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        The Effect of BTX Compounds on the Biodegradation of ETBE by an ETBE Degrading Bacterial Consortium

        Vijayalakshmi Gunasekaran,Luke Stam,Magda Constantí 한국생물공학회 2013 Biotechnology and Bioprocess Engineering Vol.18 No.6

        Ethyl tert-butyl ether (ETBE) is a fuel oxygenatethat is commonly used in Europe to achieve completecombustion of automobile fuels and to control air pollution. It is potentially toxic and can enter the human system viacontaminated water bodies. In the present study, we haveidentified an enriched bacterial consortium from a gasolinecontaminatedsite that can degrade ETBE. Bacterialconsortium A was able to degrade 47% of the added ETBEin 4 days and it continued to degrade up to 51% in 9 days. Consortium A consisted of Xanthomonas sp., Methylibiumsp., Methylobacillus sp., and Methylovorus sp. which wereidentified as the participating bacteria during ETBEdegradation by DGGE - 16S rDNA analysis. In addition toETBE, this consortium degraded benzene, toluene andxylene isomers (BTX) when they were present as the solecarbon source. The degradation efficiency increasedpredominantly when ETBE was included as an additionalcarbon source. Interestingly, the degradation of ETBEdecreased to 14% in 9 days when present with BTXcompounds. We report that ETBE degradation is sloweddown or inhibited when BTX compounds are present. Thisis a crucial observation for ETBE degradation in the naturalenvironment.

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