplast-actin and propose a model for actin-driven chloroplast photorelocation movement.</P>

Multivalent mannose-decorated NIR nanoprobes for targeting pan lymph nodes

Wada, Hideyuki,Hyun, Hoon,Bao, Kai,Lee, Jeong Heon,El Fakhri, Georges,Choi, Yongdoo,Choi, Hak Soo Elsevier 2018 CHEMICAL ENGINEERING JOURNAL -LAUSANNE- Vol.340 No.-

<P><B>Abstract</B></P> <P>Lymphadenectomy is a prerequisite for most malignancies to define the precise staging of cancer, as well as resect the possible metastases completely. While it improves prognosis, lymphadenectomy often causes postoperative edema or bleeding because of unclear surgical margins. In this study, we synthesized near-infrared (NIR) fluorescent nanoprobes with conjugating various mannose moieties on the surface to target macrophages in the lymph node. Armed with these NIR nanoprobes, we demonstrated the feasibility of intraoperative pan lymph nodes (PLN) mapping and real-time optical imaging under the NIR fluorescence imaging system. We found that even single mannose-conjugated ZW800-1 showed specific uptake in lymph nodes within 4 h, and multiple mannose-employed polyrotaxanes highlighted PLN efficiently with low background signals in major organs. This technology can help surgeons perform lymphadenectomy with ease and safety by identifying all regional lymph nodes proficiently after a single intravenous injection of NIR nanoprobes.</P> <P><B>Highlights</B></P> <P> <UL> <LI> NIR fluorescent nanoprobes to target macrophages in the lymph node. </LI> <LI> Renal clearable nanoprobes enhance the SBR of target tissue. </LI> <LI> Intraoperative pan lymph nodes mapping is demonstrated. </LI> <LI> Surgeons can perform lymphadenectomy with ease and safety using NIR. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Gas permeation properties of c-plane aligned hexagonal tungsten oxide membranes formed by multi-stage synthesis

Wada Shintaro,Hagio Takeshi,Kunishi Hiroto,Park Jae-Hyeok,Phouthavong Vanpaseuth,Yamada Yuta,Terao Toshihiro,Li Xinling,Nijpanich Supinya,Ichino Ryoichi 한국세라믹학회 2023 한국세라믹학회지 Vol.60 No.6

Crystalline microporous membranes are promising tools for gas separation because their molecular dimension pores allow us to accurately sieve molecules by size. Recently, microporous hexagonal WO3 was reported as a new potential membrane material and its membrane was found to enable separation of water from water/acetic acid mixtures. Pore size of hexagonal WO3 seemed to be also suitable for separation of small gasses; however, its gas permeation properties have not been reported. Additionally, densification of membranes by decreasing intercrystalline gaps are extremely important in gas separation. One effective method to densify polycrystalline membranes is to repeat membrane synthesis, namely multi-stage synthesis. Here, we attempt to prepare dense hexagonal WO3 membranes on porous tubular supports by multi-stage synthesis and examined their densification and gas permeation properties. Densification was confirmed by permeation of SF6 (gas molecule larger than pores of hexagonal WO3), and its potential for separating small gasses was considered from single gas permeation of He and He/SF6 permselectivity. The results indicated that the multi-stage synthesis is effective to densify the membranes and He/SF6 permselectivity reached 42.8 for three-stage synthesis under modified conditions, implying hexagonal WO3 is a potential membrane material for small gas separation.

