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Quyen Van Nguyen,Hang Thi Khuat,Yen‑Ngoc Thi Nguyen,Dung Thi Vu,Thu‑Ha Bui,부경환 한국식물생명공학회 2020 Plant biotechnology reports Vol.14 No.5
Drynaria bonii H. Christ is a medicinal plant that has traditionally been used for the treatment of chronic diseases and tuberculosis. However, D. bonii has not yet been cultivated or propagated. In this study, we optimized the conditions for the spore culture of D. bonii to produce sporophytes. First, we evaluated the efects of various factors, such as temperature, light intensity, potting material, and pH, on spore germination and early gametophyte development. Under optimal culture conditions, 95–99% of the spores germinated within 2 weeks of culture. Next, based on these results, we established a culture system for gametophyte development and sporophyte production. Mature gametophytes frst appeared 5 months post-germination (mpg), and at 12 mpg, the rate of production of heart-shaped gametophytes reached 56.6%. Sporophytes at the early frst-leaf stage frst appeared at 6 mpg, and at 12 mpg, the rate of formation of sporophytes reached 15.4%. Interestingly, 6.3% of all gametophytes produced multiple sporophytes. Additionally, 42–62% of the gametophytes also produced multiple secondary gametophytes, indicating a high potential of D. bonii gametophytes to generate new gametophytes and, subsequently, sporophytes. When transferred to new trays or pots, sporophytes grew well and showed 100% survival. Overall, we conclude that this spore culture system can be successfully used for the propagation of D. bonii sporophytes.
( Vu Thi Thu Hang ),( Dinh Thi Quyen ),( Thi Tuyet Dao ),( Sy Le Thanh Nguyen ) 한국미생물 · 생명공학회 2012 Journal of microbiology and biotechnology Vol.22 No.3
A novel gene coding for an endo-β-1,4-mannanase (manA) from Bacillus subtilis strain G1 was cloned and overexpressed in P. pastoris GS115, and the enzyme was purified and characterized. The manA gene consisted of an open reading frame of 1,092 nucleotides, encoding a 364-aa protein, with a predicted molecular mass of 41 kDa. The β-mannanase showed an identity of 90.2-92.9% (≤95%) with the corresponding amino acid sequences from B. subtilis strains deposited in GenBank. The purified β- mannanase was a monomeric protein on SDS-PAGE with a specific activity of 2,718 U/mg and identified by MALDITOF mass spectrometry. The recombinant β-mannanase had an optimum temperature of 45oC and optimum pH of 6.5. The enzyme was stable at temperatures up to 50oC (for 8 h) and in the pH range of 5-9. EDTA and most tested metal ions showed a slightly to an obviously inhibitory effect on enzyme activity, whereas metal ions (Hg2+, Pb2+, and Co2+) substantially inhibited the recombinant β-mannanase. The chemical additives including detergents (Triton X- 100, Tween 20, and SDS) and organic solvents (methanol, ethanol, n-butanol, and acetone) decreased the enzyme activity, and especially no enzyme activity was observed by addition of SDS at the concentrations of 0.25-1.0% (w/v) or n-butanol at the concentrations of 20-30% (v/v). These results suggested that the β-mannanase expressed in P. pastoris could potentially be used as an additive in the feed for monogastric animals.
Dinh, Thi Hinh,Tran, Vu Diem Ngoc,Nguyen, Thi Thao,Hoang, Quyen Thi Ngoc,Han, Hyoung–,Su,Lee, Jae–,Shin Elsevier 2017 CERAMICS INTERNATIONAL Vol.43 No.18
<P><B>Abstract</B></P> <P>This study investigated the microstructure, dielectric, ferroelectric, and strain properties of lead‒free 0.995Bi<SUB>1/2</SUB>(Na<SUB>0.82</SUB>K<SUB>0.18</SUB>)<SUB>1/2</SUB>TiO<SUB>3</SUB>–0.005BaZrO<SUB>3</SUB>/0.98Bi<SUB>1/2</SUB>(Na<SUB>0.78</SUB>K<SUB>0.22</SUB>)<SUB>1/2</SUB>TiO<SUB>3</SUB>–0.02LaFeO<SUB>3</SUB> (BNKT18BZ/BNKT22LF) ceramic composites. We found that the required electric field for trigging the phase transition from ergodic relaxor (ER) to ferroelectric of BNKT18BZ/BNKT22LF ceramic composites can be effectively decreased by adding BNKT18BZ as nonergodic relaxor (NER) with changes of three different stabilized regions and the highest <I>S</I> <SUB>usable</SUB> peak points as a function of the applied electric field. The highest <I>d</I> <SUB>33</SUB> <SUP>*</SUP> value of 690p.m./V was obtained at 4kV/mm for 0.3wt BNKT18BZ fraction ceramic composites. This value was higher than 575 p.m./V of BNKT22LF ceramics under an applied electric field of 5kV/mm.</P>