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- 저자 : Vladan Ondřej (검색결과 2 건)
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Chromatin Structural Rearrangement during Dedifferentiation of Protoplasts of Cucumis sativus L.
Vladan Ondřej,Miloslav Kitner,Ivana Doležalová,Petr Nádvorník,Božena Navrátilová,Aleš Lebeda 한국분자세포생물학회 2009 Molecules and cells Vol.27 No.4
This paper reports on the structural rearrangement of satellite DNA type I repeats and heterochromatin during the dedifferentiation and cell cycling of mesophyll protoplasts of cucumber (Cucumis sativus). These repeats were localized in the telomeric heterochromatin of cucumber chromosomes and in the chromocenters of interphase nuclei. The dramatic reduction of heterochromatin involves decondensation of subtelomeric repeats in freshly isolated protoplasts; however, there are not a great many remarkable changes in the expression profile. In spite of that, reformation of the chromocenters, occurring 48 h after protoplast isolation, is accompanied by recondensation of satellite DNA type I; however, only partial reassembly of these repeats was revealed. In this study, FISH and a flow cytometry assay show a correlation between the partial chromocenter and the repeats reassembly, and with the re-entry of cultivated protoplasts into the cell cycle and first cell division. After that, divided cells displayed a higher variability in the expression profile than did leaves’ mesophyll cells and protoplasts.
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Lamin A/C and Polymeric Actin in Genome Organization
Vladan Ondřej,Emilie Lukášová,Jana Krejčí,Pavel Matula,Stanislav Kozubek 한국분자세포생물학회 2008 Molecules and cells Vol.26 No.4
In this work, we have studied the structural and functional linkage between lamin A/C, nuclear actin, and organization of chromosome territories (CTs) in mammary carcinoma MCF-7 cells. Selective down-regulation of lamin A/C expression led to disruption of the lamin A/C perinuclear layer and disorganization of lamin-bound emerin complexes at the inner nuclear membrane. The silencing of lamin A/C expression resulted in a decrease in the volume and surface area of chromosome territories, especially in chromosomes with high heterochromatin content. Inhibition of actin polymerization led to relaxation of the structure of chromosome territories, and an increase in the volumes and surface areas of the chromosome territories of human chromosomes 1, 2 and 13. The results show an important role of polymeric actin in the organization of the nuclei and the chromosome territories.
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