Correlation between Fat Desposit in Eye Muscle and Concentration of Plasma Lipoprotein in Fattened Steer(日文)

Wada.H. 江原大學校 農科大學 農業科學硏究所 1984 강원 농업생명환경연구 Vol.1 No.-

ROLE OF SINGLE PHOTON EMISSION COMPUTED TOMOGRAPHY FOR DETECTING LIVER TUMORS

Wada, Makoto,Ichiya, Yuichi,Kuwabara, Yasuo,Ayabe, Zenji,Katsuragi, Makoto,Matsuura, Keiichi 대한핵의학회 1984 핵의학 분자영상 Vol.18 No.1

외국문학으로서의 이태준 문학 - 일본문학과의 차이화(差異化)

WADA Tomomi 상허학회 1999 상허학보 Vol.5 No.-

The purpose of this paper is to analyze the characteristic features of the past studies in Japan on Lee Tae-jun and to investigate, based on the results of the analysis, what types of studies on Lee Tae-jun would be possible particularly in Japan. It was in 1941 that the Japanese first showed an interest in Lee Tae-jun’s literary works, when the Japanese translation of the collection of his novels was published. There was no Korean novelists but two, Lee Kwang-Su and Lee Tae-jun, whose literary works were translated into Japanese in the colonial days. The most definite reason why the Japanese became interestes in Lee Tae-jun was that his short stories showed a high level of completion. Therefore, the postwar study in Japan on Lee Tae-jun was also started by clarifying the structure of the mental world of the novelist, which lead his literary works to a high level of completion. Shokichi Cho paid attention to the fact that though Lee Tae-jun had aimed at becoming an activist in a racial movement in his youth, his failure in attaining his wish caused him to be a novelist. According to Cho, the motive for Lee Tae-jun’s aiming at becoming an activist in a racial movement was his wish to assimilate himself to his dead father. If such assimilation had been attained. Lee Tae-jun should have been liberated from his sense of being an orphan. However, his wish could not be fulfilled under the colonial circumstances at that time, and he was resigned to his fate as an orphan, and at the same time, to the fate as a novelist, a special profession allowed to the special fate as an orphan. It can be considered that Shokichi Cho’s understanding of Lee Tae-jun’s literary works has greatly been affected by his experience in Japanese literature. In the Japanese literary world, there was a novelist whose mental structure was poles apart from Lee Tae-jun’s. It was Naoya Shiga. His father was a living existence that oppressed him. Therefore, Shiga’s literary works were always created through his sense of conflict with his father. In Lee Tae-jun’s Iiterary works, we often find the situations in which the hero/heroine is unable to act in accordance with the dictates of his/her own sense. The reason for it can be clarified by making a comparison between his literary works and Shiga’s. In Shiga’s literary works, the hero/heroine acts in accordance with the dictates of his/her own sense. Hero’s/heroine’s decision is always made with confidence, as Shiga’s reaction to his living father. On the other hand, Lee Tae-jun created a virtual image of his dead father and tried to assimilate himself to it. In the background of hero's/heroine’s decision lies Lee Tae-jun’S vurtual image of his dead father. Therefore, such a decision cannot be made with confidence. The next step of the study in Japan on Lee Tae-jun was to clarify the philosophy that threaded through his short stories and long novels. Lee Tae-jun’s short stories have been highly estimated, while his long novels have always been estimated low. Toshikatsu Saegusa analyzed the personalities of the characters in Lee Tae-jun's long novels and classified them into several types. Through this work, it was cIarified that the most important motif for Lee Tae-jun’s long novels was the betrayal of love. This betrayal is characterized by the failure of the hero/heroine not only in love but also in his/her economic and social life. The hero/heroine, who failed in his/her life, is left behind the society and falls into a state just like an orphan. At this point of time, the characters in short stories and those in long novels are in the same situation. However, the characters in long novels do not disappear from the society without doing anything as in the case of those in short stories. They change their passion for love into the passion for trying to reform the society. Based on his analysis, Saegusa suggested that the reason for such an action is that they substituted love a...

A CHARACTERIZATION OF THE VANISHING OF THE SECOND PLURIGENUS FOR NORMAL SURFACE SINGULARITIES

Wada, Koukichi Korean Mathematical Society 2008 대한수학회보 Vol.45 No.2

In the study of normal (complex analytic) surface singularities, it is interesting to investigate the invariants. The purpose of this paper is to give a characterization of the vanishing of ${\delta}_2$. In [11], we gave characterizations of minimally elliptic singularities and rational triple points in terms of th.. second plurigenera ${\delta}_2$ and ${\gamma}_2$. In this paper, we also give a characterization of rational triple points in terms of a certain computation sequence. To prove our main theorems, we give two formulae for ${\delta}_2$ and ${\gamma}_2$ of rational surface singularities.

