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      • KCI등재

        Primo Vascular Node in the Bone Marrow and Longevity

        Vitaly Vodyanoy,Oleg Pustovyy,Ludmila Globa 사단법인약침학회 2022 Journal of Acupuncture & Meridian Studies Vol.15 No.1

        Background: Intra-organic bone marrow node is predicted to be a part of the primo vascular system that plays a critical role in hematopoiesis and generation and regeneration of other cells. Two models of cell regeneration were suggested, one involving DNA synthesis and the other pertaining to DNA recycling. Objectives: The aim of this work is to extract a primo node from bone marrow, characterize its structure, understand its biochemistry and cell composition, and suggest a cell regeneration mechanism. Methods: Primo nodes were sampled from segmented halves of the rat femur. We used immunohistochemistry and high-resolution fluorescent microscopy to analyze 1200 samples obtained from 42 rats and 190 primo nodes. Results: Primo nodes in the bone marrow have an oval or round structure of about one millimeter in diameter, which is encompassed by a fine capsule, having incoming and outgoing vessels filled with the extracellular matrix and hematopoietic, mesenchymal, endothelial stem cells, as well as cells of the megakaryocyte family found in other primo nodes. Conclusion: Our findings imply that bone marrow nodes are intra-organic primo vascular nodes, and they provide ways and approaches for further investigation. Bone marrow nodes are simple to examine ex vivo in a variety of environments to assess cell regeneration mechanisms, wound healing, and organism rejuvenation and lifespan. Further research into these and other intra-organic nodes in animals and humans could lead to new regenerative medicine and longevity strategies that have yet to be discovered.

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        Lytic Phage-Based Magnetoelastic Biosensors for On-site Detection of Methicillin-Resistant <i>Staphylococcus aureus</i> on Spinach Leaves

        Byeon, Haeng Mi,Vodyanoy, Vitaly J.,Oh, Jun-Hyun,Kwon, Joong-Ho,Park, Mi-Kyung The Electrochemical Society 2015 Journal of the Electrochemical Society Vol.162 No.8

        <P>A lytic phage-based magnetoelastic (ME) biosensor method was firstly employed for on-site detection of methicillin-resistant <I>Staphylococcus aureus</I> (MRSA) on spinach leaves. Due to the virulent activity of this lytic phage, the effect of time and temperature on the extent of MRSA lysis was examined by incubating a mixture of the MRSA and lytic phage at various times (0, 15, 30, 45, 60, 75, and 90 min) and temperatures (0, 4, 15, 22, 37, and 45°C). After optimization of incubation time and temperature, spinach leaves were spiked with serial concentration of MRSA and the attachment of MRSA was confirmed using SEM. The phage-immobilized sensor and control (devoid of the lytic phage) sensor were placed on the surface of the leaves and the resonant frequency shifts of both sensors were compared. The optimal incubation time and temperature for contact of the phage-immobilized ME biosensor with the MRSA were determined to be ≤ 30 min and ≤ 22°C. The attachment of the lytic phage on the sensor was observed and the density of lytic phage on the sensor was determined to be 26 ± 3 particles/μm<SUP>2</SUP>. The resonant frequency shifts of the phage-immobilized sensor linearly increased with the increase in the MRSA concentration and had a correlation coefficient and slope of 0.819 and 840.9 Hz/log CFU, respectively. Detection limit was determined to be 1.76 log CFU/25 mm<SUP>2</SUP> surface of spinach.</P>

      • KCI등재

        Technical Challenges in Current Primo Vascular System Research and Potential Solutions

        Kyung Aih Kang,Claudio Maldonado,Vitaly Vodyanoy 사단법인약침학회 2016 Journal of Acupuncture & Meridian Studies Vol.9 No.6

        Since Bonghan Kim's discovery of the Bonghan system (BHS) in the 1960s, numerous reports have suggested that the system is fundamental for maintaining mammalian life. The BHS is a circulatory system independent of the blood or the lymphatic system, forms an extensive network throughout the entire mammalian body, has been reported to be the acupuncture meridian, stores distinct types of stem cells, and appears to have some roles in cancer metastasis. Despite Kim's first report having been published as early as 1962, research progress has been rather slow mainly because the system is very small and translucent, making it optically difficult to distinguish it from the hemoglobin-rich surrounding tissues. Unfortunately, Kim did not describe in detail the methods that he used for identifying and harvesting the system and the components of the system. In 2000, Kwang-Sup Soh reopened the BHS research, and since then, new and important scientific findings on the system have been reported, and many of Kim's results have been verified. In 2010, the BHS was renamed the primo vascular system. Nevertheless, good tools to properly deal with this system are still lacking. In this article, we address some of the technical difficulties involved in studying the primo vascular system and attempt to discuss potential ways to overcome those difficulties.

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