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RISS 인기검색어
- 검색키워드
- 저자 : Venket (검색결과 3 건)
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Effect of Lycopene on Prostate LNCaP Cancer Cells in Culture
A. Venket Rao,Linda Kim,Leticia G. Rao 한국식품영양과학회 2002 Journal of medicinal food Vol.5 No.4
Epidemiological studies have shown an inverse relationship betwen serum lycopene levelsand the risk of prostate cancer. The objective of this study was to measure the effect of ly-copene on the proliferation of LNCaP human prostate cancer cells in culture. A new, water-dispersible lycopene in an apropriate vehicle was used. The stock solution was diluted inthe medium to obtain lycopene concentrations of 102 6, 102 5, and 102 4 M; their correspond-ing vehicles were similarly diluted to be used as controls. Cells were grown for 48 hours inRPMI-1640 medium suplemented with 10% fetal bovine serum and antibiotics. Lycopenewas then added at different concentrations, and the cells were allowed to grow for 24, 48, 72,and 96 hours. Lycopene at concentrations of 102 6 and 102 5 M significantly reduced the growthof LNCaP cells after 48, 72, and 96 hours of incubation, by 24.4% to 42.8% (P , .05). The in-hibitory effect of lycopene was significantly higher than that of the corresponding vehiclecontrols. In a follow-up experiment, a lower range of lycopene concentrations (102 9 to 102 7M) was used to determine whether there was a dose-response effect. Lycopene significantlydecreased the growth of cells in a dose-dependen t maner when cells were incubated for 24,48, 72, or 96 hours (F 5 3.150, 11.27, 54.51, and 297.5, respectively; P , .05). The growth in-hibitory effect of lycopene on human prostate cancer cells observed in this study suggests apossibly important role for lycopene as an antioxidant in human prostate cancer; however,investigations of other mechanisms are warranted.181
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Leticia G. Rao,Linda Kim,A. Venket Rao 한국식품영양과학회 2003 Journal of medicinal food Vol.6 No.2
We explored the possibility that lycopene, a carotenoid that is abundant in tomatoes, has ef-fects on proliferation and differentiation of osteoblasts, the cells responsible for bone for-mation. Human osteoblast-like osteosarcoma SaOS-2 cells were cultured for 24 hours, afterwhich varying doses of a water-dispersible microemulsion preparation of lycopene or vehi-cle of the same dilution were aded. The cells were further cultured for 24 to 14 hours, andthen the cell numbers were counted. Lycopene at 102 6 and 102 5 M had significant stimula-tory effects on cell numbers, compared with the corresponding vehicle treatment, at all timepoints from 24 to 14 hours. The effects of lycopene on activity of the differentiation markeralkaline phosphatase activity in the absence or presence of dexamethasone were shown to bedependent on the stage of cell differentiation. This is the first report on the effects of lycopeneon osteoblasts of human origin; the results may have important aplications in the preven-tion of osteoporosis.79
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Lycopene I―Effect on Osteoclasts:
Leticia G. Rao,Nupura Krishnadev,Katharine Banasikowska,A. Venket Rao 한국식품영양과학회 2003 Journal of medicinal food Vol.6 No.2
Osteoclasts have been shown to produce reactive oxygen species (ROS) that can stimulatebone resorption. We explored the hypothesis that lycopene, the antioxidant carotenoid fromtomatoes, can inhibit mineral resorption by inhibiting osteoclast formation and the produc-tion of ROS. Cells from bone marrow prepared from rat femur were plated into 16-well cal-cium phosphate coated Osteologic Multi-test Slides and cultured in a-minimal essentialmedium suplemented with dexamethasone, b-glycerophosphate, and ascorbic acid. The cellswere treated with varying doses of lycopene in the absence or presence of parathyroid hor-mone (PTH) at the start of culture and at each medium change (i.e., every 48 hours). On day8, mineral resorption pits were quantitated. Similar, parallel experiments were carried out in12 well plastic dishes to assess tartrate-resistant acid phosphatase (TRAP) activity. Resultsshowed that lycopene inhibited TRAP 1 formation of multinucleated cells in both vehicle-and PTH-treated cultures. Osteoclasts reduced nitroblue tetrazolium (NBT) to purple-coloredformazan, indicating the presence of ROS in these cells. The formazan-staing cells were de-creased by treatment with 102 5 M lycopene, indicating that lycopene inhibited the formationof ROS-secreting osteoclasts. In conclusion, we have shown that lycopene inhibits basal andPTH-stimulated osteoclastic mineral resorption and formation of TRAP 1 multinucleated os-teoclasts, as well as the ROS produced by osteoclasts. These findings are novel and may beimportant in the pathogenesis, treatment, and prevention of osteoporosis.69
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