도일 후 손창섭의 법적 지위—사망 전후 발행 공적 서류를 근거로

WADA Tomomi 한국현대문학회 2023 한국현대문학연구 Vol.- No.70

This article attempts to verify the legal status of Son Chang-seop at Japan. It is commonly believed that he was naturalized in Japan, but no legal documents have been found to prove his naturalization. The only evidence is the testimony of his wife. The purpose of this article was to reveal his nationality based on several official documents issued in Japan before and after his death in 2010. In addition to the official documents, with reviewing his serialized novel “Yumaeng”, the aspects of the life for him and his family at Japan are clarified. Their life is known to have been very frugal. This is because he and his wife lived in a small public housing. However, the Son’s family lived in the apartment in Tokyo 23 wards at first, and then moved to a public housing in their 50s. Considering the special significance of living in a public complex in the 1980s Japan, this cannot be attributed to economic difficulties. At that time, residents of public housing tended to live temporarily to save money on living expenses while aiming for desirable housing. Similarly, for Son’s family, living in a public housing was a passage to a desirable living environment in later life. However, Son himself did not accept his family’s suggestion and died in a public housing. As for the nationality issue, there is no legal evidence that the family’s wishes were fulfilled. In public documents issued before and after his death in 2010, Son was listed only as a foreign spouse of a Japanese. Japanese are removed from family register upon death. However, there is no record that Son was removed from family register. This indicates that he was never a Japanese citizen. Only the person himself can change his nationality, and it is not possible to change it after death. This brings us to the conclusion of our discussion of Son’s nationality.

CLINICAL EVALUATION OF POSITRON EMISSION TOMOGRAPHY USING O-15C02 , O-1502 AND C-11CO

Wada, Makoto,Ichiya, Yuichi,Kuwabara, Yasuo,Ayabe, Zenji,Katsuragi, Makoto,Matsuura, Keiichi 대한핵의학회 1984 핵의학 분자영상 Vol.18 No.1

The effect of daily calf stroking frequency during the postnatal period on the establishment of the human-calf relationship

Wada, Satoko,Fukasawa, Michiru,Chiba, Takashi,Shishido, Tetsuro,Tozawa, Akitsu,Ogura, Shin-ichiro Asian Australasian Association of Animal Productio 2021 Animal Bioscience Vol.34 No.10

Objective: Stroking calves during the postnatal period could effectively improve human-calf relationships. The objective of this study is to examine how daily calf stroking frequency during the postnatal period affects the establishment of human-calf relationships. Methods: Six calves were stroked by a trainer for 6 minutes once daily for 5 days after birth (D1). Six calves were stroked by a trainer for 3 minutes twice daily for 5 days after birth (D2). A further four calves were stared at but not stroked as the control group. The overall stroking or staring duration was the same for all groups, at 6 min/d and 30 min over 5 days. The tests for reactions to the stationary trainer in an unfamiliar environment and avoidance distance measurements for an approaching trainer were conducted at 1 month and 3 months after the treatment. Results: Calves in both stroking groups approached significantly closer to the stationary trainer, vocalized less, and looked at the trainer shorter than the control group at 1 month. However, at 3 months, there was no significant difference between the D1 and the control group, whereas the D2 approached significantly closer to the trainer and vocalized less, and looked at the trainer for a shorter time than the control group. For the avoidance distance, the trainer could approach closer to both stroking groups than the control at 1 month, however, there was no difference among groups at 3 months. Conclusion: Our results suggested that the difference in the calf stroking procedure affected the established human-calf relationships, even though the total stroking duration was the same for all stroked calves. It is likely to be more effective to stroke more frequently than intensively when the aim is to establish better human-calf relationships within limited labor time.